Experiment: Def Carbenicillin 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1648 and Psest_1649 are separated by 79 nucleotides Psest_1649 and Psest_1650 are separated by 186 nucleotides Psest_1650 and Psest_1651 are separated by 142 nucleotides
Psest_1648: Psest_1648 - ATP-dependent DNA ligase, at 1,752,859 to 1,754,568
_1648
Psest_1649: Psest_1649 - Predicted exonuclease of the beta-lactamase fold involved in RNA processing, at 1,754,648 to 1,755,661
_1649
Psest_1650: Psest_1650 - hypothetical protein, at 1,755,848 to 1,756,417
_1650
Psest_1651: Psest_1651 - Superfamily II DNA/RNA helicases, SNF2 family, at 1,756,560 to 1,758,890
_1651
Position (kb)
1754
1755
1756 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1754.342 kb on + strand, within Psest_1648 at 1754.342 kb on + strand, within Psest_1648 at 1754.490 kb on - strand at 1754.913 kb on + strand, within Psest_1649 at 1755.015 kb on + strand, within Psest_1649 at 1755.015 kb on + strand, within Psest_1649 at 1755.016 kb on - strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.033 kb on + strand, within Psest_1649 at 1755.034 kb on - strand, within Psest_1649 at 1755.034 kb on - strand, within Psest_1649 at 1755.034 kb on - strand, within Psest_1649 at 1755.034 kb on - strand, within Psest_1649 at 1755.211 kb on + strand, within Psest_1649 at 1755.215 kb on + strand, within Psest_1649 at 1755.216 kb on - strand, within Psest_1649 at 1755.216 kb on - strand, within Psest_1649 at 1755.216 kb on - strand, within Psest_1649 at 1755.240 kb on + strand, within Psest_1649 at 1755.240 kb on + strand, within Psest_1649 at 1755.240 kb on + strand, within Psest_1649 at 1755.241 kb on - strand, within Psest_1649 at 1755.330 kb on + strand, within Psest_1649 at 1755.330 kb on + strand, within Psest_1649 at 1755.331 kb on - strand, within Psest_1649 at 1755.363 kb on + strand, within Psest_1649 at 1755.363 kb on + strand, within Psest_1649 at 1755.364 kb on - strand, within Psest_1649 at 1755.450 kb on + strand, within Psest_1649 at 1755.526 kb on - strand, within Psest_1649 at 1755.606 kb on + strand at 1755.606 kb on + strand at 1755.627 kb on + strand at 1755.627 kb on + strand at 1755.665 kb on + strand at 1755.666 kb on - strand at 1755.686 kb on + strand at 1755.687 kb on - strand at 1755.687 kb on - strand at 1755.687 kb on - strand at 1755.785 kb on + strand at 1755.785 kb on + strand at 1755.787 kb on + strand at 1755.787 kb on + strand at 1755.787 kb on + strand at 1755.787 kb on + strand at 1755.787 kb on + strand at 1755.787 kb on + strand at 1755.787 kb on + strand at 1755.788 kb on - strand at 1755.788 kb on - strand at 1755.788 kb on - strand at 1755.788 kb on - strand at 1755.788 kb on - strand at 1755.788 kb on - strand at 1755.788 kb on - strand at 1756.056 kb on + strand, within Psest_1650 at 1756.056 kb on + strand, within Psest_1650 at 1756.056 kb on + strand, within Psest_1650 at 1756.056 kb on + strand, within Psest_1650 at 1756.056 kb on + strand, within Psest_1650 at 1756.056 kb on + strand, within Psest_1650 at 1756.056 kb on + strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.057 kb on - strand, within Psest_1650 at 1756.208 kb on + strand, within Psest_1650 at 1756.208 kb on + strand, within Psest_1650
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.02 mg/ml remove 1,754,342 + Psest_1648 0.87 +1.5 1,754,342 + Psest_1648 0.87 -2.8 1,754,490 - +1.4 1,754,913 + Psest_1649 0.26 -0.5 1,755,015 + Psest_1649 0.36 -0.1 1,755,015 + Psest_1649 0.36 -2.9 1,755,016 - Psest_1649 0.36 +0.8 1,755,033 + Psest_1649 0.38 -1.5 1,755,033 + Psest_1649 0.38 +0.9 1,755,033 + Psest_1649 0.38 -1.4 1,755,033 + Psest_1649 0.38 +1.7 1,755,033 + Psest_1649 0.38 -2.0 1,755,033 + Psest_1649 0.38 +0.9 1,755,033 + Psest_1649 0.38 +0.6 1,755,033 + Psest_1649 0.38 +4.5 1,755,033 + Psest_1649 0.38 -0.9 1,755,034 - Psest_1649 0.38 +0.8 1,755,034 - Psest_1649 0.38 +0.2 1,755,034 - Psest_1649 0.38 -3.0 1,755,034 - Psest_1649 0.38 -0.3 1,755,211 + Psest_1649 0.56 -2.6 1,755,215 + Psest_1649 0.56 -1.3 1,755,216 - Psest_1649 0.56 -1.0 1,755,216 - Psest_1649 0.56 +1.0 1,755,216 - Psest_1649 0.56 -1.3 1,755,240 + Psest_1649 0.58 -0.9 1,755,240 + Psest_1649 0.58 -0.3 1,755,240 + Psest_1649 0.58 -2.9 1,755,241 - Psest_1649 0.58 +1.6 1,755,330 + Psest_1649 0.67 -1.8 1,755,330 + Psest_1649 0.67 -2.6 1,755,331 - Psest_1649 0.67 -2.7 1,755,363 + Psest_1649 0.71 -2.6 1,755,363 + Psest_1649 0.71 +0.4 1,755,364 - Psest_1649 0.71 -0.3 1,755,450 + Psest_1649 0.79 -1.7 1,755,526 - Psest_1649 0.87 +1.9 1,755,606 + -2.4 1,755,606 + +0.3 1,755,627 + -2.0 1,755,627 + -1.0 1,755,665 + -1.7 1,755,666 - -0.1 1,755,686 + -0.8 1,755,687 - -2.5 1,755,687 - -0.1 1,755,687 - -0.3 1,755,785 + +1.3 1,755,785 + +0.9 1,755,787 + -0.9 1,755,787 + -1.2 1,755,787 + -2.5 1,755,787 + +0.5 1,755,787 + +0.2 1,755,787 + -0.3 1,755,787 + +0.6 1,755,788 - +2.1 1,755,788 - +3.2 1,755,788 - -2.6 1,755,788 - -3.7 1,755,788 - +0.5 1,755,788 - -0.4 1,755,788 - -0.5 1,756,056 + Psest_1650 0.36 -0.9 1,756,056 + Psest_1650 0.36 +1.7 1,756,056 + Psest_1650 0.36 -2.3 1,756,056 + Psest_1650 0.36 -1.6 1,756,056 + Psest_1650 0.36 +3.5 1,756,056 + Psest_1650 0.36 -1.0 1,756,056 + Psest_1650 0.36 +0.1 1,756,057 - Psest_1650 0.37 -2.1 1,756,057 - Psest_1650 0.37 -0.4 1,756,057 - Psest_1650 0.37 -0.2 1,756,057 - Psest_1650 0.37 +1.0 1,756,057 - Psest_1650 0.37 +0.1 1,756,057 - Psest_1650 0.37 -0.9 1,756,057 - Psest_1650 0.37 +0.1 1,756,057 - Psest_1650 0.37 -0.5 1,756,057 - Psest_1650 0.37 -1.3 1,756,057 - Psest_1650 0.37 -0.8 1,756,208 + Psest_1650 0.63 -1.3 1,756,208 + Psest_1650 0.63 -0.1
Or see this region's nucleotide sequence