Experiment: Def Carbenicillin 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1484 and Psest_1485 are separated by 10 nucleotides Psest_1485 and Psest_1486 are separated by 5 nucleotides Psest_1486 and Psest_1487 are separated by 9 nucleotides
Psest_1484: Psest_1484 - Thiol-disulfide isomerase and thioredoxins, at 1,586,799 to 1,587,257
_1484
Psest_1485: Psest_1485 - Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains), at 1,587,268 to 1,587,840
_1485
Psest_1486: Psest_1486 - hypothetical protein, at 1,587,846 to 1,588,778
_1486
Psest_1487: Psest_1487 - prolyl-tRNA synthetase, family II, at 1,588,788 to 1,590,503
_1487
Position (kb)
1587
1588
1589 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1586.918 kb on + strand, within Psest_1484 at 1586.918 kb on + strand, within Psest_1484 at 1586.919 kb on - strand, within Psest_1484 at 1586.919 kb on - strand, within Psest_1484 at 1586.919 kb on - strand, within Psest_1484 at 1586.937 kb on + strand, within Psest_1484 at 1586.938 kb on - strand, within Psest_1484 at 1586.938 kb on - strand, within Psest_1484 at 1586.938 kb on - strand, within Psest_1484 at 1586.967 kb on - strand, within Psest_1484 at 1586.984 kb on + strand, within Psest_1484 at 1586.984 kb on + strand, within Psest_1484 at 1586.984 kb on + strand, within Psest_1484 at 1586.984 kb on + strand, within Psest_1484 at 1586.984 kb on + strand, within Psest_1484 at 1586.984 kb on + strand, within Psest_1484 at 1586.985 kb on - strand, within Psest_1484 at 1586.987 kb on + strand, within Psest_1484 at 1586.987 kb on + strand, within Psest_1484 at 1586.987 kb on + strand, within Psest_1484 at 1586.996 kb on + strand, within Psest_1484 at 1587.125 kb on + strand, within Psest_1484 at 1587.159 kb on + strand, within Psest_1484 at 1587.159 kb on + strand, within Psest_1484 at 1587.159 kb on + strand, within Psest_1484 at 1587.159 kb on + strand, within Psest_1484 at 1587.159 kb on + strand, within Psest_1484 at 1587.159 kb on + strand, within Psest_1484 at 1587.159 kb on + strand, within Psest_1484 at 1587.159 kb on + strand, within Psest_1484 at 1587.160 kb on - strand, within Psest_1484 at 1587.160 kb on - strand, within Psest_1484 at 1587.160 kb on - strand, within Psest_1484 at 1587.160 kb on - strand, within Psest_1484 at 1587.160 kb on - strand, within Psest_1484 at 1587.160 kb on - strand, within Psest_1484 at 1587.160 kb on - strand, within Psest_1484 at 1587.160 kb on - strand, within Psest_1484 at 1587.160 kb on - strand, within Psest_1484 at 1587.224 kb on - strand at 1587.255 kb on + strand at 1587.281 kb on + strand at 1587.392 kb on + strand, within Psest_1485 at 1587.392 kb on + strand, within Psest_1485 at 1587.393 kb on - strand, within Psest_1485 at 1587.393 kb on - strand, within Psest_1485 at 1587.395 kb on + strand, within Psest_1485 at 1587.395 kb on + strand, within Psest_1485 at 1587.395 kb on + strand, within Psest_1485 at 1587.395 kb on + strand, within Psest_1485 at 1587.395 kb on + strand, within Psest_1485 at 1587.395 kb on + strand, within Psest_1485 at 1587.395 kb on + strand, within Psest_1485 at 1587.463 kb on + strand, within Psest_1485 at 1587.651 kb on - strand, within Psest_1485 at 1587.677 kb on - strand, within Psest_1485 at 1587.677 kb on - strand, within Psest_1485 at 1588.280 kb on - strand, within Psest_1486 at 1588.280 kb on - strand, within Psest_1486 at 1588.456 kb on - strand, within Psest_1486 at 1588.615 kb on - strand, within Psest_1486 at 1588.647 kb on - strand, within Psest_1486
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.02 mg/ml remove 1,586,918 + Psest_1484 0.26 -0.5 1,586,918 + Psest_1484 0.26 -1.5 1,586,919 - Psest_1484 0.26 +0.1 1,586,919 - Psest_1484 0.26 -3.5 1,586,919 - Psest_1484 0.26 -0.6 1,586,937 + Psest_1484 0.30 +0.2 1,586,938 - Psest_1484 0.30 +0.1 1,586,938 - Psest_1484 0.30 -0.0 1,586,938 - Psest_1484 0.30 -0.6 1,586,967 - Psest_1484 0.37 +0.2 1,586,984 + Psest_1484 0.40 -1.2 1,586,984 + Psest_1484 0.40 -0.4 1,586,984 + Psest_1484 0.40 -1.0 1,586,984 + Psest_1484 0.40 -1.2 1,586,984 + Psest_1484 0.40 -0.1 1,586,984 + Psest_1484 0.40 -1.5 1,586,985 - Psest_1484 0.41 +2.8 1,586,987 + Psest_1484 0.41 -2.4 1,586,987 + Psest_1484 0.41 -1.6 1,586,987 + Psest_1484 0.41 +0.5 1,586,996 + Psest_1484 0.43 -1.8 1,587,125 + Psest_1484 0.71 -0.1 1,587,159 + Psest_1484 0.78 -0.1 1,587,159 + Psest_1484 0.78 +1.6 1,587,159 + Psest_1484 0.78 -0.7 1,587,159 + Psest_1484 0.78 -0.7 1,587,159 + Psest_1484 0.78 -2.1 1,587,159 + Psest_1484 0.78 -1.7 1,587,159 + Psest_1484 0.78 -1.1 1,587,159 + Psest_1484 0.78 +2.2 1,587,160 - Psest_1484 0.79 +0.0 1,587,160 - Psest_1484 0.79 -0.2 1,587,160 - Psest_1484 0.79 -0.9 1,587,160 - Psest_1484 0.79 -0.5 1,587,160 - Psest_1484 0.79 -1.1 1,587,160 - Psest_1484 0.79 +0.6 1,587,160 - Psest_1484 0.79 +0.9 1,587,160 - Psest_1484 0.79 -0.4 1,587,160 - Psest_1484 0.79 -0.6 1,587,224 - -0.2 1,587,255 + +0.1 1,587,281 + -1.1 1,587,392 + Psest_1485 0.22 -1.2 1,587,392 + Psest_1485 0.22 -0.0 1,587,393 - Psest_1485 0.22 -3.1 1,587,393 - Psest_1485 0.22 -3.2 1,587,395 + Psest_1485 0.22 -0.8 1,587,395 + Psest_1485 0.22 -0.9 1,587,395 + Psest_1485 0.22 +0.7 1,587,395 + Psest_1485 0.22 -1.2 1,587,395 + Psest_1485 0.22 -0.5 1,587,395 + Psest_1485 0.22 +0.1 1,587,395 + Psest_1485 0.22 -1.5 1,587,463 + Psest_1485 0.34 -2.8 1,587,651 - Psest_1485 0.67 -2.9 1,587,677 - Psest_1485 0.71 -2.1 1,587,677 - Psest_1485 0.71 +0.9 1,588,280 - Psest_1486 0.47 -0.8 1,588,280 - Psest_1486 0.47 -0.8 1,588,456 - Psest_1486 0.65 -1.0 1,588,615 - Psest_1486 0.82 +0.6 1,588,647 - Psest_1486 0.86 -1.7
Or see this region's nucleotide sequence