Experiment: Def Carbenicillin 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0262 and Psest_0263 are separated by 185 nucleotides Psest_0263 and Psest_0264 are separated by 120 nucleotides Psest_0264 and Psest_0265 are separated by 68 nucleotides
Psest_0262: Psest_0262 - PAS domain S-box, at 276,594 to 279,164
_0262
Psest_0263: Psest_0263 - PAS domain S-box/diguanylate cyclase (GGDEF) domain, at 279,350 to 281,278
_0263
Psest_0264: Psest_0264 - monofunctional biosynthetic peptidoglycan transglycosylase, at 281,399 to 282,127
_0264
Psest_0265: Psest_0265 - Uncharacterized small membrane protein, at 282,196 to 282,570
_0265
Position (kb) 279
280
281
282 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 278.374 kb on + strand, within Psest_0262 at 278.374 kb on + strand, within Psest_0262 at 278.374 kb on + strand, within Psest_0262 at 278.374 kb on + strand, within Psest_0262 at 278.374 kb on + strand, within Psest_0262 at 278.374 kb on + strand, within Psest_0262 at 278.374 kb on + strand, within Psest_0262 at 278.374 kb on + strand, within Psest_0262 at 278.374 kb on + strand, within Psest_0262 at 278.375 kb on - strand, within Psest_0262 at 278.375 kb on - strand, within Psest_0262 at 278.375 kb on - strand, within Psest_0262 at 278.375 kb on - strand, within Psest_0262 at 278.375 kb on - strand, within Psest_0262 at 278.375 kb on - strand, within Psest_0262 at 278.375 kb on - strand, within Psest_0262 at 278.375 kb on - strand, within Psest_0262 at 278.375 kb on - strand, within Psest_0262 at 278.375 kb on - strand, within Psest_0262 at 278.375 kb on - strand, within Psest_0262 at 278.375 kb on - strand, within Psest_0262 at 278.375 kb on - strand, within Psest_0262 at 278.375 kb on - strand, within Psest_0262 at 278.375 kb on - strand, within Psest_0262 at 278.378 kb on + strand, within Psest_0262 at 278.378 kb on + strand, within Psest_0262 at 278.378 kb on + strand, within Psest_0262 at 278.378 kb on + strand, within Psest_0262 at 278.378 kb on + strand, within Psest_0262 at 278.378 kb on + strand, within Psest_0262 at 278.378 kb on + strand, within Psest_0262 at 278.378 kb on + strand, within Psest_0262 at 278.378 kb on + strand, within Psest_0262 at 278.378 kb on + strand, within Psest_0262 at 278.378 kb on + strand, within Psest_0262 at 278.378 kb on + strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.379 kb on - strand, within Psest_0262 at 278.450 kb on - strand, within Psest_0262 at 278.450 kb on - strand, within Psest_0262 at 278.450 kb on - strand, within Psest_0262 at 278.512 kb on + strand, within Psest_0262 at 278.520 kb on + strand, within Psest_0262 at 278.521 kb on - strand, within Psest_0262 at 278.524 kb on + strand, within Psest_0262 at 278.524 kb on + strand, within Psest_0262 at 278.524 kb on + strand, within Psest_0262 at 278.524 kb on + strand, within Psest_0262 at 278.524 kb on + strand, within Psest_0262 at 278.524 kb on + strand, within Psest_0262 at 278.524 kb on + strand, within Psest_0262 at 278.525 kb on - strand, within Psest_0262 at 278.525 kb on - strand, within Psest_0262 at 278.525 kb on - strand, within Psest_0262 at 278.525 kb on - strand, within Psest_0262 at 278.525 kb on - strand, within Psest_0262 at 278.525 kb on - strand, within Psest_0262 at 278.567 kb on + strand, within Psest_0262 at 278.568 kb on - strand, within Psest_0262 at 278.647 kb on + strand, within Psest_0262 at 278.648 kb on - strand, within Psest_0262 at 278.648 kb on - strand, within Psest_0262 at 278.648 kb on - strand, within Psest_0262 at 278.648 kb on - strand, within Psest_0262 at 278.648 kb on - strand, within Psest_0262 at 278.648 kb on - strand, within Psest_0262 at 278.648 kb on - strand, within Psest_0262 at 278.711 kb on + strand, within Psest_0262 at 278.711 kb on + strand, within Psest_0262 at 278.711 kb on + strand, within Psest_0262 at 278.712 kb on - strand, within Psest_0262 at 278.713 kb on + strand, within Psest_0262 at 278.714 kb on - strand, within Psest_0262 at 278.714 kb on - strand, within Psest_0262 at 278.714 kb on - strand, within Psest_0262 at 278.714 kb on - strand, within Psest_0262 at 278.714 kb on - strand, within Psest_0262 at 278.872 kb on + strand, within Psest_0262 at 278.872 kb on + strand, within Psest_0262 at 278.872 kb on + strand, within Psest_0262 at 278.872 kb on + strand, within Psest_0262 at 278.872 kb on + strand, within Psest_0262 at 278.873 kb on - strand, within Psest_0262 at 278.873 kb on - strand, within Psest_0262 at 278.873 kb on - strand, within Psest_0262 at 278.873 kb on - strand, within Psest_0262 at 278.873 kb on - strand, within Psest_0262 at 278.873 kb on - strand, within Psest_0262 at 278.873 kb on - strand, within Psest_0262 at 278.873 kb on - strand, within Psest_0262 at 279.075 kb on + strand at 279.248 kb on + strand at 279.249 kb on - strand at 279.249 kb on - strand at 279.314 kb on + strand at 279.314 kb on + strand at 279.315 kb on - strand at 279.315 kb on - strand at 279.515 kb on + strand at 279.515 kb on + strand at 279.515 kb on + strand at 279.515 kb on + strand at 279.515 kb on + strand at 279.516 kb on - strand at 279.516 kb on - strand at 279.516 kb on - strand at 279.622 kb on + strand, within Psest_0263 at 279.622 kb on + strand, within Psest_0263 at 279.622 kb on + strand, within Psest_0263 at 279.623 kb on - strand, within Psest_0263 at 279.812 kb on - strand, within Psest_0263 at 279.958 kb on + strand, within Psest_0263 at 279.958 kb on + strand, within Psest_0263 at 279.958 kb on + strand, within Psest_0263 at 279.958 kb on + strand, within Psest_0263 at 280.074 kb on + strand, within Psest_0263 at 280.075 kb on - strand, within Psest_0263 at 280.075 kb on - strand, within Psest_0263 at 280.075 kb on - strand, within Psest_0263 at 280.086 kb on + strand, within Psest_0263 at 280.179 kb on + strand, within Psest_0263 at 280.180 kb on - strand, within Psest_0263 at 280.188 kb on + strand, within Psest_0263 at 280.188 kb on + strand, within Psest_0263 at 280.188 kb on + strand, within Psest_0263 at 280.188 kb on + strand, within Psest_0263 at 280.188 kb on + strand, within Psest_0263 at 280.188 kb on + strand, within Psest_0263 at 280.189 kb on - strand, within Psest_0263 at 280.189 kb on - strand, within Psest_0263 at 280.282 kb on - strand, within Psest_0263 at 280.660 kb on - strand, within Psest_0263 at 280.660 kb on - strand, within Psest_0263 at 280.728 kb on + strand, within Psest_0263 at 280.729 kb on - strand, within Psest_0263 at 280.866 kb on + strand, within Psest_0263 at 280.867 kb on - strand, within Psest_0263 at 280.867 kb on - strand, within Psest_0263 at 280.946 kb on - strand, within Psest_0263 at 281.118 kb on - strand at 281.244 kb on + strand at 281.245 kb on - strand at 281.268 kb on + strand at 281.268 kb on + strand at 281.268 kb on + strand at 281.268 kb on + strand at 281.268 kb on + strand at 281.268 kb on + strand at 281.268 kb on + strand at 281.268 kb on + strand at 281.268 kb on + strand at 281.268 kb on + strand at 281.268 kb on + strand at 281.269 kb on - strand at 281.269 kb on - strand at 281.269 kb on - strand at 281.269 kb on - strand at 281.269 kb on - strand at 281.269 kb on - strand at 281.269 kb on - strand at 281.269 kb on - strand at 281.303 kb on - strand at 281.344 kb on - strand at 281.390 kb on + strand at 281.390 kb on + strand at 281.390 kb on + strand at 281.390 kb on + strand at 281.390 kb on + strand at 281.390 kb on + strand at 281.390 kb on + strand at 281.391 kb on - strand at 281.391 kb on - strand at 281.391 kb on - strand at 281.391 kb on - strand at 281.400 kb on + strand at 281.400 kb on + strand at 281.400 kb on + strand at 281.400 kb on + strand at 281.401 kb on - strand at 281.451 kb on + strand at 281.452 kb on - strand at 281.511 kb on + strand, within Psest_0264 at 281.511 kb on + strand, within Psest_0264 at 281.511 kb on + strand, within Psest_0264 at 281.511 kb on + strand, within Psest_0264 at 281.511 kb on + strand, within Psest_0264 at 281.511 kb on + strand, within Psest_0264 at 281.511 kb on + strand, within Psest_0264 at 281.511 kb on + strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.512 kb on - strand, within Psest_0264 at 281.556 kb on - strand, within Psest_0264 at 281.673 kb on + strand, within Psest_0264 at 281.673 kb on + strand, within Psest_0264 at 281.673 kb on + strand, within Psest_0264 at 281.673 kb on + strand, within Psest_0264 at 281.674 kb on - strand, within Psest_0264 at 281.674 kb on - strand, within Psest_0264 at 281.674 kb on - strand, within Psest_0264 at 281.674 kb on - strand, within Psest_0264 at 281.674 kb on - strand, within Psest_0264 at 281.674 kb on - strand, within Psest_0264 at 281.674 kb on - strand, within Psest_0264 at 281.674 kb on - strand, within Psest_0264 at 281.974 kb on + strand, within Psest_0264 at 282.056 kb on + strand at 282.056 kb on + strand at 282.057 kb on - strand at 282.057 kb on - strand at 282.160 kb on + strand at 282.160 kb on + strand at 282.160 kb on + strand at 282.161 kb on - strand at 282.161 kb on - strand at 282.161 kb on - strand at 282.161 kb on - strand at 282.161 kb on - strand at 282.162 kb on + strand at 282.163 kb on - strand at 282.208 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.02 mg/ml remove 278,374 + Psest_0262 0.69 -0.3 278,374 + Psest_0262 0.69 -0.1 278,374 + Psest_0262 0.69 -2.8 278,374 + Psest_0262 0.69 +0.7 278,374 + Psest_0262 0.69 +3.2 278,374 + Psest_0262 0.69 -2.9 278,374 + Psest_0262 0.69 +1.8 278,374 + Psest_0262 0.69 +1.4 278,374 + Psest_0262 0.69 -1.1 278,375 - Psest_0262 0.69 -1.5 278,375 - Psest_0262 0.69 -1.0 278,375 - Psest_0262 0.69 +0.4 278,375 - Psest_0262 0.69 +0.3 278,375 - Psest_0262 0.69 -0.3 278,375 - Psest_0262 0.69 -4.0 278,375 - Psest_0262 0.69 -1.7 278,375 - Psest_0262 0.69 +1.3 278,375 - Psest_0262 0.69 -1.5 278,375 - Psest_0262 0.69 -2.7 278,375 - Psest_0262 0.69 +0.5 278,375 - Psest_0262 0.69 -0.2 278,375 - Psest_0262 0.69 +0.5 278,375 - Psest_0262 0.69 +0.5 278,375 - Psest_0262 0.69 -0.7 278,378 + Psest_0262 0.69 +0.4 278,378 + Psest_0262 0.69 -0.9 278,378 + Psest_0262 0.69 -0.9 278,378 + Psest_0262 0.69 +0.2 278,378 + Psest_0262 0.69 +0.1 278,378 + Psest_0262 0.69 -1.3 278,378 + Psest_0262 0.69 +0.8 278,378 + Psest_0262 0.69 -1.8 278,378 + Psest_0262 0.69 +0.9 278,378 + Psest_0262 0.69 -0.5 278,378 + Psest_0262 0.69 +0.7 278,378 + Psest_0262 0.69 -0.7 278,379 - Psest_0262 0.69 -1.0 278,379 - Psest_0262 0.69 -2.0 278,379 - Psest_0262 0.69 +1.2 278,379 - Psest_0262 0.69 +1.1 278,379 - Psest_0262 0.69 +0.8 278,379 - Psest_0262 0.69 -2.4 278,379 - Psest_0262 0.69 -1.5 278,379 - Psest_0262 0.69 +0.8 278,379 - Psest_0262 0.69 -0.7 278,379 - Psest_0262 0.69 -0.0 278,379 - Psest_0262 0.69 -0.7 278,379 - Psest_0262 0.69 -1.3 278,379 - Psest_0262 0.69 +2.2 278,379 - Psest_0262 0.69 -2.4 278,379 - Psest_0262 0.69 -0.8 278,379 - Psest_0262 0.69 -0.4 278,379 - Psest_0262 0.69 -2.5 278,379 - Psest_0262 0.69 -0.9 278,379 - Psest_0262 0.69 -3.5 278,379 - Psest_0262 0.69 -0.2 278,379 - Psest_0262 0.69 -0.7 278,379 - Psest_0262 0.69 -0.2 278,450 - Psest_0262 0.72 -0.4 278,450 - Psest_0262 0.72 +1.8 278,450 - Psest_0262 0.72 -1.1 278,512 + Psest_0262 0.75 -1.0 278,520 + Psest_0262 0.75 -0.2 278,521 - Psest_0262 0.75 -0.3 278,524 + Psest_0262 0.75 -0.2 278,524 + Psest_0262 0.75 +1.2 278,524 + Psest_0262 0.75 -3.7 278,524 + Psest_0262 0.75 -0.7 278,524 + Psest_0262 0.75 +1.2 278,524 + Psest_0262 0.75 +0.2 278,524 + Psest_0262 0.75 -0.4 278,525 - Psest_0262 0.75 +0.4 278,525 - Psest_0262 0.75 -2.1 278,525 - Psest_0262 0.75 -0.8 278,525 - Psest_0262 0.75 -2.4 278,525 - Psest_0262 0.75 -2.8 278,525 - Psest_0262 0.75 +4.1 278,567 + Psest_0262 0.77 +0.0 278,568 - Psest_0262 0.77 +0.1 278,647 + Psest_0262 0.80 -2.3 278,648 - Psest_0262 0.80 +0.5 278,648 - Psest_0262 0.80 +1.1 278,648 - Psest_0262 0.80 -1.1 278,648 - Psest_0262 0.80 +0.5 278,648 - Psest_0262 0.80 -1.3 278,648 - Psest_0262 0.80 +1.3 278,648 - Psest_0262 0.80 +1.0 278,711 + Psest_0262 0.82 +0.4 278,711 + Psest_0262 0.82 +1.5 278,711 + Psest_0262 0.82 -3.6 278,712 - Psest_0262 0.82 +1.7 278,713 + Psest_0262 0.82 -0.6 278,714 - Psest_0262 0.82 -2.9 278,714 - Psest_0262 0.82 -3.2 278,714 - Psest_0262 0.82 -1.6 278,714 - Psest_0262 0.82 -0.4 278,714 - Psest_0262 0.82 +1.3 278,872 + Psest_0262 0.89 -0.4 278,872 + Psest_0262 0.89 +0.6 278,872 + Psest_0262 0.89 +0.4 278,872 + Psest_0262 0.89 +1.4 278,872 + Psest_0262 0.89 -3.1 278,873 - Psest_0262 0.89 -1.7 278,873 - Psest_0262 0.89 +5.2 278,873 - Psest_0262 0.89 -0.9 278,873 - Psest_0262 0.89 +0.2 278,873 - Psest_0262 0.89 -1.6 278,873 - Psest_0262 0.89 -0.9 278,873 - Psest_0262 0.89 +1.3 278,873 - Psest_0262 0.89 -2.6 279,075 + -0.9 279,248 + -1.1 279,249 - +0.5 279,249 - +0.3 279,314 + -0.0 279,314 + -2.3 279,315 - +1.5 279,315 - +0.7 279,515 + -1.9 279,515 + -1.5 279,515 + -1.3 279,515 + -1.2 279,515 + +0.1 279,516 - -1.1 279,516 - -4.1 279,516 - -2.3 279,622 + Psest_0263 0.14 +0.2 279,622 + Psest_0263 0.14 +0.5 279,622 + Psest_0263 0.14 -2.0 279,623 - Psest_0263 0.14 -1.2 279,812 - Psest_0263 0.24 -1.2 279,958 + Psest_0263 0.32 -2.6 279,958 + Psest_0263 0.32 -0.4 279,958 + Psest_0263 0.32 -2.2 279,958 + Psest_0263 0.32 -2.3 280,074 + Psest_0263 0.38 -0.1 280,075 - Psest_0263 0.38 -3.1 280,075 - Psest_0263 0.38 -2.4 280,075 - Psest_0263 0.38 -0.9 280,086 + Psest_0263 0.38 -1.8 280,179 + Psest_0263 0.43 +0.1 280,180 - Psest_0263 0.43 +0.7 280,188 + Psest_0263 0.43 +0.0 280,188 + Psest_0263 0.43 -3.5 280,188 + Psest_0263 0.43 +1.2 280,188 + Psest_0263 0.43 -0.4 280,188 + Psest_0263 0.43 -0.1 280,188 + Psest_0263 0.43 +0.7 280,189 - Psest_0263 0.43 +1.3 280,189 - Psest_0263 0.43 -0.1 280,282 - Psest_0263 0.48 +0.5 280,660 - Psest_0263 0.68 +0.1 280,660 - Psest_0263 0.68 -1.3 280,728 + Psest_0263 0.71 -2.2 280,729 - Psest_0263 0.71 -1.9 280,866 + Psest_0263 0.79 +0.3 280,867 - Psest_0263 0.79 +0.6 280,867 - Psest_0263 0.79 -1.6 280,946 - Psest_0263 0.83 -0.8 281,118 - +1.2 281,244 + -1.5 281,245 - -0.6 281,268 + -2.3 281,268 + +1.4 281,268 + -1.6 281,268 + -0.1 281,268 + -1.7 281,268 + -3.6 281,268 + -3.1 281,268 + +0.2 281,268 + -0.1 281,268 + -1.0 281,268 + +0.3 281,269 - +0.3 281,269 - +0.6 281,269 - +0.6 281,269 - +0.4 281,269 - -0.9 281,269 - -2.3 281,269 - -3.9 281,269 - -1.2 281,303 - -0.3 281,344 - -0.4 281,390 + -0.5 281,390 + +0.0 281,390 + +1.4 281,390 + -1.4 281,390 + -1.7 281,390 + -0.4 281,390 + -0.6 281,391 - -0.1 281,391 - +1.0 281,391 - +0.0 281,391 - +1.9 281,400 + +1.2 281,400 + -0.1 281,400 + -1.9 281,400 + +1.9 281,401 - +0.6 281,451 + +0.1 281,452 - -2.2 281,511 + Psest_0264 0.15 -2.5 281,511 + Psest_0264 0.15 +1.0 281,511 + Psest_0264 0.15 +0.1 281,511 + Psest_0264 0.15 -3.0 281,511 + Psest_0264 0.15 -0.9 281,511 + Psest_0264 0.15 -0.7 281,511 + Psest_0264 0.15 -0.8 281,511 + Psest_0264 0.15 -1.9 281,512 - Psest_0264 0.16 -1.9 281,512 - Psest_0264 0.16 +1.1 281,512 - Psest_0264 0.16 -0.2 281,512 - Psest_0264 0.16 +0.6 281,512 - Psest_0264 0.16 +2.0 281,512 - Psest_0264 0.16 +0.6 281,512 - Psest_0264 0.16 +0.3 281,512 - Psest_0264 0.16 +1.8 281,512 - Psest_0264 0.16 +1.1 281,512 - Psest_0264 0.16 +1.0 281,512 - Psest_0264 0.16 +0.2 281,512 - Psest_0264 0.16 +1.8 281,512 - Psest_0264 0.16 +0.6 281,512 - Psest_0264 0.16 -0.8 281,512 - Psest_0264 0.16 -0.8 281,512 - Psest_0264 0.16 +0.6 281,512 - Psest_0264 0.16 -0.2 281,512 - Psest_0264 0.16 +0.3 281,512 - Psest_0264 0.16 -1.1 281,512 - Psest_0264 0.16 +0.9 281,512 - Psest_0264 0.16 +0.9 281,512 - Psest_0264 0.16 -0.9 281,512 - Psest_0264 0.16 +0.9 281,512 - Psest_0264 0.16 +0.1 281,512 - Psest_0264 0.16 +0.2 281,512 - Psest_0264 0.16 +0.9 281,512 - Psest_0264 0.16 +1.1 281,556 - Psest_0264 0.22 +1.1 281,673 + Psest_0264 0.38 -1.4 281,673 + Psest_0264 0.38 +0.6 281,673 + Psest_0264 0.38 -0.9 281,673 + Psest_0264 0.38 -1.9 281,674 - Psest_0264 0.38 +0.2 281,674 - Psest_0264 0.38 -3.2 281,674 - Psest_0264 0.38 -2.0 281,674 - Psest_0264 0.38 +0.6 281,674 - Psest_0264 0.38 -1.9 281,674 - Psest_0264 0.38 +0.8 281,674 - Psest_0264 0.38 +1.4 281,674 - Psest_0264 0.38 -0.5 281,974 + Psest_0264 0.79 -1.7 282,056 + -0.2 282,056 + -1.9 282,057 - +0.0 282,057 - -0.2 282,160 + +0.6 282,160 + -2.9 282,160 + -0.1 282,161 - -0.4 282,161 - +0.6 282,161 - -0.4 282,161 - -0.7 282,161 - -0.3 282,162 + +0.2 282,163 - +0.1 282,208 + -0.6
Or see this region's nucleotide sequence