Experiment: Growth in M5G
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00718 and CCNA_03930 are separated by 6 nucleotides CCNA_03930 and CCNA_03931 are separated by 542 nucleotides CCNA_03931 and CCNA_03932 are separated by 2 nucleotides CCNA_03932 and CCNA_03933 are separated by 30 nucleotides CCNA_03933 and CCNA_00719 are separated by 115 nucleotides
CCNA_00718: CCNA_00718 - abortive infection protein, at 771,887 to 772,783
_00718
CCNA_03930: CCNA_03930 - hypothetical protein, at 772,790 to 772,894
_03930
CCNA_03931: CCNA_03931 - hypothetical protein, at 773,437 to 773,730
_03931
CCNA_03932: CCNA_03932 - hypothetical protein, at 773,733 to 773,987
_03932
CCNA_03933: CCNA_03933 - hypothetical protein, at 774,018 to 774,278
_03933
CCNA_00719: CCNA_00719 - type I secretion adaptor protein hlyD, at 774,394 to 775,635
_00719
Position (kb) 773
774 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 772.747 kb on + strand at 772.752 kb on + strand at 772.795 kb on - strand at 772.795 kb on - strand at 772.795 kb on - strand at 772.795 kb on - strand at 772.808 kb on - strand, within CCNA_03930 at 772.812 kb on + strand, within CCNA_03930 at 772.842 kb on + strand, within CCNA_03930 at 772.847 kb on + strand, within CCNA_03930 at 772.850 kb on - strand, within CCNA_03930 at 772.852 kb on + strand at 772.860 kb on - strand, within CCNA_03930 at 772.860 kb on - strand, within CCNA_03930 at 772.866 kb on + strand, within CCNA_03930 at 772.869 kb on - strand, within CCNA_03930 at 772.874 kb on - strand, within CCNA_03930 at 772.874 kb on - strand, within CCNA_03930 at 772.897 kb on - strand at 772.956 kb on + strand at 772.956 kb on + strand at 772.964 kb on - strand at 772.969 kb on + strand at 772.985 kb on + strand at 772.985 kb on + strand at 772.991 kb on + strand at 772.991 kb on + strand at 772.991 kb on + strand at 772.999 kb on - strand at 772.999 kb on - strand at 772.999 kb on - strand at 772.999 kb on - strand at 773.010 kb on - strand at 773.030 kb on - strand at 773.076 kb on - strand at 773.080 kb on + strand at 773.094 kb on + strand at 773.095 kb on - strand at 773.101 kb on - strand at 773.102 kb on - strand at 773.102 kb on - strand at 773.102 kb on - strand at 773.118 kb on - strand at 773.120 kb on + strand at 773.126 kb on - strand at 773.126 kb on - strand at 773.128 kb on - strand at 773.161 kb on + strand at 773.184 kb on - strand at 773.192 kb on + strand at 773.193 kb on + strand at 773.193 kb on + strand at 773.202 kb on - strand at 773.202 kb on - strand at 773.213 kb on + strand at 773.213 kb on + strand at 773.221 kb on - strand at 773.221 kb on - strand at 773.248 kb on + strand at 773.248 kb on + strand at 773.248 kb on + strand at 773.248 kb on + strand at 773.249 kb on + strand at 773.249 kb on + strand at 773.249 kb on + strand at 773.249 kb on + strand at 773.249 kb on + strand at 773.256 kb on - strand at 773.256 kb on - strand at 773.257 kb on - strand at 773.257 kb on - strand at 773.257 kb on - strand at 773.257 kb on - strand at 773.257 kb on - strand at 773.257 kb on - strand at 773.257 kb on - strand at 773.257 kb on - strand at 773.268 kb on - strand at 773.269 kb on - strand at 773.270 kb on + strand at 773.270 kb on + strand at 773.271 kb on + strand at 773.278 kb on - strand at 773.279 kb on - strand at 773.279 kb on - strand at 773.309 kb on - strand at 773.309 kb on - strand at 773.309 kb on - strand at 773.732 kb on + strand at 773.732 kb on + strand at 773.740 kb on - strand at 773.740 kb on - strand at 773.740 kb on - strand at 773.742 kb on + strand at 773.749 kb on + strand at 773.767 kb on - strand, within CCNA_03932 at 773.791 kb on - strand, within CCNA_03932 at 773.793 kb on + strand, within CCNA_03932 at 773.793 kb on + strand, within CCNA_03932 at 773.801 kb on - strand, within CCNA_03932 at 773.801 kb on - strand, within CCNA_03932 at 773.801 kb on - strand, within CCNA_03932 at 773.801 kb on - strand, within CCNA_03932 at 773.801 kb on - strand, within CCNA_03932 at 773.828 kb on + strand, within CCNA_03932 at 773.833 kb on + strand, within CCNA_03932 at 773.841 kb on - strand, within CCNA_03932 at 773.851 kb on - strand, within CCNA_03932 at 773.870 kb on - strand, within CCNA_03932 at 773.891 kb on + strand, within CCNA_03932 at 773.895 kb on - strand, within CCNA_03932 at 773.924 kb on + strand, within CCNA_03932 at 773.932 kb on - strand, within CCNA_03932 at 773.932 kb on - strand, within CCNA_03932 at 773.935 kb on + strand, within CCNA_03932 at 773.936 kb on + strand, within CCNA_03932 at 773.936 kb on + strand, within CCNA_03932 at 773.936 kb on + strand, within CCNA_03932 at 773.944 kb on - strand, within CCNA_03932 at 773.944 kb on - strand, within CCNA_03932 at 773.944 kb on - strand, within CCNA_03932 at 773.946 kb on + strand, within CCNA_03932 at 773.946 kb on + strand, within CCNA_03932 at 773.974 kb on - strand at 774.001 kb on + strand at 774.057 kb on + strand, within CCNA_03933 at 774.057 kb on + strand, within CCNA_03933 at 774.060 kb on + strand, within CCNA_03933 at 774.090 kb on - strand, within CCNA_03933 at 774.090 kb on - strand, within CCNA_03933 at 774.101 kb on + strand, within CCNA_03933 at 774.107 kb on + strand, within CCNA_03933 at 774.109 kb on - strand, within CCNA_03933 at 774.119 kb on + strand, within CCNA_03933 at 774.120 kb on - strand, within CCNA_03933 at 774.124 kb on + strand, within CCNA_03933 at 774.124 kb on + strand, within CCNA_03933 at 774.134 kb on + strand, within CCNA_03933 at 774.148 kb on + strand, within CCNA_03933 at 774.188 kb on + strand, within CCNA_03933 at 774.192 kb on + strand, within CCNA_03933 at 774.207 kb on + strand, within CCNA_03933 at 774.214 kb on + strand, within CCNA_03933 at 774.285 kb on + strand at 774.285 kb on + strand at 774.293 kb on + strand at 774.293 kb on - strand at 774.293 kb on - strand at 774.293 kb on - strand at 774.405 kb on + strand at 774.448 kb on + strand at 774.467 kb on + strand at 774.475 kb on - strand at 774.511 kb on + strand at 774.516 kb on + strand at 774.535 kb on - strand, within CCNA_00719 at 774.596 kb on - strand, within CCNA_00719 at 774.626 kb on - strand, within CCNA_00719 at 774.685 kb on + strand, within CCNA_00719 at 774.703 kb on + strand, within CCNA_00719 at 774.743 kb on + strand, within CCNA_00719 at 774.743 kb on + strand, within CCNA_00719 at 774.743 kb on + strand, within CCNA_00719 at 774.756 kb on + strand, within CCNA_00719 at 774.764 kb on - strand, within CCNA_00719 at 774.838 kb on + strand, within CCNA_00719 at 774.838 kb on + strand, within CCNA_00719 at 774.838 kb on + strand, within CCNA_00719 at 774.838 kb on + strand, within CCNA_00719 at 774.838 kb on + strand, within CCNA_00719 at 774.838 kb on + strand, within CCNA_00719 at 774.838 kb on + strand, within CCNA_00719 at 774.845 kb on + strand, within CCNA_00719 at 774.845 kb on + strand, within CCNA_00719 at 774.940 kb on + strand, within CCNA_00719 at 774.940 kb on + strand, within CCNA_00719 at 774.948 kb on - strand, within CCNA_00719 at 774.975 kb on - strand, within CCNA_00719
Per-strain Table
Position Strand Gene LocusTag Fraction Growth in M5G remove 772,747 + +0.3 772,752 + +0.0 772,795 - +0.2 772,795 - +0.4 772,795 - +0.6 772,795 - +0.4 772,808 - CCNA_03930 0.17 +1.5 772,812 + CCNA_03930 0.21 +0.2 772,842 + CCNA_03930 0.50 +0.1 772,847 + CCNA_03930 0.54 +0.2 772,850 - CCNA_03930 0.57 +0.5 772,852 + -0.8 772,860 - CCNA_03930 0.67 +0.2 772,860 - CCNA_03930 0.67 -0.0 772,866 + CCNA_03930 0.72 +0.3 772,869 - CCNA_03930 0.75 +0.4 772,874 - CCNA_03930 0.80 +0.5 772,874 - CCNA_03930 0.80 -0.1 772,897 - +0.1 772,956 + +0.8 772,956 + -0.7 772,964 - -0.7 772,969 + -0.7 772,985 + -0.2 772,985 + +0.1 772,991 + +0.7 772,991 + -0.7 772,991 + +0.6 772,999 - +0.7 772,999 - +0.2 772,999 - +0.4 772,999 - -0.0 773,010 - +0.1 773,030 - +0.2 773,076 - +0.3 773,080 + -1.0 773,094 + -0.1 773,095 - +0.8 773,101 - +0.4 773,102 - +0.5 773,102 - +0.2 773,102 - +0.1 773,118 - +0.6 773,120 + +0.1 773,126 - +0.8 773,126 - +0.0 773,128 - +0.4 773,161 + -0.1 773,184 - +0.4 773,192 + -0.4 773,193 + -1.1 773,193 + -0.4 773,202 - +0.2 773,202 - -0.2 773,213 + -1.5 773,213 + -0.7 773,221 - +0.5 773,221 - +0.7 773,248 + +0.0 773,248 + -0.3 773,248 + +2.3 773,248 + -0.5 773,249 + -0.2 773,249 + -0.1 773,249 + -0.0 773,249 + -2.2 773,249 + +0.9 773,256 - +0.7 773,256 - +0.1 773,257 - +0.3 773,257 - +0.3 773,257 - +0.5 773,257 - +0.5 773,257 - -0.1 773,257 - -0.5 773,257 - +0.4 773,257 - +0.5 773,268 - +0.5 773,269 - +0.6 773,270 + +0.7 773,270 + -1.4 773,271 + +0.1 773,278 - +0.7 773,279 - +0.5 773,279 - +0.6 773,309 - +0.1 773,309 - +0.8 773,309 - +0.5 773,732 + -0.6 773,732 + -0.5 773,740 - -0.5 773,740 - +0.7 773,740 - +0.5 773,742 + -1.6 773,749 + -2.1 773,767 - CCNA_03932 0.13 -3.0 773,791 - CCNA_03932 0.23 -0.3 773,793 + CCNA_03932 0.24 -0.9 773,793 + CCNA_03932 0.24 +0.5 773,801 - CCNA_03932 0.27 -0.8 773,801 - CCNA_03932 0.27 -3.1 773,801 - CCNA_03932 0.27 -2.8 773,801 - CCNA_03932 0.27 -1.4 773,801 - CCNA_03932 0.27 -2.4 773,828 + CCNA_03932 0.37 -1.9 773,833 + CCNA_03932 0.39 -1.2 773,841 - CCNA_03932 0.42 -1.5 773,851 - CCNA_03932 0.46 -2.2 773,870 - CCNA_03932 0.54 -2.1 773,891 + CCNA_03932 0.62 -2.5 773,895 - CCNA_03932 0.64 -0.3 773,924 + CCNA_03932 0.75 -0.6 773,932 - CCNA_03932 0.78 -2.9 773,932 - CCNA_03932 0.78 -3.4 773,935 + CCNA_03932 0.79 -2.0 773,936 + CCNA_03932 0.80 -1.4 773,936 + CCNA_03932 0.80 +0.5 773,936 + CCNA_03932 0.80 -0.9 773,944 - CCNA_03932 0.83 -1.6 773,944 - CCNA_03932 0.83 -4.0 773,944 - CCNA_03932 0.83 -3.5 773,946 + CCNA_03932 0.84 +0.5 773,946 + CCNA_03932 0.84 -0.6 773,974 - -0.9 774,001 + +0.2 774,057 + CCNA_03933 0.15 -0.8 774,057 + CCNA_03933 0.15 -1.5 774,060 + CCNA_03933 0.16 -1.9 774,090 - CCNA_03933 0.28 +0.1 774,090 - CCNA_03933 0.28 +0.7 774,101 + CCNA_03933 0.32 +0.9 774,107 + CCNA_03933 0.34 -2.6 774,109 - CCNA_03933 0.35 +0.2 774,119 + CCNA_03933 0.39 -2.3 774,120 - CCNA_03933 0.39 +0.2 774,124 + CCNA_03933 0.41 -2.4 774,124 + CCNA_03933 0.41 -1.5 774,134 + CCNA_03933 0.44 -0.5 774,148 + CCNA_03933 0.50 -0.8 774,188 + CCNA_03933 0.65 -1.6 774,192 + CCNA_03933 0.67 -1.3 774,207 + CCNA_03933 0.72 -1.3 774,214 + CCNA_03933 0.75 -1.1 774,285 + -2.5 774,285 + -0.7 774,293 + +0.5 774,293 - +0.4 774,293 - +0.6 774,293 - +0.4 774,405 + -1.0 774,448 + -1.9 774,467 + -1.0 774,475 - -1.4 774,511 + -0.3 774,516 + -0.9 774,535 - CCNA_00719 0.11 -1.3 774,596 - CCNA_00719 0.16 +0.7 774,626 - CCNA_00719 0.19 -0.9 774,685 + CCNA_00719 0.23 -0.1 774,703 + CCNA_00719 0.25 +1.3 774,743 + CCNA_00719 0.28 -0.8 774,743 + CCNA_00719 0.28 -1.5 774,743 + CCNA_00719 0.28 -0.5 774,756 + CCNA_00719 0.29 -2.1 774,764 - CCNA_00719 0.30 +1.5 774,838 + CCNA_00719 0.36 -0.1 774,838 + CCNA_00719 0.36 -0.1 774,838 + CCNA_00719 0.36 -0.8 774,838 + CCNA_00719 0.36 -1.1 774,838 + CCNA_00719 0.36 +0.5 774,838 + CCNA_00719 0.36 -0.5 774,838 + CCNA_00719 0.36 -1.1 774,845 + CCNA_00719 0.36 -1.5 774,845 + CCNA_00719 0.36 -1.7 774,940 + CCNA_00719 0.44 +1.1 774,940 + CCNA_00719 0.44 -1.1 774,948 - CCNA_00719 0.45 -0.7 774,975 - CCNA_00719 0.47 -1.7
Or see this region's nucleotide sequence