Experiment: Def Carbenicillin 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1630 and Psest_1631 are separated by 96 nucleotides Psest_1631 and Psest_1632 are separated by 0 nucleotides Psest_1632 and Psest_1633 are separated by 179 nucleotides Psest_1633 and Psest_1634 are separated by 103 nucleotides
Psest_1630: Psest_1630 - Transcriptional regulator, at 1,731,417 to 1,732,271
_1630
Psest_1631: Psest_1631 - Uncharacterized conserved small protein, at 1,732,368 to 1,732,604
_1631
Psest_1632: Psest_1632 - Predicted permeases, at 1,732,605 to 1,733,360
_1632
Psest_1633: Psest_1633 - Protein of unknown function (DUF3365)., at 1,733,540 to 1,734,097
_1633
Psest_1634: Psest_1634 - tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain, at 1,734,201 to 1,736,183
_1634
Position (kb)
1732
1733
1734 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1731.639 kb on - strand, within Psest_1630 at 1731.640 kb on + strand, within Psest_1630 at 1731.641 kb on - strand, within Psest_1630 at 1731.796 kb on - strand, within Psest_1630 at 1731.796 kb on - strand, within Psest_1630 at 1731.796 kb on - strand, within Psest_1630 at 1731.861 kb on + strand, within Psest_1630 at 1731.861 kb on + strand, within Psest_1630 at 1731.861 kb on + strand, within Psest_1630 at 1731.861 kb on + strand, within Psest_1630 at 1731.862 kb on - strand, within Psest_1630 at 1731.862 kb on - strand, within Psest_1630 at 1731.862 kb on - strand, within Psest_1630 at 1731.955 kb on + strand, within Psest_1630 at 1731.955 kb on + strand, within Psest_1630 at 1732.063 kb on - strand, within Psest_1630 at 1732.063 kb on - strand, within Psest_1630 at 1732.262 kb on - strand at 1732.322 kb on + strand at 1732.373 kb on + strand at 1732.374 kb on - strand at 1732.394 kb on + strand, within Psest_1631 at 1732.394 kb on + strand, within Psest_1631 at 1732.395 kb on - strand, within Psest_1631 at 1732.699 kb on + strand, within Psest_1632 at 1732.700 kb on - strand, within Psest_1632 at 1732.700 kb on - strand, within Psest_1632 at 1732.700 kb on - strand, within Psest_1632 at 1732.792 kb on + strand, within Psest_1632 at 1732.793 kb on - strand, within Psest_1632 at 1732.793 kb on - strand, within Psest_1632 at 1733.352 kb on + strand at 1733.352 kb on + strand at 1733.352 kb on + strand at 1733.353 kb on - strand at 1733.353 kb on - strand at 1733.353 kb on - strand at 1733.353 kb on - strand at 1733.353 kb on - strand at 1733.353 kb on - strand at 1733.353 kb on - strand at 1733.353 kb on - strand at 1733.461 kb on + strand at 1733.461 kb on + strand at 1733.461 kb on + strand at 1733.462 kb on - strand at 1733.462 kb on - strand at 1733.462 kb on - strand at 1733.511 kb on + strand at 1733.639 kb on + strand, within Psest_1633 at 1733.639 kb on + strand, within Psest_1633 at 1733.639 kb on + strand, within Psest_1633 at 1733.639 kb on + strand, within Psest_1633 at 1733.640 kb on - strand, within Psest_1633 at 1733.642 kb on + strand, within Psest_1633 at 1733.642 kb on + strand, within Psest_1633 at 1733.642 kb on + strand, within Psest_1633 at 1733.642 kb on + strand, within Psest_1633 at 1733.642 kb on + strand, within Psest_1633 at 1733.642 kb on + strand, within Psest_1633 at 1733.642 kb on + strand, within Psest_1633 at 1733.643 kb on - strand, within Psest_1633 at 1733.643 kb on - strand, within Psest_1633 at 1733.643 kb on - strand, within Psest_1633 at 1733.709 kb on + strand, within Psest_1633 at 1733.710 kb on - strand, within Psest_1633 at 1734.056 kb on + strand at 1734.109 kb on + strand at 1734.110 kb on - strand at 1734.206 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.02 mg/ml remove 1,731,639 - Psest_1630 0.26 -1.7 1,731,640 + Psest_1630 0.26 +0.2 1,731,641 - Psest_1630 0.26 -0.0 1,731,796 - Psest_1630 0.44 +1.7 1,731,796 - Psest_1630 0.44 -0.3 1,731,796 - Psest_1630 0.44 -0.7 1,731,861 + Psest_1630 0.52 -0.6 1,731,861 + Psest_1630 0.52 -2.0 1,731,861 + Psest_1630 0.52 +0.4 1,731,861 + Psest_1630 0.52 +0.4 1,731,862 - Psest_1630 0.52 +1.2 1,731,862 - Psest_1630 0.52 -1.3 1,731,862 - Psest_1630 0.52 -1.0 1,731,955 + Psest_1630 0.63 +1.5 1,731,955 + Psest_1630 0.63 +0.9 1,732,063 - Psest_1630 0.76 -1.0 1,732,063 - Psest_1630 0.76 +0.0 1,732,262 - +1.7 1,732,322 + +0.6 1,732,373 + -0.7 1,732,374 - -1.2 1,732,394 + Psest_1631 0.11 +2.2 1,732,394 + Psest_1631 0.11 -1.0 1,732,395 - Psest_1631 0.11 +1.4 1,732,699 + Psest_1632 0.12 -0.1 1,732,700 - Psest_1632 0.13 +3.2 1,732,700 - Psest_1632 0.13 +1.3 1,732,700 - Psest_1632 0.13 +1.9 1,732,792 + Psest_1632 0.25 +0.4 1,732,793 - Psest_1632 0.25 +0.9 1,732,793 - Psest_1632 0.25 +1.0 1,733,352 + +2.1 1,733,352 + -1.5 1,733,352 + -0.1 1,733,353 - +0.7 1,733,353 - -0.9 1,733,353 - -0.1 1,733,353 - +0.8 1,733,353 - +0.9 1,733,353 - +0.6 1,733,353 - -1.6 1,733,353 - +2.1 1,733,461 + +1.7 1,733,461 + -2.4 1,733,461 + +0.6 1,733,462 - +2.1 1,733,462 - +0.8 1,733,462 - +0.5 1,733,511 + +1.6 1,733,639 + Psest_1633 0.18 -1.2 1,733,639 + Psest_1633 0.18 +0.2 1,733,639 + Psest_1633 0.18 +2.6 1,733,639 + Psest_1633 0.18 -0.8 1,733,640 - Psest_1633 0.18 +0.6 1,733,642 + Psest_1633 0.18 -0.3 1,733,642 + Psest_1633 0.18 -0.4 1,733,642 + Psest_1633 0.18 +1.2 1,733,642 + Psest_1633 0.18 -2.5 1,733,642 + Psest_1633 0.18 +0.3 1,733,642 + Psest_1633 0.18 -0.6 1,733,642 + Psest_1633 0.18 +0.4 1,733,643 - Psest_1633 0.18 -0.5 1,733,643 - Psest_1633 0.18 +0.9 1,733,643 - Psest_1633 0.18 +0.7 1,733,709 + Psest_1633 0.30 -1.5 1,733,710 - Psest_1633 0.30 +0.6 1,734,056 + +0.5 1,734,109 + -0.4 1,734,110 - -0.5 1,734,206 + -1.1
Or see this region's nucleotide sequence