Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0024

Experiment: Def Carbenicillin 0.02 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0022 and Psest_0023 are separated by 55 nucleotidesPsest_0023 and Psest_0024 are separated by 148 nucleotidesPsest_0024 and Psest_0025 are separated by 87 nucleotides Psest_0022: Psest_0022 - methionyl-tRNA formyltransferase, at 23,022 to 23,966 _0022 Psest_0023: Psest_0023 - peptide deformylase, at 24,022 to 24,528 _0023 Psest_0024: Psest_0024 - LysM domain., at 24,677 to 25,702 _0024 Psest_0025: Psest_0025 - DNA protecting protein DprA, at 25,790 to 26,887 _0025 Position (kb) 24 25 26Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 24.529 kb on - strandat 24.529 kb on - strandat 24.555 kb on - strandat 24.555 kb on - strandat 24.574 kb on - strandat 24.574 kb on - strandat 24.574 kb on - strandat 24.659 kb on - strandat 24.659 kb on - strandat 24.659 kb on - strandat 24.659 kb on - strandat 24.659 kb on - strandat 24.662 kb on - strandat 24.662 kb on - strandat 24.662 kb on - strandat 24.768 kb on - strandat 24.769 kb on + strandat 24.769 kb on + strandat 24.769 kb on + strandat 24.769 kb on + strandat 24.884 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.936 kb on + strand, within Psest_0024at 24.937 kb on - strand, within Psest_0024at 24.988 kb on + strand, within Psest_0024at 24.988 kb on + strand, within Psest_0024at 25.097 kb on + strand, within Psest_0024at 25.097 kb on + strand, within Psest_0024at 25.097 kb on + strand, within Psest_0024at 25.097 kb on + strand, within Psest_0024at 25.097 kb on + strand, within Psest_0024at 25.098 kb on - strand, within Psest_0024at 25.098 kb on - strand, within Psest_0024at 25.151 kb on + strand, within Psest_0024at 25.151 kb on + strand, within Psest_0024at 25.152 kb on - strand, within Psest_0024at 25.152 kb on - strand, within Psest_0024at 25.214 kb on + strand, within Psest_0024at 25.214 kb on + strand, within Psest_0024at 25.214 kb on + strand, within Psest_0024at 25.214 kb on + strand, within Psest_0024at 25.215 kb on - strand, within Psest_0024at 25.215 kb on - strand, within Psest_0024at 25.215 kb on - strand, within Psest_0024at 25.215 kb on - strand, within Psest_0024at 25.215 kb on - strand, within Psest_0024at 25.215 kb on - strand, within Psest_0024at 25.215 kb on - strand, within Psest_0024at 25.532 kb on + strand, within Psest_0024at 25.532 kb on + strand, within Psest_0024at 25.533 kb on - strand, within Psest_0024at 25.625 kb on + strandat 25.625 kb on + strandat 25.625 kb on + strandat 25.625 kb on + strandat 25.640 kb on + strandat 25.640 kb on + strandat 25.641 kb on - strandat 25.766 kb on + strandat 25.766 kb on + strandat 25.767 kb on - strandat 25.767 kb on - strandat 25.781 kb on + strandat 26.060 kb on + strand, within Psest_0025at 26.434 kb on + strand, within Psest_0025at 26.435 kb on - strand, within Psest_0025at 26.558 kb on + strand, within Psest_0025at 26.558 kb on + strand, within Psest_0025at 26.558 kb on + strand, within Psest_0025at 26.558 kb on + strand, within Psest_0025at 26.558 kb on + strand, within Psest_0025at 26.558 kb on + strand, within Psest_0025at 26.558 kb on + strand, within Psest_0025at 26.559 kb on - strand, within Psest_0025at 26.559 kb on - strand, within Psest_0025at 26.559 kb on - strand, within Psest_0025at 26.559 kb on - strand, within Psest_0025at 26.559 kb on - strand, within Psest_0025

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Carbenicillin 0.02 mg/ml
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24,529 - -1.2
24,529 - -0.8
24,555 - -1.5
24,555 - -1.5
24,574 - -2.5
24,574 - -3.1
24,574 - +0.4
24,659 - -0.8
24,659 - +1.7
24,659 - +0.5
24,659 - -1.5
24,659 - +1.4
24,662 - +0.9
24,662 - -0.4
24,662 - -1.0
24,768 - -0.6
24,769 + +0.3
24,769 + -1.6
24,769 + -1.1
24,769 + +0.0
24,884 + Psest_0024 0.20 +2.9
24,911 + Psest_0024 0.23 +1.1
24,911 + Psest_0024 0.23 +1.4
24,911 + Psest_0024 0.23 +0.4
24,911 + Psest_0024 0.23 -1.6
24,911 + Psest_0024 0.23 +0.2
24,911 + Psest_0024 0.23 +2.3
24,911 + Psest_0024 0.23 -1.7
24,911 + Psest_0024 0.23 +0.6
24,911 + Psest_0024 0.23 +0.9
24,911 + Psest_0024 0.23 +2.6
24,911 + Psest_0024 0.23 -1.9
24,912 - Psest_0024 0.23 +0.2
24,912 - Psest_0024 0.23 +0.2
24,912 - Psest_0024 0.23 +1.7
24,912 - Psest_0024 0.23 -0.7
24,912 - Psest_0024 0.23 +0.1
24,912 - Psest_0024 0.23 +0.3
24,912 - Psest_0024 0.23 -0.3
24,912 - Psest_0024 0.23 -0.2
24,912 - Psest_0024 0.23 +0.3
24,912 - Psest_0024 0.23 +2.3
24,912 - Psest_0024 0.23 -1.4
24,912 - Psest_0024 0.23 +1.4
24,936 + Psest_0024 0.25 +0.1
24,937 - Psest_0024 0.25 +2.4
24,988 + Psest_0024 0.30 -0.1
24,988 + Psest_0024 0.30 +1.6
25,097 + Psest_0024 0.41 +1.2
25,097 + Psest_0024 0.41 +0.0
25,097 + Psest_0024 0.41 -0.9
25,097 + Psest_0024 0.41 -0.1
25,097 + Psest_0024 0.41 -1.4
25,098 - Psest_0024 0.41 +0.2
25,098 - Psest_0024 0.41 +0.0
25,151 + Psest_0024 0.46 -1.4
25,151 + Psest_0024 0.46 +2.0
25,152 - Psest_0024 0.46 -2.6
25,152 - Psest_0024 0.46 -0.2
25,214 + Psest_0024 0.52 -0.4
25,214 + Psest_0024 0.52 -0.6
25,214 + Psest_0024 0.52 -0.6
25,214 + Psest_0024 0.52 +0.9
25,215 - Psest_0024 0.52 +2.3
25,215 - Psest_0024 0.52 -2.1
25,215 - Psest_0024 0.52 -0.7
25,215 - Psest_0024 0.52 +1.6
25,215 - Psest_0024 0.52 +0.1
25,215 - Psest_0024 0.52 -2.0
25,215 - Psest_0024 0.52 +1.2
25,532 + Psest_0024 0.83 -0.9
25,532 + Psest_0024 0.83 +0.8
25,533 - Psest_0024 0.83 +0.7
25,625 + +2.0
25,625 + +0.2
25,625 + +0.6
25,625 + -0.3
25,640 + +0.0
25,640 + +1.0
25,641 - +1.5
25,766 + +0.0
25,766 + -0.1
25,767 - +2.7
25,767 - -0.2
25,781 + +0.8
26,060 + Psest_0025 0.25 +0.0
26,434 + Psest_0025 0.59 -1.0
26,435 - Psest_0025 0.59 +1.1
26,558 + Psest_0025 0.70 -1.1
26,558 + Psest_0025 0.70 -0.1
26,558 + Psest_0025 0.70 +1.9
26,558 + Psest_0025 0.70 +1.0
26,558 + Psest_0025 0.70 +2.0
26,558 + Psest_0025 0.70 -0.5
26,558 + Psest_0025 0.70 +1.4
26,559 - Psest_0025 0.70 +1.4
26,559 - Psest_0025 0.70 +0.4
26,559 - Psest_0025 0.70 +1.1
26,559 - Psest_0025 0.70 +1.3
26,559 - Psest_0025 0.70 +1.2

Or see this region's nucleotide sequence