Experiment: L-Lysine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1221 and Pf6N2E2_1222 are separated by 148 nucleotides Pf6N2E2_1222 and Pf6N2E2_1223 are separated by 153 nucleotides Pf6N2E2_1223 and Pf6N2E2_1224 are separated by 114 nucleotides
Pf6N2E2_1221 - sodium/hydrogen exchanger family protein, at 1,427,660 to 1,428,997
_1221
Pf6N2E2_1222 - FIG00954310: hypothetical protein, at 1,429,146 to 1,429,682
_1222
Pf6N2E2_1223 - Amino acid transporters, at 1,429,836 to 1,430,066
_1223
Pf6N2E2_1224 - Two-component hybrid sensor and regulator, at 1,430,181 to 1,432,109
_1224
Position (kb)
1429
1430
1431 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1428.842 kb on + strand, within Pf6N2E2_1221 at 1428.842 kb on + strand, within Pf6N2E2_1221 at 1428.971 kb on + strand at 1428.971 kb on + strand at 1428.971 kb on + strand at 1428.972 kb on - strand at 1428.999 kb on + strand at 1429.173 kb on - strand at 1429.174 kb on + strand at 1429.175 kb on - strand at 1429.175 kb on - strand at 1429.175 kb on - strand at 1429.175 kb on - strand at 1429.183 kb on + strand at 1429.183 kb on + strand at 1429.226 kb on + strand, within Pf6N2E2_1222 at 1429.226 kb on + strand, within Pf6N2E2_1222 at 1429.226 kb on + strand, within Pf6N2E2_1222 at 1429.226 kb on + strand, within Pf6N2E2_1222 at 1429.226 kb on + strand, within Pf6N2E2_1222 at 1429.227 kb on - strand, within Pf6N2E2_1222 at 1429.227 kb on - strand, within Pf6N2E2_1222 at 1429.227 kb on - strand, within Pf6N2E2_1222 at 1429.304 kb on - strand, within Pf6N2E2_1222 at 1429.335 kb on + strand, within Pf6N2E2_1222 at 1429.534 kb on + strand, within Pf6N2E2_1222 at 1429.534 kb on + strand, within Pf6N2E2_1222 at 1429.534 kb on + strand, within Pf6N2E2_1222 at 1429.534 kb on + strand, within Pf6N2E2_1222 at 1429.534 kb on + strand, within Pf6N2E2_1222 at 1429.535 kb on - strand, within Pf6N2E2_1222 at 1429.535 kb on - strand, within Pf6N2E2_1222 at 1429.535 kb on - strand, within Pf6N2E2_1222 at 1429.672 kb on - strand at 1429.720 kb on - strand at 1429.809 kb on - strand at 1429.809 kb on - strand at 1429.809 kb on - strand at 1429.843 kb on - strand at 1429.946 kb on + strand, within Pf6N2E2_1223 at 1429.958 kb on + strand, within Pf6N2E2_1223 at 1429.958 kb on + strand, within Pf6N2E2_1223 at 1429.985 kb on + strand, within Pf6N2E2_1223 at 1430.183 kb on - strand at 1430.183 kb on - strand at 1430.282 kb on - strand at 1430.407 kb on + strand, within Pf6N2E2_1224 at 1430.613 kb on - strand, within Pf6N2E2_1224 at 1430.798 kb on - strand, within Pf6N2E2_1224 at 1430.989 kb on - strand, within Pf6N2E2_1224 at 1431.027 kb on - strand, within Pf6N2E2_1224 at 1431.061 kb on + strand, within Pf6N2E2_1224
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine (N) remove 1,428,842 + Pf6N2E2_1221 0.88 +0.2 1,428,842 + Pf6N2E2_1221 0.88 +1.5 1,428,971 + -0.0 1,428,971 + +0.5 1,428,971 + +0.2 1,428,972 - +2.9 1,428,999 + -1.1 1,429,173 - +1.1 1,429,174 + -0.2 1,429,175 - +1.0 1,429,175 - +2.9 1,429,175 - -0.6 1,429,175 - +1.3 1,429,183 + -0.6 1,429,183 + -0.3 1,429,226 + Pf6N2E2_1222 0.15 +0.1 1,429,226 + Pf6N2E2_1222 0.15 +1.4 1,429,226 + Pf6N2E2_1222 0.15 +0.6 1,429,226 + Pf6N2E2_1222 0.15 +0.0 1,429,226 + Pf6N2E2_1222 0.15 +0.3 1,429,227 - Pf6N2E2_1222 0.15 -1.0 1,429,227 - Pf6N2E2_1222 0.15 +0.4 1,429,227 - Pf6N2E2_1222 0.15 +0.4 1,429,304 - Pf6N2E2_1222 0.29 +0.3 1,429,335 + Pf6N2E2_1222 0.35 +1.6 1,429,534 + Pf6N2E2_1222 0.72 -0.5 1,429,534 + Pf6N2E2_1222 0.72 -0.1 1,429,534 + Pf6N2E2_1222 0.72 +0.5 1,429,534 + Pf6N2E2_1222 0.72 +0.4 1,429,534 + Pf6N2E2_1222 0.72 +0.3 1,429,535 - Pf6N2E2_1222 0.72 -0.3 1,429,535 - Pf6N2E2_1222 0.72 -1.4 1,429,535 - Pf6N2E2_1222 0.72 +0.6 1,429,672 - -0.3 1,429,720 - +1.6 1,429,809 - +0.6 1,429,809 - -1.1 1,429,809 - +0.4 1,429,843 - +0.0 1,429,946 + Pf6N2E2_1223 0.48 -1.9 1,429,958 + Pf6N2E2_1223 0.53 -0.3 1,429,958 + Pf6N2E2_1223 0.53 -0.4 1,429,985 + Pf6N2E2_1223 0.65 -0.7 1,430,183 - -0.3 1,430,183 - +1.2 1,430,282 - +0.1 1,430,407 + Pf6N2E2_1224 0.12 -0.9 1,430,613 - Pf6N2E2_1224 0.22 +0.2 1,430,798 - Pf6N2E2_1224 0.32 -0.5 1,430,989 - Pf6N2E2_1224 0.42 +1.2 1,431,027 - Pf6N2E2_1224 0.44 +1.1 1,431,061 + Pf6N2E2_1224 0.46 +0.6
Or see this region's nucleotide sequence