Experiment: Def Carbenicillin 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2123 and Psest_2124 are separated by 51 nucleotides Psest_2124 and Psest_2125 are separated by 61 nucleotides
Psest_2123: Psest_2123 - fusion of gluconokinase (EC 2.7.1.12) and the small permease component of the D-gluconate TRAP transporter (from data) , at 2,254,074 to 2,255,204
_2123
Psest_2124: Psest_2124 - D-gluconate TRAP transporter, large permease component (from data) , at 2,255,256 to 2,256,533
_2124
Psest_2125: Psest_2125 - D-gluconate TRAP transporter, periplasmic component (from data) , at 2,256,595 to 2,257,611
_2125
Position (kb)
2255
2256
2257 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6
7
8
9
10
11 at 2254.535 kb on - strand, within Psest_2123 at 2254.664 kb on + strand, within Psest_2123 at 2254.665 kb on - strand, within Psest_2123 at 2254.698 kb on + strand, within Psest_2123 at 2255.228 kb on - strand at 2255.228 kb on - strand at 2255.255 kb on + strand at 2255.256 kb on - strand at 2255.256 kb on - strand at 2255.256 kb on - strand at 2255.256 kb on - strand at 2255.256 kb on - strand at 2255.256 kb on - strand at 2255.508 kb on + strand, within Psest_2124 at 2255.509 kb on - strand, within Psest_2124 at 2255.509 kb on - strand, within Psest_2124 at 2255.509 kb on - strand, within Psest_2124 at 2255.730 kb on + strand, within Psest_2124 at 2255.730 kb on + strand, within Psest_2124 at 2255.730 kb on + strand, within Psest_2124 at 2255.731 kb on - strand, within Psest_2124 at 2255.994 kb on + strand, within Psest_2124 at 2255.994 kb on + strand, within Psest_2124 at 2256.123 kb on + strand, within Psest_2124 at 2256.123 kb on + strand, within Psest_2124 at 2256.123 kb on + strand, within Psest_2124 at 2256.123 kb on + strand, within Psest_2124 at 2256.123 kb on + strand, within Psest_2124 at 2256.123 kb on + strand, within Psest_2124 at 2256.124 kb on - strand, within Psest_2124 at 2256.458 kb on + strand at 2256.458 kb on + strand at 2256.459 kb on - strand at 2256.462 kb on + strand at 2256.462 kb on + strand at 2256.462 kb on + strand at 2256.462 kb on + strand at 2256.462 kb on + strand at 2256.462 kb on + strand at 2256.463 kb on - strand at 2256.463 kb on - strand at 2256.463 kb on - strand at 2256.463 kb on - strand at 2256.798 kb on + strand, within Psest_2125 at 2256.798 kb on + strand, within Psest_2125 at 2256.799 kb on - strand, within Psest_2125 at 2256.799 kb on - strand, within Psest_2125 at 2256.799 kb on - strand, within Psest_2125 at 2257.270 kb on + strand, within Psest_2125 at 2257.271 kb on - strand, within Psest_2125 at 2257.285 kb on + strand, within Psest_2125 at 2257.285 kb on + strand, within Psest_2125 at 2257.285 kb on + strand, within Psest_2125 at 2257.309 kb on + strand, within Psest_2125
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.02 mg/ml remove 2,254,535 - Psest_2123 0.41 -0.7 2,254,664 + Psest_2123 0.52 -0.4 2,254,665 - Psest_2123 0.52 -1.5 2,254,698 + Psest_2123 0.55 +1.7 2,255,228 - -2.1 2,255,228 - -3.2 2,255,255 + -1.0 2,255,256 - +0.3 2,255,256 - -1.4 2,255,256 - +1.4 2,255,256 - +10.9 2,255,256 - -0.6 2,255,256 - +0.7 2,255,508 + Psest_2124 0.20 +0.7 2,255,509 - Psest_2124 0.20 +0.4 2,255,509 - Psest_2124 0.20 +1.0 2,255,509 - Psest_2124 0.20 +0.7 2,255,730 + Psest_2124 0.37 -1.8 2,255,730 + Psest_2124 0.37 +0.2 2,255,730 + Psest_2124 0.37 -2.8 2,255,731 - Psest_2124 0.37 +0.1 2,255,994 + Psest_2124 0.58 +1.3 2,255,994 + Psest_2124 0.58 +1.5 2,256,123 + Psest_2124 0.68 +1.2 2,256,123 + Psest_2124 0.68 +2.3 2,256,123 + Psest_2124 0.68 -0.9 2,256,123 + Psest_2124 0.68 +2.3 2,256,123 + Psest_2124 0.68 -1.5 2,256,123 + Psest_2124 0.68 -2.4 2,256,124 - Psest_2124 0.68 +0.4 2,256,458 + +2.2 2,256,458 + -1.5 2,256,459 - +0.6 2,256,462 + +0.1 2,256,462 + -1.4 2,256,462 + -1.3 2,256,462 + -0.4 2,256,462 + -3.5 2,256,462 + +0.7 2,256,463 - +1.6 2,256,463 - -0.2 2,256,463 - +1.5 2,256,463 - -0.0 2,256,798 + Psest_2125 0.20 -2.9 2,256,798 + Psest_2125 0.20 +0.1 2,256,799 - Psest_2125 0.20 -2.1 2,256,799 - Psest_2125 0.20 -2.4 2,256,799 - Psest_2125 0.20 +0.4 2,257,270 + Psest_2125 0.66 +1.6 2,257,271 - Psest_2125 0.66 -0.0 2,257,285 + Psest_2125 0.68 -0.6 2,257,285 + Psest_2125 0.68 -0.7 2,257,285 + Psest_2125 0.68 +0.2 2,257,309 + Psest_2125 0.70 -1.7
Or see this region's nucleotide sequence