Experiment: L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2040 and Pf6N2E2_2041 are separated by 7 nucleotides Pf6N2E2_2041 and Pf6N2E2_2042 are separated by 190 nucleotides Pf6N2E2_2042 and Pf6N2E2_2043 are separated by 43 nucleotides
Pf6N2E2_2040 - D-beta-hydroxybutyrate permease, at 2,429,187 to 2,430,578
_2040
Pf6N2E2_2041 - D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30), at 2,430,586 to 2,431,362
_2041
Pf6N2E2_2042 - Acetoacetyl-CoA synthetase (EC 6.2.1.16), at 2,431,553 to 2,433,508
_2042
Pf6N2E2_2043 - Twitching motility protein PilT, at 2,433,552 to 2,434,739
_2043
Position (kb)
2431
2432
2433
2434 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2430.559 kb on - strand at 2431.045 kb on + strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.046 kb on - strand, within Pf6N2E2_2041 at 2431.292 kb on + strand at 2431.293 kb on - strand at 2431.293 kb on - strand at 2431.293 kb on - strand at 2431.335 kb on + strand at 2431.336 kb on - strand at 2431.361 kb on - strand at 2431.498 kb on + strand at 2431.499 kb on - strand at 2431.502 kb on + strand at 2431.503 kb on - strand at 2431.503 kb on - strand at 2431.503 kb on - strand at 2431.503 kb on - strand at 2431.524 kb on + strand at 2431.524 kb on + strand at 2431.524 kb on + strand at 2431.525 kb on - strand at 2431.525 kb on - strand at 2431.525 kb on - strand at 2432.231 kb on + strand, within Pf6N2E2_2042 at 2432.232 kb on - strand, within Pf6N2E2_2042 at 2432.354 kb on + strand, within Pf6N2E2_2042 at 2432.354 kb on + strand, within Pf6N2E2_2042 at 2432.354 kb on + strand, within Pf6N2E2_2042 at 2432.354 kb on + strand, within Pf6N2E2_2042 at 2432.355 kb on - strand, within Pf6N2E2_2042 at 2432.355 kb on - strand, within Pf6N2E2_2042 at 2432.355 kb on - strand, within Pf6N2E2_2042 at 2432.364 kb on - strand, within Pf6N2E2_2042 at 2432.483 kb on + strand, within Pf6N2E2_2042 at 2432.484 kb on - strand, within Pf6N2E2_2042 at 2432.484 kb on - strand, within Pf6N2E2_2042 at 2432.484 kb on - strand, within Pf6N2E2_2042 at 2432.484 kb on - strand, within Pf6N2E2_2042 at 2432.486 kb on + strand, within Pf6N2E2_2042 at 2432.486 kb on + strand, within Pf6N2E2_2042 at 2432.487 kb on - strand, within Pf6N2E2_2042 at 2432.487 kb on - strand, within Pf6N2E2_2042 at 2432.559 kb on - strand, within Pf6N2E2_2042 at 2432.559 kb on - strand, within Pf6N2E2_2042 at 2432.749 kb on + strand, within Pf6N2E2_2042 at 2432.750 kb on - strand, within Pf6N2E2_2042 at 2432.750 kb on - strand, within Pf6N2E2_2042 at 2432.750 kb on - strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.753 kb on + strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.754 kb on - strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.759 kb on + strand, within Pf6N2E2_2042 at 2432.760 kb on - strand, within Pf6N2E2_2042 at 2432.760 kb on - strand, within Pf6N2E2_2042 at 2432.760 kb on - strand, within Pf6N2E2_2042 at 2432.760 kb on - strand, within Pf6N2E2_2042 at 2432.760 kb on - strand, within Pf6N2E2_2042 at 2432.766 kb on - strand, within Pf6N2E2_2042 at 2433.029 kb on + strand, within Pf6N2E2_2042 at 2433.030 kb on - strand, within Pf6N2E2_2042 at 2433.030 kb on - strand, within Pf6N2E2_2042 at 2433.069 kb on - strand, within Pf6N2E2_2042 at 2433.367 kb on + strand at 2433.367 kb on + strand at 2433.368 kb on - strand at 2433.560 kb on + strand at 2433.614 kb on + strand at 2434.176 kb on - strand, within Pf6N2E2_2043 at 2434.177 kb on + strand, within Pf6N2E2_2043 at 2434.177 kb on + strand, within Pf6N2E2_2043 at 2434.177 kb on + strand, within Pf6N2E2_2043 at 2434.177 kb on + strand, within Pf6N2E2_2043 at 2434.178 kb on - strand, within Pf6N2E2_2043 at 2434.178 kb on - strand, within Pf6N2E2_2043 at 2434.178 kb on - strand, within Pf6N2E2_2043 at 2434.320 kb on + strand, within Pf6N2E2_2043
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine (N) remove 2,430,559 - +0.6 2,431,045 + Pf6N2E2_2041 0.59 -0.4 2,431,046 - Pf6N2E2_2041 0.59 +0.2 2,431,046 - Pf6N2E2_2041 0.59 -0.7 2,431,046 - Pf6N2E2_2041 0.59 -0.9 2,431,046 - Pf6N2E2_2041 0.59 -0.1 2,431,046 - Pf6N2E2_2041 0.59 +0.0 2,431,292 + -2.0 2,431,293 - -1.7 2,431,293 - +0.4 2,431,293 - +2.8 2,431,335 + -0.4 2,431,336 - -1.1 2,431,361 - +1.0 2,431,498 + +0.2 2,431,499 - -1.1 2,431,502 + +0.2 2,431,503 - -0.1 2,431,503 - -0.8 2,431,503 - -0.8 2,431,503 - -1.3 2,431,524 + +0.6 2,431,524 + +0.6 2,431,524 + -1.0 2,431,525 - +0.8 2,431,525 - +0.7 2,431,525 - -0.2 2,432,231 + Pf6N2E2_2042 0.35 -1.2 2,432,232 - Pf6N2E2_2042 0.35 -1.3 2,432,354 + Pf6N2E2_2042 0.41 -2.0 2,432,354 + Pf6N2E2_2042 0.41 +0.0 2,432,354 + Pf6N2E2_2042 0.41 -0.8 2,432,354 + Pf6N2E2_2042 0.41 -0.9 2,432,355 - Pf6N2E2_2042 0.41 -0.3 2,432,355 - Pf6N2E2_2042 0.41 +0.5 2,432,355 - Pf6N2E2_2042 0.41 +1.2 2,432,364 - Pf6N2E2_2042 0.41 +0.9 2,432,483 + Pf6N2E2_2042 0.48 -0.6 2,432,484 - Pf6N2E2_2042 0.48 -0.8 2,432,484 - Pf6N2E2_2042 0.48 -0.4 2,432,484 - Pf6N2E2_2042 0.48 +0.5 2,432,484 - Pf6N2E2_2042 0.48 -0.2 2,432,486 + Pf6N2E2_2042 0.48 +0.2 2,432,486 + Pf6N2E2_2042 0.48 -0.3 2,432,487 - Pf6N2E2_2042 0.48 -1.0 2,432,487 - Pf6N2E2_2042 0.48 -0.0 2,432,559 - Pf6N2E2_2042 0.51 -0.3 2,432,559 - Pf6N2E2_2042 0.51 +0.4 2,432,749 + Pf6N2E2_2042 0.61 +1.2 2,432,750 - Pf6N2E2_2042 0.61 -1.1 2,432,750 - Pf6N2E2_2042 0.61 -0.5 2,432,750 - Pf6N2E2_2042 0.61 +0.3 2,432,753 + Pf6N2E2_2042 0.61 -1.1 2,432,753 + Pf6N2E2_2042 0.61 -1.1 2,432,753 + Pf6N2E2_2042 0.61 +0.3 2,432,753 + Pf6N2E2_2042 0.61 -2.1 2,432,753 + Pf6N2E2_2042 0.61 +2.5 2,432,753 + Pf6N2E2_2042 0.61 -0.3 2,432,754 - Pf6N2E2_2042 0.61 +0.3 2,432,754 - Pf6N2E2_2042 0.61 +0.2 2,432,754 - Pf6N2E2_2042 0.61 -0.9 2,432,754 - Pf6N2E2_2042 0.61 -0.2 2,432,754 - Pf6N2E2_2042 0.61 -0.1 2,432,754 - Pf6N2E2_2042 0.61 +1.2 2,432,759 + Pf6N2E2_2042 0.62 -0.2 2,432,759 + Pf6N2E2_2042 0.62 -0.2 2,432,759 + Pf6N2E2_2042 0.62 -1.2 2,432,759 + Pf6N2E2_2042 0.62 -0.2 2,432,759 + Pf6N2E2_2042 0.62 +0.9 2,432,759 + Pf6N2E2_2042 0.62 +0.6 2,432,760 - Pf6N2E2_2042 0.62 -0.2 2,432,760 - Pf6N2E2_2042 0.62 +0.3 2,432,760 - Pf6N2E2_2042 0.62 -1.2 2,432,760 - Pf6N2E2_2042 0.62 -0.6 2,432,760 - Pf6N2E2_2042 0.62 +0.0 2,432,766 - Pf6N2E2_2042 0.62 +0.8 2,433,029 + Pf6N2E2_2042 0.75 -0.2 2,433,030 - Pf6N2E2_2042 0.76 -1.6 2,433,030 - Pf6N2E2_2042 0.76 -0.8 2,433,069 - Pf6N2E2_2042 0.78 +0.1 2,433,367 + -0.2 2,433,367 + -0.4 2,433,368 - +0.2 2,433,560 + -0.3 2,433,614 + -0.5 2,434,176 - Pf6N2E2_2043 0.53 +0.0 2,434,177 + Pf6N2E2_2043 0.53 +0.3 2,434,177 + Pf6N2E2_2043 0.53 -0.6 2,434,177 + Pf6N2E2_2043 0.53 -0.6 2,434,177 + Pf6N2E2_2043 0.53 -1.2 2,434,178 - Pf6N2E2_2043 0.53 -0.8 2,434,178 - Pf6N2E2_2043 0.53 +1.0 2,434,178 - Pf6N2E2_2043 0.53 +0.1 2,434,320 + Pf6N2E2_2043 0.65 +0.3
Or see this region's nucleotide sequence