Experiment: Def Carbenicillin 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1464 and Psest_1465 are separated by 75 nucleotides Psest_1465 and Psest_1466 are separated by 43 nucleotides Psest_1466 and Psest_1467 are separated by 130 nucleotides
Psest_1464: Psest_1464 - Selenocysteine lyase, at 1,571,595 to 1,572,800
_1464
Psest_1465: Psest_1465 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 1,572,876 to 1,573,910
_1465
Psest_1466: Psest_1466 - transcriptional regulator, Spx/MgsR family, at 1,573,954 to 1,574,325
_1466
Psest_1467: Psest_1467 - Thioredoxin., at 1,574,456 to 1,574,881
_1467
Position (kb)
1572
1573
1574 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1571.935 kb on + strand, within Psest_1464 at 1572.028 kb on + strand, within Psest_1464 at 1572.029 kb on - strand, within Psest_1464 at 1572.081 kb on + strand, within Psest_1464 at 1572.100 kb on + strand, within Psest_1464 at 1572.101 kb on - strand, within Psest_1464 at 1572.151 kb on + strand, within Psest_1464 at 1572.151 kb on + strand, within Psest_1464 at 1572.151 kb on + strand, within Psest_1464 at 1572.151 kb on + strand, within Psest_1464 at 1572.152 kb on - strand, within Psest_1464 at 1572.152 kb on - strand, within Psest_1464 at 1572.152 kb on - strand, within Psest_1464 at 1572.152 kb on - strand, within Psest_1464 at 1572.152 kb on - strand, within Psest_1464 at 1572.152 kb on - strand, within Psest_1464 at 1572.253 kb on - strand, within Psest_1464 at 1572.253 kb on - strand, within Psest_1464 at 1572.253 kb on - strand, within Psest_1464 at 1572.667 kb on + strand, within Psest_1464 at 1572.667 kb on + strand, within Psest_1464 at 1572.667 kb on + strand, within Psest_1464 at 1572.667 kb on + strand, within Psest_1464 at 1572.667 kb on + strand, within Psest_1464 at 1572.668 kb on - strand, within Psest_1464 at 1572.668 kb on - strand, within Psest_1464 at 1572.670 kb on + strand, within Psest_1464 at 1572.730 kb on + strand at 1572.730 kb on + strand at 1572.882 kb on - strand at 1572.882 kb on - strand at 1573.983 kb on - strand at 1573.983 kb on - strand at 1574.014 kb on - strand, within Psest_1466 at 1574.030 kb on - strand, within Psest_1466 at 1574.198 kb on - strand, within Psest_1466 at 1574.214 kb on - strand, within Psest_1466 at 1574.214 kb on - strand, within Psest_1466 at 1574.257 kb on - strand, within Psest_1466 at 1574.326 kb on - strand at 1574.326 kb on - strand at 1574.368 kb on - strand at 1574.766 kb on + strand, within Psest_1467 at 1574.766 kb on + strand, within Psest_1467 at 1574.766 kb on + strand, within Psest_1467 at 1574.766 kb on + strand, within Psest_1467 at 1574.766 kb on + strand, within Psest_1467 at 1574.767 kb on - strand, within Psest_1467 at 1574.767 kb on - strand, within Psest_1467 at 1574.767 kb on - strand, within Psest_1467 at 1574.777 kb on + strand, within Psest_1467 at 1574.777 kb on + strand, within Psest_1467 at 1574.777 kb on + strand, within Psest_1467 at 1574.777 kb on + strand, within Psest_1467 at 1574.777 kb on + strand, within Psest_1467 at 1574.777 kb on + strand, within Psest_1467 at 1574.777 kb on + strand, within Psest_1467 at 1574.777 kb on + strand, within Psest_1467 at 1574.777 kb on + strand, within Psest_1467 at 1574.778 kb on - strand, within Psest_1467 at 1574.778 kb on - strand, within Psest_1467 at 1574.778 kb on - strand, within Psest_1467 at 1574.802 kb on + strand, within Psest_1467 at 1574.803 kb on - strand, within Psest_1467 at 1574.803 kb on - strand, within Psest_1467 at 1574.861 kb on + strand at 1574.861 kb on + strand at 1574.861 kb on + strand at 1574.862 kb on - strand at 1574.862 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.02 mg/ml remove 1,571,935 + Psest_1464 0.28 -0.8 1,572,028 + Psest_1464 0.36 -0.1 1,572,029 - Psest_1464 0.36 -2.0 1,572,081 + Psest_1464 0.40 -0.5 1,572,100 + Psest_1464 0.42 -0.1 1,572,101 - Psest_1464 0.42 -1.1 1,572,151 + Psest_1464 0.46 -0.8 1,572,151 + Psest_1464 0.46 -1.5 1,572,151 + Psest_1464 0.46 -0.7 1,572,151 + Psest_1464 0.46 +1.4 1,572,152 - Psest_1464 0.46 -0.2 1,572,152 - Psest_1464 0.46 -0.8 1,572,152 - Psest_1464 0.46 -2.0 1,572,152 - Psest_1464 0.46 -1.0 1,572,152 - Psest_1464 0.46 -1.8 1,572,152 - Psest_1464 0.46 -1.2 1,572,253 - Psest_1464 0.55 +0.3 1,572,253 - Psest_1464 0.55 -2.2 1,572,253 - Psest_1464 0.55 -0.5 1,572,667 + Psest_1464 0.89 +1.7 1,572,667 + Psest_1464 0.89 -4.3 1,572,667 + Psest_1464 0.89 +1.4 1,572,667 + Psest_1464 0.89 -0.5 1,572,667 + Psest_1464 0.89 +0.2 1,572,668 - Psest_1464 0.89 -2.6 1,572,668 - Psest_1464 0.89 -1.4 1,572,670 + Psest_1464 0.89 +0.1 1,572,730 + -0.5 1,572,730 + +0.7 1,572,882 - +0.7 1,572,882 - +1.0 1,573,983 - +0.2 1,573,983 - -0.9 1,574,014 - Psest_1466 0.16 +0.6 1,574,030 - Psest_1466 0.20 -0.8 1,574,198 - Psest_1466 0.66 -0.2 1,574,214 - Psest_1466 0.70 -0.4 1,574,214 - Psest_1466 0.70 +0.4 1,574,257 - Psest_1466 0.81 -0.1 1,574,326 - +0.1 1,574,326 - +0.6 1,574,368 - -0.8 1,574,766 + Psest_1467 0.73 -2.3 1,574,766 + Psest_1467 0.73 +0.4 1,574,766 + Psest_1467 0.73 +0.5 1,574,766 + Psest_1467 0.73 -2.6 1,574,766 + Psest_1467 0.73 -0.9 1,574,767 - Psest_1467 0.73 -2.6 1,574,767 - Psest_1467 0.73 -1.4 1,574,767 - Psest_1467 0.73 -2.4 1,574,777 + Psest_1467 0.75 +4.4 1,574,777 + Psest_1467 0.75 -0.3 1,574,777 + Psest_1467 0.75 -2.6 1,574,777 + Psest_1467 0.75 -0.1 1,574,777 + Psest_1467 0.75 -0.1 1,574,777 + Psest_1467 0.75 -1.7 1,574,777 + Psest_1467 0.75 +0.7 1,574,777 + Psest_1467 0.75 +0.1 1,574,777 + Psest_1467 0.75 +0.2 1,574,778 - Psest_1467 0.76 -0.0 1,574,778 - Psest_1467 0.76 +1.3 1,574,778 - Psest_1467 0.76 -0.8 1,574,802 + Psest_1467 0.81 -0.8 1,574,803 - Psest_1467 0.81 -2.6 1,574,803 - Psest_1467 0.81 -1.4 1,574,861 + -0.5 1,574,861 + +0.3 1,574,861 + -0.9 1,574,862 - -1.3 1,574,862 - +0.9
Or see this region's nucleotide sequence