Experiment: Def Carbenicillin 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2418 and Psest_2419 are separated by 7 nucleotides Psest_2419 and Psest_2420 are separated by 45 nucleotides
Psest_2418: Psest_2418 - diguanylate cyclase (GGDEF) domain, at 2,580,774 to 2,581,688
_2418
Psest_2419: Psest_2419 - AraC-type DNA-binding domain-containing proteins, at 2,581,696 to 2,582,733
_2419
Psest_2420: Psest_2420 - Transcriptional regulator, at 2,582,779 to 2,583,666
_2420
Position (kb)
2581
2582
2583 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2581.408 kb on + strand, within Psest_2418 at 2581.717 kb on + strand at 2581.831 kb on + strand, within Psest_2419 at 2581.831 kb on + strand, within Psest_2419 at 2581.832 kb on - strand, within Psest_2419 at 2581.832 kb on - strand, within Psest_2419 at 2582.099 kb on - strand, within Psest_2419 at 2582.099 kb on - strand, within Psest_2419 at 2582.099 kb on - strand, within Psest_2419 at 2582.252 kb on - strand, within Psest_2419 at 2582.321 kb on - strand, within Psest_2419 at 2582.435 kb on - strand, within Psest_2419 at 2582.435 kb on - strand, within Psest_2419 at 2582.435 kb on - strand, within Psest_2419 at 2582.435 kb on - strand, within Psest_2419 at 2582.441 kb on - strand, within Psest_2419 at 2582.590 kb on + strand, within Psest_2419 at 2582.591 kb on - strand, within Psest_2419 at 2582.596 kb on + strand, within Psest_2419 at 2582.596 kb on + strand, within Psest_2419 at 2582.743 kb on - strand at 2582.743 kb on - strand at 2582.743 kb on - strand at 2582.760 kb on - strand at 2583.214 kb on + strand, within Psest_2420 at 2583.215 kb on - strand, within Psest_2420 at 2583.215 kb on - strand, within Psest_2420 at 2583.351 kb on - strand, within Psest_2420 at 2583.449 kb on + strand, within Psest_2420 at 2583.449 kb on + strand, within Psest_2420 at 2583.449 kb on + strand, within Psest_2420 at 2583.449 kb on + strand, within Psest_2420 at 2583.450 kb on - strand, within Psest_2420 at 2583.450 kb on - strand, within Psest_2420 at 2583.450 kb on - strand, within Psest_2420 at 2583.450 kb on - strand, within Psest_2420 at 2583.450 kb on - strand, within Psest_2420 at 2583.450 kb on - strand, within Psest_2420 at 2583.450 kb on - strand, within Psest_2420 at 2583.709 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.02 mg/ml remove 2,581,408 + Psest_2418 0.69 +0.4 2,581,717 + -0.6 2,581,831 + Psest_2419 0.13 +1.6 2,581,831 + Psest_2419 0.13 +1.8 2,581,832 - Psest_2419 0.13 +3.9 2,581,832 - Psest_2419 0.13 +0.6 2,582,099 - Psest_2419 0.39 -0.9 2,582,099 - Psest_2419 0.39 +0.5 2,582,099 - Psest_2419 0.39 +1.7 2,582,252 - Psest_2419 0.54 +0.2 2,582,321 - Psest_2419 0.60 -0.2 2,582,435 - Psest_2419 0.71 -0.8 2,582,435 - Psest_2419 0.71 +0.8 2,582,435 - Psest_2419 0.71 -0.7 2,582,435 - Psest_2419 0.71 +0.2 2,582,441 - Psest_2419 0.72 -1.2 2,582,590 + Psest_2419 0.86 +1.2 2,582,591 - Psest_2419 0.86 -1.0 2,582,596 + Psest_2419 0.87 +1.6 2,582,596 + Psest_2419 0.87 +0.8 2,582,743 - -1.0 2,582,743 - -0.8 2,582,743 - -0.5 2,582,760 - -0.3 2,583,214 + Psest_2420 0.49 +1.8 2,583,215 - Psest_2420 0.49 +1.7 2,583,215 - Psest_2420 0.49 +1.1 2,583,351 - Psest_2420 0.64 +0.4 2,583,449 + Psest_2420 0.75 -0.2 2,583,449 + Psest_2420 0.75 +0.7 2,583,449 + Psest_2420 0.75 +1.6 2,583,449 + Psest_2420 0.75 -1.1 2,583,450 - Psest_2420 0.76 +0.6 2,583,450 - Psest_2420 0.76 -0.1 2,583,450 - Psest_2420 0.76 +0.1 2,583,450 - Psest_2420 0.76 -1.0 2,583,450 - Psest_2420 0.76 +1.4 2,583,450 - Psest_2420 0.76 -0.9 2,583,450 - Psest_2420 0.76 +0.4 2,583,709 - +0.0
Or see this region's nucleotide sequence