Experiment: Def Carbenicillin 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0020 and Psest_0021 are separated by 74 nucleotides Psest_0021 and Psest_0022 overlap by 4 nucleotides Psest_0022 and Psest_0023 are separated by 55 nucleotides
Psest_0020: Psest_0020 - K+ transport systems, NAD-binding component, at 20,255 to 21,628
_0020
Psest_0021: Psest_0021 - ribosomal RNA small subunit methyltransferase RsmB, at 21,703 to 23,025
_0021
Psest_0022: Psest_0022 - methionyl-tRNA formyltransferase, at 23,022 to 23,966
_0022
Psest_0023: Psest_0023 - peptide deformylase, at 24,022 to 24,528
_0023
Position (kb) 21
22
23
24 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 20.765 kb on + strand, within Psest_0020 at 20.766 kb on - strand, within Psest_0020 at 20.937 kb on + strand, within Psest_0020 at 20.937 kb on + strand, within Psest_0020 at 20.937 kb on + strand, within Psest_0020 at 20.937 kb on + strand, within Psest_0020 at 20.937 kb on + strand, within Psest_0020 at 20.937 kb on + strand, within Psest_0020 at 20.937 kb on + strand, within Psest_0020 at 20.938 kb on - strand, within Psest_0020 at 21.110 kb on + strand, within Psest_0020 at 21.150 kb on + strand, within Psest_0020 at 21.150 kb on + strand, within Psest_0020 at 21.237 kb on + strand, within Psest_0020 at 21.237 kb on + strand, within Psest_0020 at 21.237 kb on + strand, within Psest_0020 at 21.237 kb on + strand, within Psest_0020 at 21.318 kb on + strand, within Psest_0020 at 21.319 kb on - strand, within Psest_0020 at 21.355 kb on + strand, within Psest_0020 at 21.705 kb on - strand at 21.752 kb on + strand at 21.753 kb on - strand at 21.753 kb on - strand at 21.753 kb on - strand at 21.753 kb on - strand at 21.753 kb on - strand at 21.755 kb on + strand at 21.755 kb on + strand at 21.755 kb on + strand at 21.755 kb on + strand at 21.755 kb on + strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.756 kb on - strand at 21.917 kb on + strand, within Psest_0021 at 21.918 kb on - strand, within Psest_0021 at 21.918 kb on - strand, within Psest_0021 at 22.147 kb on + strand, within Psest_0021 at 22.148 kb on - strand, within Psest_0021 at 22.148 kb on - strand, within Psest_0021 at 22.148 kb on - strand, within Psest_0021 at 22.148 kb on - strand, within Psest_0021 at 22.148 kb on - strand, within Psest_0021 at 22.148 kb on - strand, within Psest_0021 at 22.473 kb on - strand, within Psest_0021 at 22.770 kb on - strand, within Psest_0021 at 22.770 kb on - strand, within Psest_0021 at 22.770 kb on - strand, within Psest_0021 at 22.781 kb on + strand, within Psest_0021 at 22.782 kb on - strand, within Psest_0021
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.02 mg/ml remove 20,765 + Psest_0020 0.37 +1.8 20,766 - Psest_0020 0.37 -0.9 20,937 + Psest_0020 0.50 -0.3 20,937 + Psest_0020 0.50 +2.0 20,937 + Psest_0020 0.50 +0.4 20,937 + Psest_0020 0.50 +0.2 20,937 + Psest_0020 0.50 +0.4 20,937 + Psest_0020 0.50 +0.7 20,937 + Psest_0020 0.50 -0.7 20,938 - Psest_0020 0.50 +0.1 21,110 + Psest_0020 0.62 +0.2 21,150 + Psest_0020 0.65 -1.0 21,150 + Psest_0020 0.65 +2.4 21,237 + Psest_0020 0.71 +1.4 21,237 + Psest_0020 0.71 -0.6 21,237 + Psest_0020 0.71 -0.7 21,237 + Psest_0020 0.71 +3.0 21,318 + Psest_0020 0.77 +0.2 21,319 - Psest_0020 0.77 -0.4 21,355 + Psest_0020 0.80 +3.0 21,705 - -1.9 21,752 + -1.3 21,753 - +0.2 21,753 - +2.8 21,753 - +3.1 21,753 - +1.9 21,753 - +1.2 21,755 + -0.4 21,755 + +0.2 21,755 + -1.1 21,755 + -0.1 21,755 + +2.7 21,756 - -2.2 21,756 - -3.0 21,756 - -0.0 21,756 - -0.3 21,756 - -0.8 21,756 - -0.1 21,756 - -0.5 21,756 - -1.2 21,756 - -0.6 21,756 - +0.9 21,756 - +1.6 21,756 - -1.1 21,756 - +1.6 21,756 - +1.2 21,917 + Psest_0021 0.16 +1.4 21,918 - Psest_0021 0.16 -0.1 21,918 - Psest_0021 0.16 -1.3 22,147 + Psest_0021 0.34 -1.0 22,148 - Psest_0021 0.34 -1.6 22,148 - Psest_0021 0.34 +0.4 22,148 - Psest_0021 0.34 +1.9 22,148 - Psest_0021 0.34 +0.4 22,148 - Psest_0021 0.34 -0.7 22,148 - Psest_0021 0.34 +0.4 22,473 - Psest_0021 0.58 +2.8 22,770 - Psest_0021 0.81 +1.3 22,770 - Psest_0021 0.81 +2.8 22,770 - Psest_0021 0.81 -2.3 22,781 + Psest_0021 0.81 +1.4 22,782 - Psest_0021 0.82 +1.9
Or see this region's nucleotide sequence