Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0994 and Psest_0995 are separated by 83 nucleotides Psest_0995 and Psest_0996 overlap by 4 nucleotides Psest_0996 and Psest_0997 are separated by 123 nucleotides Psest_0997 and Psest_0998 are separated by 1 nucleotides
Psest_0994: Psest_0994 - L-aspartate-alpha-decarboxylase, at 1,077,686 to 1,078,066
_0994
Psest_0995: Psest_0995 - pantoate--beta-alanine ligase, at 1,078,150 to 1,079,010
_0995
Psest_0996: Psest_0996 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,079,007 to 1,079,807
_0996
Psest_0997: Psest_0997 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase, at 1,079,931 to 1,080,458
_0997
Psest_0998: Psest_0998 - poly(A) polymerase, at 1,080,460 to 1,081,854
_0998
Position (kb)
1079
1080 Strain fitness (log2 ratio)
-2
-1
0
1 at 1079.895 kb on - strand at 1079.895 kb on - strand at 1079.895 kb on - strand at 1079.895 kb on - strand at 1079.895 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.915 kb on - strand at 1079.943 kb on + strand at 1079.944 kb on - strand at 1079.944 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 1,079,895 - +0.2 1,079,895 - -1.3 1,079,895 - -0.1 1,079,895 - -0.8 1,079,895 - -0.3 1,079,915 - -1.0 1,079,915 - -0.5 1,079,915 - -2.0 1,079,915 - -0.7 1,079,915 - -0.7 1,079,915 - -0.6 1,079,915 - +0.9 1,079,943 + -0.3 1,079,944 - -1.3 1,079,944 - -0.1
Or see this region's nucleotide sequence