Experiment: Def Carbenicillin 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1307 and Psest_1308 are separated by 164 nucleotides Psest_1308 and Psest_1309 are separated by 2 nucleotides Psest_1309 and Psest_1310 overlap by 4 nucleotides
Psest_1307: Psest_1307 - Dienelactone hydrolase and related enzymes, at 1,408,189 to 1,408,995
_1307
Psest_1308: Psest_1308 - ABC-type branched-chain amino acid transport systems, ATPase component, at 1,409,160 to 1,409,861
_1308
Psest_1309: Psest_1309 - ABC-type branched-chain amino acid transport systems, ATPase component, at 1,409,864 to 1,410,631
_1309
Psest_1310: Psest_1310 - ABC-type branched-chain amino acid transport system, permease component, at 1,410,628 to 1,411,884
_1310
Position (kb)
1409
1410 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1408.368 kb on - strand, within Psest_1307 at 1408.584 kb on - strand, within Psest_1307 at 1408.584 kb on - strand, within Psest_1307 at 1408.742 kb on + strand, within Psest_1307 at 1408.743 kb on - strand, within Psest_1307 at 1408.880 kb on + strand, within Psest_1307 at 1408.880 kb on + strand, within Psest_1307 at 1408.881 kb on - strand, within Psest_1307 at 1408.881 kb on - strand, within Psest_1307 at 1408.884 kb on - strand, within Psest_1307 at 1408.917 kb on - strand at 1409.045 kb on + strand at 1409.173 kb on + strand at 1409.174 kb on - strand at 1409.174 kb on - strand at 1409.247 kb on + strand, within Psest_1308 at 1409.247 kb on + strand, within Psest_1308 at 1409.248 kb on - strand, within Psest_1308 at 1409.248 kb on - strand, within Psest_1308 at 1409.248 kb on - strand, within Psest_1308 at 1409.248 kb on - strand, within Psest_1308 at 1409.248 kb on - strand, within Psest_1308 at 1409.248 kb on - strand, within Psest_1308 at 1409.248 kb on - strand, within Psest_1308 at 1409.248 kb on - strand, within Psest_1308 at 1409.252 kb on - strand, within Psest_1308 at 1409.252 kb on - strand, within Psest_1308 at 1409.377 kb on - strand, within Psest_1308 at 1409.830 kb on + strand at 1409.865 kb on + strand at 1409.865 kb on + strand at 1409.865 kb on + strand at 1409.865 kb on + strand at 1409.865 kb on + strand at 1409.866 kb on - strand at 1409.866 kb on - strand at 1409.958 kb on + strand, within Psest_1309 at 1409.958 kb on + strand, within Psest_1309 at 1409.958 kb on + strand, within Psest_1309 at 1409.958 kb on + strand, within Psest_1309 at 1409.958 kb on + strand, within Psest_1309 at 1409.958 kb on + strand, within Psest_1309 at 1409.958 kb on + strand, within Psest_1309 at 1409.958 kb on + strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1409.959 kb on - strand, within Psest_1309 at 1410.166 kb on - strand, within Psest_1309 at 1410.302 kb on - strand, within Psest_1309 at 1410.383 kb on + strand, within Psest_1309 at 1410.384 kb on - strand, within Psest_1309 at 1410.469 kb on - strand, within Psest_1309 at 1410.548 kb on + strand, within Psest_1309 at 1410.549 kb on - strand, within Psest_1309 at 1410.608 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.02 mg/ml remove 1,408,368 - Psest_1307 0.22 -2.1 1,408,584 - Psest_1307 0.49 +0.4 1,408,584 - Psest_1307 0.49 -0.7 1,408,742 + Psest_1307 0.69 -2.1 1,408,743 - Psest_1307 0.69 +0.2 1,408,880 + Psest_1307 0.86 +1.3 1,408,880 + Psest_1307 0.86 -1.1 1,408,881 - Psest_1307 0.86 +0.2 1,408,881 - Psest_1307 0.86 -3.3 1,408,884 - Psest_1307 0.86 +0.4 1,408,917 - +1.4 1,409,045 + +0.5 1,409,173 + -0.9 1,409,174 - -0.6 1,409,174 - -0.2 1,409,247 + Psest_1308 0.12 +0.1 1,409,247 + Psest_1308 0.12 -0.0 1,409,248 - Psest_1308 0.13 -0.7 1,409,248 - Psest_1308 0.13 -3.1 1,409,248 - Psest_1308 0.13 +0.8 1,409,248 - Psest_1308 0.13 -1.1 1,409,248 - Psest_1308 0.13 -1.6 1,409,248 - Psest_1308 0.13 +0.7 1,409,248 - Psest_1308 0.13 -2.0 1,409,248 - Psest_1308 0.13 -2.1 1,409,252 - Psest_1308 0.13 -3.0 1,409,252 - Psest_1308 0.13 +0.4 1,409,377 - Psest_1308 0.31 -0.2 1,409,830 + +0.5 1,409,865 + -1.5 1,409,865 + -0.9 1,409,865 + +0.4 1,409,865 + +0.0 1,409,865 + +0.1 1,409,866 - +0.2 1,409,866 - -0.1 1,409,958 + Psest_1309 0.12 -1.9 1,409,958 + Psest_1309 0.12 -2.1 1,409,958 + Psest_1309 0.12 -2.5 1,409,958 + Psest_1309 0.12 -1.4 1,409,958 + Psest_1309 0.12 -2.0 1,409,958 + Psest_1309 0.12 -0.5 1,409,958 + Psest_1309 0.12 +0.2 1,409,958 + Psest_1309 0.12 +2.2 1,409,959 - Psest_1309 0.12 +0.7 1,409,959 - Psest_1309 0.12 +1.3 1,409,959 - Psest_1309 0.12 -3.1 1,409,959 - Psest_1309 0.12 +0.6 1,409,959 - Psest_1309 0.12 -3.5 1,409,959 - Psest_1309 0.12 -3.3 1,409,959 - Psest_1309 0.12 +1.0 1,409,959 - Psest_1309 0.12 -0.4 1,409,959 - Psest_1309 0.12 +2.4 1,409,959 - Psest_1309 0.12 -3.0 1,409,959 - Psest_1309 0.12 -0.6 1,409,959 - Psest_1309 0.12 +0.3 1,409,959 - Psest_1309 0.12 -1.1 1,410,166 - Psest_1309 0.39 -2.5 1,410,302 - Psest_1309 0.57 +0.1 1,410,383 + Psest_1309 0.68 -2.0 1,410,384 - Psest_1309 0.68 -1.4 1,410,469 - Psest_1309 0.79 +2.0 1,410,548 + Psest_1309 0.89 +0.2 1,410,549 - Psest_1309 0.89 -2.0 1,410,608 + -2.0
Or see this region's nucleotide sequence