Experiment: Def Carbenicillin 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0364 and Psest_0365 are separated by 41 nucleotides Psest_0365 and Psest_0366 are separated by 377 nucleotides Psest_0366 and Psest_0367 overlap by 11 nucleotides
Psest_0364: Psest_0364 - Gluconolactonase, at 390,412 to 391,302
_0364
Psest_0365: Psest_0365 - NAD dependent epimerase/dehydratase family., at 391,344 to 392,159
_0365
Psest_0366: Psest_0366 - Predicted membrane protein, at 392,537 to 392,845
_0366
Psest_0367: Psest_0367 - Predicted branched-chain amino acid permease (azaleucine resistance), at 392,835 to 393,551
_0367
Position (kb)
391
392
393 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 390.755 kb on + strand, within Psest_0364 at 390.803 kb on + strand, within Psest_0364 at 390.803 kb on + strand, within Psest_0364 at 390.803 kb on + strand, within Psest_0364 at 390.803 kb on + strand, within Psest_0364 at 390.803 kb on + strand, within Psest_0364 at 390.803 kb on + strand, within Psest_0364 at 390.804 kb on - strand, within Psest_0364 at 390.804 kb on - strand, within Psest_0364 at 390.804 kb on - strand, within Psest_0364 at 390.804 kb on - strand, within Psest_0364 at 390.804 kb on - strand, within Psest_0364 at 390.804 kb on - strand, within Psest_0364 at 390.804 kb on - strand, within Psest_0364 at 390.804 kb on - strand, within Psest_0364 at 390.804 kb on - strand, within Psest_0364 at 390.804 kb on - strand, within Psest_0364 at 391.217 kb on + strand at 391.323 kb on + strand at 391.324 kb on - strand at 391.324 kb on - strand at 391.384 kb on + strand at 391.384 kb on + strand at 391.385 kb on - strand at 391.385 kb on - strand at 391.385 kb on - strand at 391.385 kb on - strand at 391.385 kb on - strand at 391.385 kb on - strand at 391.411 kb on + strand at 391.412 kb on - strand at 391.528 kb on + strand, within Psest_0365 at 391.687 kb on + strand, within Psest_0365 at 391.687 kb on + strand, within Psest_0365 at 391.688 kb on - strand, within Psest_0365 at 391.688 kb on - strand, within Psest_0365 at 391.688 kb on - strand, within Psest_0365 at 391.690 kb on + strand, within Psest_0365 at 391.691 kb on - strand, within Psest_0365 at 391.691 kb on - strand, within Psest_0365 at 391.691 kb on - strand, within Psest_0365 at 391.691 kb on - strand, within Psest_0365 at 391.691 kb on - strand, within Psest_0365 at 391.691 kb on - strand, within Psest_0365 at 391.691 kb on - strand, within Psest_0365 at 391.732 kb on + strand, within Psest_0365 at 391.735 kb on + strand, within Psest_0365 at 391.736 kb on - strand, within Psest_0365 at 391.736 kb on - strand, within Psest_0365 at 391.855 kb on + strand, within Psest_0365 at 391.856 kb on - strand, within Psest_0365 at 391.856 kb on - strand, within Psest_0365 at 391.856 kb on - strand, within Psest_0365 at 391.859 kb on + strand, within Psest_0365 at 391.859 kb on + strand, within Psest_0365 at 391.860 kb on - strand, within Psest_0365 at 392.065 kb on + strand, within Psest_0365 at 392.154 kb on - strand at 392.215 kb on - strand at 392.272 kb on + strand at 392.272 kb on + strand at 392.272 kb on + strand at 392.272 kb on + strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.273 kb on - strand at 392.274 kb on + strand at 392.274 kb on + strand at 392.275 kb on - strand at 392.275 kb on - strand at 392.275 kb on - strand at 392.313 kb on + strand at 392.313 kb on + strand at 392.313 kb on + strand at 392.314 kb on - strand at 392.314 kb on - strand at 392.314 kb on - strand at 392.482 kb on - strand at 392.923 kb on + strand, within Psest_0367
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.02 mg/ml remove 390,755 + Psest_0364 0.38 -0.9 390,803 + Psest_0364 0.44 -1.3 390,803 + Psest_0364 0.44 +2.7 390,803 + Psest_0364 0.44 +1.6 390,803 + Psest_0364 0.44 -1.3 390,803 + Psest_0364 0.44 -2.4 390,803 + Psest_0364 0.44 -3.0 390,804 - Psest_0364 0.44 +0.5 390,804 - Psest_0364 0.44 +2.7 390,804 - Psest_0364 0.44 -0.3 390,804 - Psest_0364 0.44 +0.8 390,804 - Psest_0364 0.44 -0.8 390,804 - Psest_0364 0.44 -1.3 390,804 - Psest_0364 0.44 +2.4 390,804 - Psest_0364 0.44 -1.7 390,804 - Psest_0364 0.44 +0.2 390,804 - Psest_0364 0.44 +0.1 391,217 + -1.5 391,323 + +1.1 391,324 - +1.0 391,324 - +2.9 391,384 + -0.6 391,384 + +1.4 391,385 - -2.5 391,385 - -0.5 391,385 - +1.9 391,385 - -1.0 391,385 - -1.5 391,385 - +0.1 391,411 + -0.1 391,412 - +0.8 391,528 + Psest_0365 0.23 +0.3 391,687 + Psest_0365 0.42 +0.6 391,687 + Psest_0365 0.42 +1.4 391,688 - Psest_0365 0.42 +0.0 391,688 - Psest_0365 0.42 -1.2 391,688 - Psest_0365 0.42 -3.1 391,690 + Psest_0365 0.42 +1.1 391,691 - Psest_0365 0.43 +0.5 391,691 - Psest_0365 0.43 +2.3 391,691 - Psest_0365 0.43 +1.0 391,691 - Psest_0365 0.43 +1.8 391,691 - Psest_0365 0.43 -0.7 391,691 - Psest_0365 0.43 +0.4 391,691 - Psest_0365 0.43 -2.0 391,732 + Psest_0365 0.48 -0.3 391,735 + Psest_0365 0.48 +0.6 391,736 - Psest_0365 0.48 +1.3 391,736 - Psest_0365 0.48 -0.9 391,855 + Psest_0365 0.63 -1.5 391,856 - Psest_0365 0.63 +0.1 391,856 - Psest_0365 0.63 +0.5 391,856 - Psest_0365 0.63 +0.5 391,859 + Psest_0365 0.63 -0.4 391,859 + Psest_0365 0.63 -0.4 391,860 - Psest_0365 0.63 +2.1 392,065 + Psest_0365 0.88 +1.3 392,154 - -0.1 392,215 - +0.9 392,272 + -0.1 392,272 + -0.4 392,272 + +0.6 392,272 + -1.4 392,273 - -1.0 392,273 - -0.9 392,273 - -0.6 392,273 - -0.6 392,273 - +1.1 392,273 - -0.1 392,273 - +1.1 392,273 - +2.2 392,273 - +4.2 392,273 - +1.2 392,273 - +1.7 392,273 - -0.7 392,273 - +1.1 392,273 - +0.5 392,273 - -0.4 392,273 - -2.7 392,273 - +1.3 392,273 - +1.2 392,273 - +2.2 392,273 - +1.8 392,274 + +0.5 392,274 + -0.1 392,275 - -1.0 392,275 - +1.2 392,275 - +4.6 392,313 + -0.7 392,313 + -0.9 392,313 + +0.6 392,314 - +1.0 392,314 - +1.9 392,314 - -0.1 392,482 - -2.1 392,923 + Psest_0367 0.12 +0.9
Or see this region's nucleotide sequence