Experiment: L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5441 and Pf6N2E2_5442 are separated by 495 nucleotides Pf6N2E2_5442 and Pf6N2E2_5443 overlap by 4 nucleotides Pf6N2E2_5443 and Pf6N2E2_5444 are separated by 88 nucleotides
Pf6N2E2_5441 - FIG00955104: hypothetical protein, at 6,248,059 to 6,248,289
_5441
Pf6N2E2_5442 - Glucose dehydrogenase, PQQ-dependent (EC 1.1.5.2), at 6,248,785 to 6,251,199
_5442
Pf6N2E2_5443 - hypothetical protein, at 6,251,196 to 6,251,357
_5443
Pf6N2E2_5444 - Phosphoribosylglycinamide formyltransferase 2 (EC 2.1.2.-), at 6,251,446 to 6,252,627
_5444
Position (kb)
6248
6249
6250
6251
6252 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6247.962 kb on - strand at 6247.962 kb on - strand at 6247.962 kb on - strand at 6247.962 kb on - strand at 6247.962 kb on - strand at 6248.093 kb on + strand, within Pf6N2E2_5441 at 6248.093 kb on + strand, within Pf6N2E2_5441 at 6248.094 kb on - strand, within Pf6N2E2_5441 at 6248.216 kb on + strand, within Pf6N2E2_5441 at 6248.216 kb on + strand, within Pf6N2E2_5441 at 6248.216 kb on + strand, within Pf6N2E2_5441 at 6248.216 kb on + strand, within Pf6N2E2_5441 at 6248.216 kb on + strand, within Pf6N2E2_5441 at 6248.216 kb on + strand, within Pf6N2E2_5441 at 6248.217 kb on - strand, within Pf6N2E2_5441 at 6248.217 kb on - strand, within Pf6N2E2_5441 at 6248.217 kb on - strand, within Pf6N2E2_5441 at 6248.217 kb on - strand, within Pf6N2E2_5441 at 6248.313 kb on + strand at 6248.356 kb on + strand at 6248.356 kb on + strand at 6248.356 kb on + strand at 6248.357 kb on - strand at 6248.357 kb on - strand at 6248.357 kb on - strand at 6248.436 kb on - strand at 6248.436 kb on - strand at 6248.492 kb on + strand at 6248.734 kb on + strand at 6248.734 kb on + strand at 6248.734 kb on + strand at 6248.734 kb on + strand at 6248.735 kb on - strand at 6248.735 kb on - strand at 6248.735 kb on - strand at 6248.743 kb on + strand at 6248.743 kb on + strand at 6248.743 kb on + strand at 6248.743 kb on + strand at 6248.743 kb on + strand at 6248.743 kb on + strand at 6248.744 kb on - strand at 6248.744 kb on - strand at 6248.744 kb on - strand at 6248.744 kb on - strand at 6248.762 kb on - strand at 6248.784 kb on + strand at 6248.785 kb on - strand at 6248.785 kb on - strand at 6248.785 kb on - strand at 6248.785 kb on - strand at 6248.785 kb on - strand at 6248.849 kb on + strand at 6248.849 kb on + strand at 6248.849 kb on + strand at 6248.849 kb on + strand at 6248.849 kb on + strand at 6248.850 kb on - strand at 6248.850 kb on - strand at 6248.850 kb on - strand at 6248.850 kb on - strand at 6248.850 kb on - strand at 6248.850 kb on - strand at 6248.914 kb on + strand at 6248.914 kb on + strand at 6248.914 kb on + strand at 6248.914 kb on + strand at 6248.915 kb on - strand at 6248.915 kb on - strand at 6249.397 kb on + strand, within Pf6N2E2_5442 at 6249.397 kb on + strand, within Pf6N2E2_5442 at 6249.398 kb on - strand, within Pf6N2E2_5442 at 6249.398 kb on - strand, within Pf6N2E2_5442 at 6249.481 kb on + strand, within Pf6N2E2_5442 at 6249.481 kb on + strand, within Pf6N2E2_5442 at 6249.481 kb on + strand, within Pf6N2E2_5442 at 6249.481 kb on + strand, within Pf6N2E2_5442 at 6249.481 kb on + strand, within Pf6N2E2_5442 at 6249.482 kb on - strand, within Pf6N2E2_5442 at 6249.482 kb on - strand, within Pf6N2E2_5442 at 6249.482 kb on - strand, within Pf6N2E2_5442 at 6249.482 kb on - strand, within Pf6N2E2_5442 at 6249.482 kb on - strand, within Pf6N2E2_5442 at 6249.482 kb on - strand, within Pf6N2E2_5442 at 6249.482 kb on - strand, within Pf6N2E2_5442 at 6249.622 kb on + strand, within Pf6N2E2_5442 at 6249.623 kb on - strand, within Pf6N2E2_5442 at 6249.623 kb on - strand, within Pf6N2E2_5442 at 6249.641 kb on - strand, within Pf6N2E2_5442 at 6249.641 kb on - strand, within Pf6N2E2_5442 at 6249.844 kb on + strand, within Pf6N2E2_5442 at 6249.844 kb on + strand, within Pf6N2E2_5442 at 6249.845 kb on - strand, within Pf6N2E2_5442 at 6249.845 kb on - strand, within Pf6N2E2_5442 at 6250.030 kb on + strand, within Pf6N2E2_5442 at 6250.030 kb on + strand, within Pf6N2E2_5442 at 6250.030 kb on + strand, within Pf6N2E2_5442 at 6250.030 kb on + strand, within Pf6N2E2_5442 at 6250.031 kb on - strand, within Pf6N2E2_5442 at 6250.031 kb on - strand, within Pf6N2E2_5442 at 6250.031 kb on - strand, within Pf6N2E2_5442 at 6250.031 kb on - strand, within Pf6N2E2_5442 at 6250.031 kb on - strand, within Pf6N2E2_5442 at 6250.094 kb on - strand, within Pf6N2E2_5442 at 6250.159 kb on + strand, within Pf6N2E2_5442 at 6250.307 kb on + strand, within Pf6N2E2_5442 at 6250.308 kb on - strand, within Pf6N2E2_5442 at 6250.337 kb on - strand, within Pf6N2E2_5442 at 6250.337 kb on - strand, within Pf6N2E2_5442 at 6250.543 kb on + strand, within Pf6N2E2_5442 at 6250.544 kb on - strand, within Pf6N2E2_5442 at 6250.544 kb on - strand, within Pf6N2E2_5442 at 6250.669 kb on + strand, within Pf6N2E2_5442 at 6250.669 kb on + strand, within Pf6N2E2_5442 at 6250.669 kb on + strand, within Pf6N2E2_5442 at 6250.670 kb on - strand, within Pf6N2E2_5442 at 6250.670 kb on - strand, within Pf6N2E2_5442 at 6250.670 kb on - strand, within Pf6N2E2_5442 at 6250.670 kb on - strand, within Pf6N2E2_5442 at 6250.670 kb on - strand, within Pf6N2E2_5442 at 6250.749 kb on - strand, within Pf6N2E2_5442 at 6250.749 kb on - strand, within Pf6N2E2_5442 at 6250.749 kb on - strand, within Pf6N2E2_5442 at 6250.749 kb on - strand, within Pf6N2E2_5442 at 6250.749 kb on - strand, within Pf6N2E2_5442 at 6250.837 kb on + strand, within Pf6N2E2_5442 at 6251.009 kb on - strand at 6251.020 kb on + strand at 6251.020 kb on + strand at 6251.020 kb on + strand at 6251.021 kb on - strand at 6251.021 kb on - strand at 6251.021 kb on - strand at 6251.116 kb on + strand at 6251.116 kb on + strand at 6251.116 kb on + strand at 6251.116 kb on + strand at 6251.116 kb on + strand at 6251.116 kb on + strand at 6251.116 kb on + strand at 6251.116 kb on + strand at 6251.116 kb on + strand at 6251.117 kb on - strand at 6251.117 kb on - strand at 6251.117 kb on - strand at 6251.117 kb on - strand at 6251.117 kb on - strand at 6251.170 kb on + strand at 6251.170 kb on + strand at 6251.170 kb on + strand at 6251.170 kb on + strand at 6251.171 kb on - strand at 6251.171 kb on - strand at 6251.171 kb on - strand at 6251.171 kb on - strand at 6251.197 kb on + strand at 6251.447 kb on + strand at 6251.447 kb on + strand at 6251.447 kb on + strand at 6251.448 kb on - strand at 6251.448 kb on - strand at 6252.089 kb on + strand, within Pf6N2E2_5444 at 6252.090 kb on - strand, within Pf6N2E2_5444 at 6252.090 kb on - strand, within Pf6N2E2_5444
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine (N) remove 6,247,962 - -0.3 6,247,962 - -0.3 6,247,962 - +0.8 6,247,962 - -0.7 6,247,962 - -2.1 6,248,093 + Pf6N2E2_5441 0.15 +1.5 6,248,093 + Pf6N2E2_5441 0.15 -0.4 6,248,094 - Pf6N2E2_5441 0.15 -0.3 6,248,216 + Pf6N2E2_5441 0.68 +1.6 6,248,216 + Pf6N2E2_5441 0.68 -0.3 6,248,216 + Pf6N2E2_5441 0.68 +0.8 6,248,216 + Pf6N2E2_5441 0.68 -0.4 6,248,216 + Pf6N2E2_5441 0.68 +0.4 6,248,216 + Pf6N2E2_5441 0.68 -0.1 6,248,217 - Pf6N2E2_5441 0.68 -0.7 6,248,217 - Pf6N2E2_5441 0.68 -0.5 6,248,217 - Pf6N2E2_5441 0.68 -0.1 6,248,217 - Pf6N2E2_5441 0.68 +1.3 6,248,313 + +0.9 6,248,356 + -0.2 6,248,356 + +1.7 6,248,356 + -0.5 6,248,357 - -1.6 6,248,357 - +1.7 6,248,357 - +2.3 6,248,436 - -0.2 6,248,436 - -0.3 6,248,492 + -0.1 6,248,734 + +0.7 6,248,734 + +1.0 6,248,734 + +0.1 6,248,734 + -0.1 6,248,735 - -1.8 6,248,735 - -0.8 6,248,735 - +0.2 6,248,743 + +0.2 6,248,743 + -0.3 6,248,743 + +0.3 6,248,743 + +0.7 6,248,743 + -0.2 6,248,743 + +1.1 6,248,744 - -0.2 6,248,744 - +0.2 6,248,744 - -1.3 6,248,744 - -1.4 6,248,762 - +0.3 6,248,784 + +0.1 6,248,785 - -0.1 6,248,785 - -1.6 6,248,785 - -1.9 6,248,785 - -0.6 6,248,785 - -1.0 6,248,849 + -0.8 6,248,849 + +0.2 6,248,849 + -0.4 6,248,849 + +1.7 6,248,849 + +0.1 6,248,850 - +0.3 6,248,850 - +0.7 6,248,850 - -0.0 6,248,850 - -0.1 6,248,850 - +0.7 6,248,850 - +0.2 6,248,914 + -1.2 6,248,914 + -0.8 6,248,914 + -1.1 6,248,914 + +1.4 6,248,915 - -1.1 6,248,915 - -0.1 6,249,397 + Pf6N2E2_5442 0.25 -0.8 6,249,397 + Pf6N2E2_5442 0.25 +1.1 6,249,398 - Pf6N2E2_5442 0.25 -0.9 6,249,398 - Pf6N2E2_5442 0.25 -3.3 6,249,481 + Pf6N2E2_5442 0.29 -1.3 6,249,481 + Pf6N2E2_5442 0.29 -0.8 6,249,481 + Pf6N2E2_5442 0.29 -0.2 6,249,481 + Pf6N2E2_5442 0.29 -1.1 6,249,481 + Pf6N2E2_5442 0.29 -0.9 6,249,482 - Pf6N2E2_5442 0.29 -0.6 6,249,482 - Pf6N2E2_5442 0.29 -0.5 6,249,482 - Pf6N2E2_5442 0.29 -1.8 6,249,482 - Pf6N2E2_5442 0.29 -1.4 6,249,482 - Pf6N2E2_5442 0.29 -1.3 6,249,482 - Pf6N2E2_5442 0.29 -0.9 6,249,482 - Pf6N2E2_5442 0.29 -1.1 6,249,622 + Pf6N2E2_5442 0.35 -2.5 6,249,623 - Pf6N2E2_5442 0.35 -2.0 6,249,623 - Pf6N2E2_5442 0.35 +0.3 6,249,641 - Pf6N2E2_5442 0.35 -0.3 6,249,641 - Pf6N2E2_5442 0.35 -0.6 6,249,844 + Pf6N2E2_5442 0.44 +0.5 6,249,844 + Pf6N2E2_5442 0.44 -0.1 6,249,845 - Pf6N2E2_5442 0.44 -2.1 6,249,845 - Pf6N2E2_5442 0.44 -0.6 6,250,030 + Pf6N2E2_5442 0.52 +0.1 6,250,030 + Pf6N2E2_5442 0.52 -1.1 6,250,030 + Pf6N2E2_5442 0.52 -0.9 6,250,030 + Pf6N2E2_5442 0.52 -1.2 6,250,031 - Pf6N2E2_5442 0.52 -1.0 6,250,031 - Pf6N2E2_5442 0.52 -0.3 6,250,031 - Pf6N2E2_5442 0.52 -1.2 6,250,031 - Pf6N2E2_5442 0.52 -0.4 6,250,031 - Pf6N2E2_5442 0.52 -1.0 6,250,094 - Pf6N2E2_5442 0.54 -1.5 6,250,159 + Pf6N2E2_5442 0.57 -0.8 6,250,307 + Pf6N2E2_5442 0.63 -1.8 6,250,308 - Pf6N2E2_5442 0.63 +0.1 6,250,337 - Pf6N2E2_5442 0.64 -1.5 6,250,337 - Pf6N2E2_5442 0.64 -1.5 6,250,543 + Pf6N2E2_5442 0.73 -1.8 6,250,544 - Pf6N2E2_5442 0.73 -0.6 6,250,544 - Pf6N2E2_5442 0.73 +0.0 6,250,669 + Pf6N2E2_5442 0.78 -0.4 6,250,669 + Pf6N2E2_5442 0.78 -0.1 6,250,669 + Pf6N2E2_5442 0.78 -1.6 6,250,670 - Pf6N2E2_5442 0.78 -1.8 6,250,670 - Pf6N2E2_5442 0.78 +0.0 6,250,670 - Pf6N2E2_5442 0.78 -1.4 6,250,670 - Pf6N2E2_5442 0.78 -0.9 6,250,670 - Pf6N2E2_5442 0.78 -2.2 6,250,749 - Pf6N2E2_5442 0.81 -1.0 6,250,749 - Pf6N2E2_5442 0.81 -2.5 6,250,749 - Pf6N2E2_5442 0.81 -0.5 6,250,749 - Pf6N2E2_5442 0.81 -1.2 6,250,749 - Pf6N2E2_5442 0.81 -0.0 6,250,837 + Pf6N2E2_5442 0.85 -0.6 6,251,009 - -2.2 6,251,020 + -0.8 6,251,020 + -0.3 6,251,020 + -0.3 6,251,021 - +0.1 6,251,021 - -2.4 6,251,021 - -0.6 6,251,116 + -0.1 6,251,116 + -1.6 6,251,116 + -0.6 6,251,116 + -0.3 6,251,116 + -1.0 6,251,116 + -2.7 6,251,116 + -2.2 6,251,116 + -0.1 6,251,116 + -0.8 6,251,117 - -1.4 6,251,117 - -0.6 6,251,117 - -1.1 6,251,117 - -1.8 6,251,117 - -1.3 6,251,170 + -0.7 6,251,170 + -0.9 6,251,170 + -1.5 6,251,170 + -0.3 6,251,171 - -1.3 6,251,171 - +0.1 6,251,171 - -1.3 6,251,171 - -0.7 6,251,197 + -0.6 6,251,447 + -0.7 6,251,447 + +0.0 6,251,447 + -1.1 6,251,448 - +0.6 6,251,448 - +0.2 6,252,089 + Pf6N2E2_5444 0.54 -0.0 6,252,090 - Pf6N2E2_5444 0.54 +0.3 6,252,090 - Pf6N2E2_5444 0.54 -0.5
Or see this region's nucleotide sequence