Experiment: Def Carbenicillin 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3815 and Psest_3816 are separated by 115 nucleotides Psest_3816 and Psest_3817 are separated by 60 nucleotides Psest_3817 and Psest_3818 are separated by 104 nucleotides Psest_3818 and Psest_3819 are separated by 43 nucleotides
Psest_3815: Psest_3815 - guanylate kinase, at 3,985,421 to 3,986,041
_3815
Psest_3816: Psest_3816 - DNA-directed RNA polymerase, omega subunit, at 3,986,157 to 3,986,420
_3816
Psest_3817: Psest_3817 - (p)ppGpp synthetase, RelA/SpoT family, at 3,986,481 to 3,988,589
_3817
Psest_3818: Psest_3818 - endoribonuclease L-PSP, putative, at 3,988,694 to 3,989,074
_3818
Psest_3819: Psest_3819 - Outer membrane protein and related peptidoglycan-associated (lipo)proteins, at 3,989,118 to 3,989,690
_3819
Position (kb)
3986
3987
3988
3989 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3986.040 kb on - strand at 3986.097 kb on + strand at 3986.102 kb on + strand at 3986.337 kb on + strand, within Psest_3816 at 3986.337 kb on + strand, within Psest_3816 at 3988.655 kb on - strand at 3988.745 kb on + strand, within Psest_3818 at 3988.745 kb on + strand, within Psest_3818 at 3988.746 kb on - strand, within Psest_3818 at 3988.746 kb on - strand, within Psest_3818 at 3988.746 kb on - strand, within Psest_3818 at 3988.746 kb on - strand, within Psest_3818 at 3988.746 kb on - strand, within Psest_3818 at 3988.771 kb on + strand, within Psest_3818 at 3988.778 kb on + strand, within Psest_3818 at 3988.778 kb on + strand, within Psest_3818 at 3988.778 kb on + strand, within Psest_3818 at 3988.778 kb on + strand, within Psest_3818 at 3988.779 kb on - strand, within Psest_3818 at 3988.779 kb on - strand, within Psest_3818 at 3988.779 kb on - strand, within Psest_3818 at 3989.083 kb on + strand at 3989.083 kb on + strand at 3989.084 kb on - strand at 3989.084 kb on - strand at 3989.144 kb on + strand at 3989.247 kb on + strand, within Psest_3819 at 3989.248 kb on - strand, within Psest_3819
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.02 mg/ml remove 3,986,040 - -0.4 3,986,097 + +1.9 3,986,102 + -0.3 3,986,337 + Psest_3816 0.68 -2.0 3,986,337 + Psest_3816 0.68 -0.3 3,988,655 - -1.5 3,988,745 + Psest_3818 0.13 -0.0 3,988,745 + Psest_3818 0.13 -2.0 3,988,746 - Psest_3818 0.14 -3.4 3,988,746 - Psest_3818 0.14 +0.9 3,988,746 - Psest_3818 0.14 +2.4 3,988,746 - Psest_3818 0.14 +2.6 3,988,746 - Psest_3818 0.14 -1.3 3,988,771 + Psest_3818 0.20 -3.2 3,988,778 + Psest_3818 0.22 -0.7 3,988,778 + Psest_3818 0.22 -2.3 3,988,778 + Psest_3818 0.22 -2.0 3,988,778 + Psest_3818 0.22 +0.6 3,988,779 - Psest_3818 0.22 +0.9 3,988,779 - Psest_3818 0.22 +2.3 3,988,779 - Psest_3818 0.22 -0.1 3,989,083 + -0.2 3,989,083 + -0.2 3,989,084 - -1.0 3,989,084 - -0.6 3,989,144 + -1.5 3,989,247 + Psest_3819 0.23 -1.3 3,989,248 - Psest_3819 0.23 -0.1
Or see this region's nucleotide sequence