Experiment: L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_670 and Pf6N2E2_671 are separated by 214 nucleotides Pf6N2E2_671 and Pf6N2E2_672 are separated by 51 nucleotides Pf6N2E2_672 and Pf6N2E2_673 overlap by 8 nucleotides
Pf6N2E2_670 - 1-pyrroline-4-hydroxy-2-carboxylate deaminase (EC 3.5.4.22), at 775,083 to 776,000
_670
Pf6N2E2_671 - 2-methylaconitate racemase, at 776,215 to 777,243
_671
Pf6N2E2_672 - ABC-type polar amino acid transport system, ATPase component, at 777,295 to 778,017
_672
Pf6N2E2_673 - Probable permease of ABC transporter, at 778,010 to 778,660
_673
Position (kb) 776
777
778 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 775.288 kb on + strand, within Pf6N2E2_670 at 775.288 kb on + strand at 775.289 kb on - strand, within Pf6N2E2_670 at 775.289 kb on - strand, within Pf6N2E2_670 at 775.289 kb on - strand, within Pf6N2E2_670 at 775.416 kb on + strand, within Pf6N2E2_670 at 775.416 kb on + strand, within Pf6N2E2_670 at 775.416 kb on + strand, within Pf6N2E2_670 at 775.417 kb on - strand, within Pf6N2E2_670 at 775.570 kb on + strand, within Pf6N2E2_670 at 775.570 kb on + strand, within Pf6N2E2_670 at 775.571 kb on - strand, within Pf6N2E2_670 at 775.571 kb on - strand, within Pf6N2E2_670 at 775.588 kb on + strand, within Pf6N2E2_670 at 775.589 kb on - strand, within Pf6N2E2_670 at 775.589 kb on - strand, within Pf6N2E2_670 at 775.589 kb on - strand, within Pf6N2E2_670 at 775.663 kb on + strand, within Pf6N2E2_670 at 775.663 kb on + strand, within Pf6N2E2_670 at 776.032 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.034 kb on + strand at 776.035 kb on - strand at 776.035 kb on - strand at 776.035 kb on - strand at 776.035 kb on - strand at 776.042 kb on + strand at 776.043 kb on - strand at 776.057 kb on + strand at 776.057 kb on + strand at 776.057 kb on + strand at 776.057 kb on + strand at 776.057 kb on + strand at 776.058 kb on - strand at 776.058 kb on - strand at 776.061 kb on + strand at 776.062 kb on - strand at 776.062 kb on - strand at 776.062 kb on - strand at 776.117 kb on + strand at 776.117 kb on + strand at 776.141 kb on + strand at 776.328 kb on - strand, within Pf6N2E2_671 at 776.406 kb on + strand, within Pf6N2E2_671 at 776.406 kb on + strand, within Pf6N2E2_671 at 776.454 kb on + strand, within Pf6N2E2_671 at 776.454 kb on + strand, within Pf6N2E2_671 at 776.739 kb on - strand, within Pf6N2E2_671 at 777.217 kb on + strand at 777.217 kb on + strand at 777.218 kb on - strand at 777.218 kb on - strand at 777.494 kb on - strand, within Pf6N2E2_672 at 777.548 kb on + strand, within Pf6N2E2_672 at 777.548 kb on + strand, within Pf6N2E2_672 at 777.549 kb on - strand, within Pf6N2E2_672 at 778.078 kb on - strand, within Pf6N2E2_673 at 778.078 kb on - strand, within Pf6N2E2_673 at 778.081 kb on - strand, within Pf6N2E2_673
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine (N) remove 775,288 + Pf6N2E2_670 0.22 -1.1 775,288 + +0.3 775,289 - Pf6N2E2_670 0.22 -0.7 775,289 - Pf6N2E2_670 0.22 -1.3 775,289 - Pf6N2E2_670 0.22 -0.5 775,416 + Pf6N2E2_670 0.36 -0.2 775,416 + Pf6N2E2_670 0.36 -3.4 775,416 + Pf6N2E2_670 0.36 -0.9 775,417 - Pf6N2E2_670 0.36 -1.1 775,570 + Pf6N2E2_670 0.53 -1.0 775,570 + Pf6N2E2_670 0.53 -0.1 775,571 - Pf6N2E2_670 0.53 +0.5 775,571 - Pf6N2E2_670 0.53 +0.6 775,588 + Pf6N2E2_670 0.55 -0.5 775,589 - Pf6N2E2_670 0.55 -1.7 775,589 - Pf6N2E2_670 0.55 +2.1 775,589 - Pf6N2E2_670 0.55 +0.6 775,663 + Pf6N2E2_670 0.63 -1.3 775,663 + Pf6N2E2_670 0.63 +0.1 776,032 + -0.7 776,034 + -0.9 776,034 + -0.2 776,034 + -0.4 776,034 + -0.4 776,034 + -0.4 776,034 + -0.7 776,034 + -0.3 776,035 - +0.4 776,035 - -0.5 776,035 - -0.7 776,035 - -0.6 776,042 + -0.3 776,043 - +0.5 776,057 + -0.7 776,057 + -0.3 776,057 + +1.4 776,057 + -0.1 776,057 + -0.4 776,058 - +1.0 776,058 - -2.5 776,061 + +0.1 776,062 - -0.2 776,062 - +0.5 776,062 - -2.1 776,117 + -0.3 776,117 + +0.2 776,141 + +0.5 776,328 - Pf6N2E2_671 0.11 -0.2 776,406 + Pf6N2E2_671 0.19 -0.1 776,406 + Pf6N2E2_671 0.19 -1.8 776,454 + Pf6N2E2_671 0.23 -1.4 776,454 + Pf6N2E2_671 0.23 -0.4 776,739 - Pf6N2E2_671 0.51 +1.3 777,217 + +0.1 777,217 + -0.3 777,218 - -2.3 777,218 - -1.4 777,494 - Pf6N2E2_672 0.28 -0.7 777,548 + Pf6N2E2_672 0.35 +0.7 777,548 + Pf6N2E2_672 0.35 +0.2 777,549 - Pf6N2E2_672 0.35 +0.5 778,078 - Pf6N2E2_673 0.10 -1.4 778,078 - Pf6N2E2_673 0.10 -0.4 778,081 - Pf6N2E2_673 0.11 -1.0
Or see this region's nucleotide sequence