Experiment: Def Carbenicillin 0.02 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0114 and Psest_0115 are separated by 191 nucleotides Psest_0115 and Psest_0116 are separated by 16 nucleotides Psest_0116 and Psest_0117 overlap by 4 nucleotides Psest_0117 and Psest_0118 overlap by 4 nucleotides
Psest_0114: Psest_0114 - RNA polymerase sigma factor, sigma-70 family, at 112,411 to 112,980
_0114
Psest_0115: Psest_0115 - Predicted integral membrane protein, at 113,172 to 113,444
_0115
Psest_0116: Psest_0116 - Uncharacterized protein conserved in bacteria, at 113,461 to 114,288
_0116
Psest_0117: Psest_0117 - Uncharacterized protein conserved in bacteria (DUF2063)., at 114,285 to 115,076
_0117
Psest_0118: Psest_0118 - Predicted membrane protein, at 115,073 to 115,534
_0118
Position (kb)
113
114
115 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 112.577 kb on - strand, within Psest_0114 at 112.789 kb on + strand, within Psest_0114 at 112.789 kb on + strand, within Psest_0114 at 112.968 kb on + strand at 112.969 kb on - strand at 112.969 kb on - strand at 113.112 kb on + strand at 113.112 kb on + strand at 113.112 kb on + strand at 113.112 kb on + strand at 113.113 kb on - strand at 113.113 kb on - strand at 113.113 kb on - strand at 113.113 kb on - strand at 113.113 kb on - strand at 113.384 kb on + strand, within Psest_0115 at 113.448 kb on + strand at 113.449 kb on - strand at 113.484 kb on - strand at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.563 kb on + strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.564 kb on - strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.767 kb on + strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.768 kb on - strand, within Psest_0116 at 113.888 kb on - strand, within Psest_0116 at 113.888 kb on - strand, within Psest_0116 at 114.505 kb on - strand, within Psest_0117 at 114.505 kb on - strand, within Psest_0117 at 114.505 kb on - strand, within Psest_0117 at 114.547 kb on - strand, within Psest_0117 at 114.603 kb on + strand, within Psest_0117 at 114.604 kb on - strand, within Psest_0117 at 114.604 kb on - strand, within Psest_0117 at 114.777 kb on + strand, within Psest_0117 at 114.778 kb on - strand, within Psest_0117 at 114.778 kb on - strand, within Psest_0117 at 114.778 kb on - strand, within Psest_0117 at 114.778 kb on - strand, within Psest_0117 at 114.778 kb on - strand, within Psest_0117 at 114.907 kb on + strand, within Psest_0117 at 114.907 kb on + strand, within Psest_0117 at 114.907 kb on + strand, within Psest_0117 at 114.907 kb on + strand, within Psest_0117 at 114.907 kb on + strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117 at 114.908 kb on - strand, within Psest_0117
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.02 mg/ml remove 112,577 - Psest_0114 0.29 +1.4 112,789 + Psest_0114 0.66 +0.8 112,789 + Psest_0114 0.66 -1.0 112,968 + -0.9 112,969 - +0.7 112,969 - -0.8 113,112 + +1.1 113,112 + +0.9 113,112 + +1.4 113,112 + -1.6 113,113 - -1.6 113,113 - +0.0 113,113 - +1.7 113,113 - -0.5 113,113 - -0.4 113,384 + Psest_0115 0.78 +1.0 113,448 + -0.6 113,449 - -1.9 113,484 - +0.2 113,563 + Psest_0116 0.12 -1.3 113,563 + Psest_0116 0.12 -1.8 113,563 + Psest_0116 0.12 -1.2 113,563 + Psest_0116 0.12 +1.2 113,563 + Psest_0116 0.12 -2.3 113,563 + Psest_0116 0.12 +1.9 113,564 - Psest_0116 0.12 +0.4 113,564 - Psest_0116 0.12 -0.1 113,564 - Psest_0116 0.12 -0.6 113,564 - Psest_0116 0.12 -1.8 113,564 - Psest_0116 0.12 -0.1 113,564 - Psest_0116 0.12 -1.5 113,564 - Psest_0116 0.12 -1.3 113,564 - Psest_0116 0.12 -1.9 113,767 + Psest_0116 0.37 -1.0 113,767 + Psest_0116 0.37 -1.3 113,767 + Psest_0116 0.37 -0.8 113,767 + Psest_0116 0.37 -0.3 113,767 + Psest_0116 0.37 -0.9 113,767 + Psest_0116 0.37 +0.5 113,768 - Psest_0116 0.37 +0.0 113,768 - Psest_0116 0.37 +0.7 113,768 - Psest_0116 0.37 -2.4 113,768 - Psest_0116 0.37 -0.1 113,768 - Psest_0116 0.37 -1.3 113,768 - Psest_0116 0.37 -0.7 113,768 - Psest_0116 0.37 -1.0 113,888 - Psest_0116 0.52 +0.0 113,888 - Psest_0116 0.52 -1.2 114,505 - Psest_0117 0.28 -0.6 114,505 - Psest_0117 0.28 -2.1 114,505 - Psest_0117 0.28 -1.0 114,547 - Psest_0117 0.33 -0.2 114,603 + Psest_0117 0.40 -1.1 114,604 - Psest_0117 0.40 +0.0 114,604 - Psest_0117 0.40 -0.3 114,777 + Psest_0117 0.62 -2.7 114,778 - Psest_0117 0.62 -0.1 114,778 - Psest_0117 0.62 -0.1 114,778 - Psest_0117 0.62 +0.4 114,778 - Psest_0117 0.62 -0.7 114,778 - Psest_0117 0.62 -1.8 114,907 + Psest_0117 0.79 +0.3 114,907 + Psest_0117 0.79 -0.3 114,907 + Psest_0117 0.79 +0.9 114,907 + Psest_0117 0.79 +0.4 114,907 + Psest_0117 0.79 -1.3 114,908 - Psest_0117 0.79 -1.1 114,908 - Psest_0117 0.79 -2.2 114,908 - Psest_0117 0.79 +2.2 114,908 - Psest_0117 0.79 +1.5 114,908 - Psest_0117 0.79 -2.1 114,908 - Psest_0117 0.79 +0.2
Or see this region's nucleotide sequence