Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_3548

Experiment: Def Carbenicillin 0.02 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_3547 and Psest_3548 are separated by 12 nucleotidesPsest_3548 and Psest_3549 overlap by 4 nucleotidesPsest_3549 and Psest_3550 are separated by 59 nucleotides Psest_3547: Psest_3547 - thiamine-monophosphate kinase, at 3,726,771 to 3,727,733 _3547 Psest_3548: Psest_3548 - transcription antitermination factor NusB, at 3,727,746 to 3,728,231 _3548 Psest_3549: Psest_3549 - 6,7-dimethyl-8-ribityllumazine synthase, at 3,728,228 to 3,728,704 _3549 Psest_3550: Psest_3550 - 3,4-dihydroxy-2-butanone 4-phosphate synthase, at 3,728,764 to 3,729,843 _3550 Position (kb) 3727 3728 3729Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 3728.781 kb on - strandat 3728.781 kb on - strandat 3728.786 kb on - strandat 3728.786 kb on - strandat 3728.786 kb on - strandat 3728.786 kb on - strandat 3729.044 kb on - strand, within Psest_3550at 3729.172 kb on - strand, within Psest_3550at 3729.172 kb on - strand, within Psest_3550at 3729.172 kb on - strand, within Psest_3550

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Carbenicillin 0.02 mg/ml
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3,728,781 - -1.9
3,728,781 - -1.2
3,728,786 - +0.5
3,728,786 - +5.6
3,728,786 - -1.1
3,728,786 - +0.8
3,729,044 - Psest_3550 0.26 +0.4
3,729,172 - Psest_3550 0.38 +0.1
3,729,172 - Psest_3550 0.38 -0.1
3,729,172 - Psest_3550 0.38 -0.2

Or see this region's nucleotide sequence