Experiment: L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5 and Pf6N2E2_6 overlap by 4 nucleotides Pf6N2E2_6 and Pf6N2E2_7 are separated by 240 nucleotides Pf6N2E2_7 and Pf6N2E2_8 are separated by 265 nucleotides
Pf6N2E2_5 - Non-ribosomal peptide synthetase modules, pyoverdine @ Siderophore biosynthesis non-ribosomal peptide synthetase modules, at 20,966 to 27,997
_5
Pf6N2E2_6 - Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39), at 27,994 to 31,221
_6
Pf6N2E2_7 - hypothetical protein, at 31,462 to 31,596
_7
Pf6N2E2_8 - TonB-dependent siderophore receptor, at 31,862 to 34,336
_8
Position (kb) 27
28
29
30
31
32 Strain fitness (log2 ratio) -2
-1
0
1 at 27.233 kb on + strand, within Pf6N2E2_5 at 27.234 kb on - strand, within Pf6N2E2_5 at 27.323 kb on + strand at 27.323 kb on + strand at 27.324 kb on - strand at 27.324 kb on - strand at 27.383 kb on + strand at 27.656 kb on + strand at 27.675 kb on + strand at 27.676 kb on - strand at 27.728 kb on + strand at 27.728 kb on + strand at 27.729 kb on - strand at 27.729 kb on - strand at 27.729 kb on - strand at 27.729 kb on - strand at 27.729 kb on - strand at 27.731 kb on + strand at 27.732 kb on - strand at 27.785 kb on + strand at 27.785 kb on + strand at 27.785 kb on + strand at 27.786 kb on - strand at 27.910 kb on + strand at 27.910 kb on + strand at 27.910 kb on + strand at 27.911 kb on - strand at 27.911 kb on - strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.914 kb on + strand at 27.915 kb on - strand at 28.170 kb on - strand at 28.373 kb on + strand, within Pf6N2E2_6 at 28.373 kb on + strand, within Pf6N2E2_6 at 28.373 kb on + strand, within Pf6N2E2_6 at 28.376 kb on - strand, within Pf6N2E2_6 at 28.466 kb on + strand, within Pf6N2E2_6 at 28.466 kb on + strand, within Pf6N2E2_6 at 28.466 kb on + strand, within Pf6N2E2_6 at 28.467 kb on - strand, within Pf6N2E2_6 at 28.477 kb on + strand, within Pf6N2E2_6 at 28.477 kb on + strand, within Pf6N2E2_6 at 28.478 kb on - strand, within Pf6N2E2_6 at 28.478 kb on - strand, within Pf6N2E2_6 at 28.529 kb on - strand, within Pf6N2E2_6 at 28.586 kb on - strand, within Pf6N2E2_6 at 28.858 kb on + strand, within Pf6N2E2_6 at 28.858 kb on + strand, within Pf6N2E2_6 at 28.859 kb on - strand, within Pf6N2E2_6 at 29.182 kb on + strand, within Pf6N2E2_6 at 29.183 kb on - strand, within Pf6N2E2_6 at 29.183 kb on - strand, within Pf6N2E2_6 at 29.183 kb on - strand, within Pf6N2E2_6 at 29.200 kb on + strand, within Pf6N2E2_6 at 29.201 kb on - strand, within Pf6N2E2_6 at 29.236 kb on + strand, within Pf6N2E2_6 at 29.246 kb on + strand, within Pf6N2E2_6 at 29.247 kb on - strand, within Pf6N2E2_6 at 29.247 kb on - strand, within Pf6N2E2_6 at 29.369 kb on - strand, within Pf6N2E2_6 at 29.369 kb on - strand, within Pf6N2E2_6 at 29.403 kb on + strand, within Pf6N2E2_6 at 29.449 kb on + strand, within Pf6N2E2_6 at 29.450 kb on - strand, within Pf6N2E2_6 at 29.597 kb on - strand, within Pf6N2E2_6 at 29.794 kb on + strand, within Pf6N2E2_6 at 29.795 kb on - strand, within Pf6N2E2_6 at 29.805 kb on + strand, within Pf6N2E2_6 at 29.805 kb on + strand, within Pf6N2E2_6 at 29.805 kb on + strand, within Pf6N2E2_6 at 29.806 kb on - strand, within Pf6N2E2_6 at 29.806 kb on - strand, within Pf6N2E2_6 at 29.866 kb on + strand, within Pf6N2E2_6 at 30.325 kb on + strand, within Pf6N2E2_6 at 30.380 kb on - strand, within Pf6N2E2_6 at 30.475 kb on + strand, within Pf6N2E2_6 at 30.475 kb on + strand, within Pf6N2E2_6 at 30.476 kb on - strand, within Pf6N2E2_6 at 31.190 kb on + strand at 31.190 kb on + strand at 31.190 kb on + strand at 31.190 kb on + strand at 31.191 kb on - strand at 31.277 kb on + strand at 31.277 kb on + strand at 31.312 kb on + strand at 31.312 kb on + strand at 31.312 kb on + strand at 31.312 kb on + strand at 31.312 kb on + strand at 31.312 kb on + strand at 31.313 kb on - strand at 31.313 kb on - strand at 31.313 kb on - strand at 31.363 kb on + strand at 31.364 kb on - strand at 31.409 kb on - strand at 31.636 kb on + strand at 31.640 kb on + strand at 31.640 kb on + strand at 31.641 kb on - strand at 31.641 kb on - strand at 31.641 kb on - strand at 31.644 kb on + strand at 31.644 kb on + strand at 31.644 kb on + strand at 31.645 kb on - strand at 31.645 kb on - strand at 31.645 kb on - strand at 31.645 kb on - strand at 31.645 kb on - strand at 31.645 kb on - strand at 31.842 kb on + strand at 31.843 kb on - strand at 31.843 kb on - strand at 31.848 kb on + strand at 31.849 kb on - strand at 31.851 kb on + strand at 31.852 kb on - strand at 31.852 kb on - strand at 32.075 kb on + strand at 32.075 kb on + strand at 32.075 kb on + strand at 32.076 kb on - strand at 32.076 kb on - strand at 32.194 kb on + strand, within Pf6N2E2_8
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine (N) remove 27,233 + Pf6N2E2_5 0.89 -0.9 27,234 - Pf6N2E2_5 0.89 -0.6 27,323 + +0.1 27,323 + +0.0 27,324 - -0.8 27,324 - +0.5 27,383 + +0.3 27,656 + -1.4 27,675 + -1.1 27,676 - -0.4 27,728 + -0.3 27,728 + -0.5 27,729 - +1.0 27,729 - +0.1 27,729 - +1.2 27,729 - +0.1 27,729 - +0.1 27,731 + +0.3 27,732 - +0.4 27,785 + -0.1 27,785 + +0.9 27,785 + -0.1 27,786 - +0.3 27,910 + +1.3 27,910 + -0.0 27,910 + +0.2 27,911 - +0.4 27,911 - -2.0 27,914 + -0.4 27,914 + -0.7 27,914 + -0.2 27,914 + -0.2 27,914 + -0.9 27,914 + +0.4 27,914 + +1.5 27,914 + +0.4 27,915 - +0.6 28,170 - +0.4 28,373 + Pf6N2E2_6 0.12 +0.2 28,373 + Pf6N2E2_6 0.12 +1.3 28,373 + Pf6N2E2_6 0.12 -0.8 28,376 - Pf6N2E2_6 0.12 +0.0 28,466 + Pf6N2E2_6 0.15 +0.1 28,466 + Pf6N2E2_6 0.15 -0.2 28,466 + Pf6N2E2_6 0.15 -0.2 28,467 - Pf6N2E2_6 0.15 -0.1 28,477 + Pf6N2E2_6 0.15 -0.1 28,477 + Pf6N2E2_6 0.15 -1.2 28,478 - Pf6N2E2_6 0.15 +0.5 28,478 - Pf6N2E2_6 0.15 +0.4 28,529 - Pf6N2E2_6 0.17 -1.3 28,586 - Pf6N2E2_6 0.18 +0.3 28,858 + Pf6N2E2_6 0.27 -0.2 28,858 + Pf6N2E2_6 0.27 +0.3 28,859 - Pf6N2E2_6 0.27 -0.5 29,182 + Pf6N2E2_6 0.37 +0.4 29,183 - Pf6N2E2_6 0.37 +0.0 29,183 - Pf6N2E2_6 0.37 -0.2 29,183 - Pf6N2E2_6 0.37 -0.5 29,200 + Pf6N2E2_6 0.37 -0.2 29,201 - Pf6N2E2_6 0.37 -1.2 29,236 + Pf6N2E2_6 0.38 -0.3 29,246 + Pf6N2E2_6 0.39 +1.0 29,247 - Pf6N2E2_6 0.39 -0.0 29,247 - Pf6N2E2_6 0.39 -0.6 29,369 - Pf6N2E2_6 0.43 +0.9 29,369 - Pf6N2E2_6 0.43 -0.2 29,403 + Pf6N2E2_6 0.44 -0.4 29,449 + Pf6N2E2_6 0.45 +0.2 29,450 - Pf6N2E2_6 0.45 -0.7 29,597 - Pf6N2E2_6 0.50 -0.2 29,794 + Pf6N2E2_6 0.56 -1.8 29,795 - Pf6N2E2_6 0.56 -0.4 29,805 + Pf6N2E2_6 0.56 -0.1 29,805 + Pf6N2E2_6 0.56 -0.3 29,805 + Pf6N2E2_6 0.56 -0.6 29,806 - Pf6N2E2_6 0.56 +0.3 29,806 - Pf6N2E2_6 0.56 +1.0 29,866 + Pf6N2E2_6 0.58 +0.8 30,325 + Pf6N2E2_6 0.72 -1.0 30,380 - Pf6N2E2_6 0.74 -0.8 30,475 + Pf6N2E2_6 0.77 -1.2 30,475 + Pf6N2E2_6 0.77 -0.1 30,476 - Pf6N2E2_6 0.77 -1.4 31,190 + -0.7 31,190 + +0.3 31,190 + +0.1 31,190 + +0.4 31,191 - -0.0 31,277 + -0.2 31,277 + +0.6 31,312 + -0.1 31,312 + -0.7 31,312 + +0.3 31,312 + -1.0 31,312 + +0.2 31,312 + +1.2 31,313 - -0.6 31,313 - +0.3 31,313 - -0.5 31,363 + +1.2 31,364 - +0.4 31,409 - -0.9 31,636 + -2.6 31,640 + -0.3 31,640 + -1.5 31,641 - +1.1 31,641 - +0.3 31,641 - +1.1 31,644 + -0.5 31,644 + -1.8 31,644 + +0.3 31,645 - -1.0 31,645 - +0.0 31,645 - +0.4 31,645 - -1.2 31,645 - -0.8 31,645 - +0.5 31,842 + +0.1 31,843 - +0.4 31,843 - +0.1 31,848 + -0.2 31,849 - -0.1 31,851 + +0.1 31,852 - +1.1 31,852 - +0.4 32,075 + +1.1 32,075 + +0.2 32,075 + +0.3 32,076 - -0.8 32,076 - +0.5 32,194 + Pf6N2E2_8 0.13 -0.3
Or see this region's nucleotide sequence