Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0542 and Psest_0543 are separated by 80 nucleotides Psest_0543 and Psest_0544 are separated by 15 nucleotides Psest_0544 and Psest_0545 are separated by 76 nucleotides Psest_0545 and Psest_0546 overlap by 4 nucleotides
Psest_0542: Psest_0542 - AMP nucleosidase, at 597,893 to 599,368
_0542
Psest_0543: Psest_0543 - Protein of unknown function (DUF2789)., at 599,449 to 599,691
_0543
Psest_0544: Psest_0544 - Hydrogenase/urease accessory protein, at 599,707 to 600,279
_0544
Psest_0545: Psest_0545 - urease accessory protein UreG, at 600,356 to 600,970
_0545
Psest_0546: Psest_0546 - hypothetical protein, at 600,967 to 601,632
_0546
Position (kb)
599
600
601 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 598.798 kb on - strand, within Psest_0542 at 598.865 kb on + strand, within Psest_0542 at 598.865 kb on + strand, within Psest_0542 at 598.865 kb on + strand, within Psest_0542 at 598.866 kb on - strand, within Psest_0542 at 598.883 kb on + strand, within Psest_0542 at 598.883 kb on + strand, within Psest_0542 at 598.883 kb on + strand, within Psest_0542 at 598.883 kb on + strand, within Psest_0542 at 598.883 kb on + strand, within Psest_0542 at 598.884 kb on - strand, within Psest_0542 at 598.884 kb on - strand, within Psest_0542 at 598.884 kb on - strand, within Psest_0542 at 598.884 kb on - strand, within Psest_0542 at 598.884 kb on - strand, within Psest_0542 at 599.171 kb on + strand, within Psest_0542 at 599.246 kb on + strand at 599.246 kb on + strand at 599.247 kb on - strand at 599.247 kb on - strand at 599.247 kb on - strand at 599.408 kb on + strand at 599.409 kb on - strand at 599.415 kb on - strand at 599.415 kb on - strand at 599.415 kb on - strand at 599.415 kb on - strand at 599.475 kb on + strand, within Psest_0543 at 599.563 kb on + strand, within Psest_0543 at 600.124 kb on + strand, within Psest_0544 at 600.125 kb on - strand, within Psest_0544 at 600.271 kb on + strand at 600.271 kb on + strand at 600.271 kb on + strand at 600.271 kb on + strand at 600.606 kb on + strand, within Psest_0545 at 600.606 kb on + strand, within Psest_0545 at 600.606 kb on + strand, within Psest_0545 at 600.606 kb on + strand, within Psest_0545 at 600.606 kb on + strand, within Psest_0545 at 600.606 kb on + strand, within Psest_0545 at 600.606 kb on + strand, within Psest_0545 at 600.606 kb on + strand, within Psest_0545 at 600.606 kb on + strand, within Psest_0545 at 600.606 kb on + strand, within Psest_0545 at 600.606 kb on + strand, within Psest_0545 at 600.606 kb on + strand, within Psest_0545 at 600.606 kb on + strand, within Psest_0545 at 600.607 kb on - strand, within Psest_0545 at 600.607 kb on - strand, within Psest_0545 at 600.607 kb on - strand, within Psest_0545 at 600.607 kb on - strand, within Psest_0545 at 600.607 kb on - strand, within Psest_0545 at 600.607 kb on - strand, within Psest_0545 at 600.843 kb on + strand, within Psest_0545 at 600.843 kb on + strand, within Psest_0545 at 600.843 kb on + strand, within Psest_0545 at 600.843 kb on + strand, within Psest_0545 at 600.843 kb on + strand, within Psest_0545 at 600.843 kb on + strand, within Psest_0545 at 600.843 kb on + strand, within Psest_0545 at 600.843 kb on + strand, within Psest_0545 at 600.843 kb on + strand, within Psest_0545 at 600.843 kb on + strand, within Psest_0545 at 600.843 kb on + strand, within Psest_0545 at 600.844 kb on - strand, within Psest_0545 at 600.844 kb on - strand, within Psest_0545 at 600.844 kb on - strand, within Psest_0545 at 600.844 kb on - strand, within Psest_0545 at 600.844 kb on - strand, within Psest_0545 at 600.844 kb on - strand, within Psest_0545 at 600.844 kb on - strand, within Psest_0545 at 600.844 kb on - strand, within Psest_0545 at 600.844 kb on - strand, within Psest_0545 at 600.873 kb on + strand, within Psest_0545 at 601.060 kb on - strand, within Psest_0546 at 601.080 kb on + strand, within Psest_0546 at 601.099 kb on + strand, within Psest_0546 at 601.099 kb on + strand, within Psest_0546 at 601.168 kb on + strand, within Psest_0546 at 601.168 kb on + strand, within Psest_0546 at 601.168 kb on + strand, within Psest_0546 at 601.168 kb on + strand, within Psest_0546 at 601.168 kb on + strand, within Psest_0546 at 601.169 kb on - strand, within Psest_0546
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 598,798 - Psest_0542 0.61 +0.7 598,865 + Psest_0542 0.66 +0.5 598,865 + Psest_0542 0.66 +0.7 598,865 + Psest_0542 0.66 +1.9 598,866 - Psest_0542 0.66 +1.1 598,883 + Psest_0542 0.67 +0.1 598,883 + Psest_0542 0.67 +0.7 598,883 + Psest_0542 0.67 +2.1 598,883 + Psest_0542 0.67 -1.1 598,883 + Psest_0542 0.67 -0.4 598,884 - Psest_0542 0.67 +0.7 598,884 - Psest_0542 0.67 +0.4 598,884 - Psest_0542 0.67 +1.2 598,884 - Psest_0542 0.67 -0.0 598,884 - Psest_0542 0.67 +1.6 599,171 + Psest_0542 0.87 +1.4 599,246 + +4.3 599,246 + +1.7 599,247 - +0.5 599,247 - -0.3 599,247 - -0.3 599,408 + +0.9 599,409 - +2.1 599,415 - +0.4 599,415 - +3.0 599,415 - +2.0 599,415 - -0.0 599,475 + Psest_0543 0.11 +0.0 599,563 + Psest_0543 0.47 +3.0 600,124 + Psest_0544 0.73 -0.6 600,125 - Psest_0544 0.73 +1.0 600,271 + +0.9 600,271 + +0.4 600,271 + +0.7 600,271 + +1.2 600,606 + Psest_0545 0.41 +1.1 600,606 + Psest_0545 0.41 -0.0 600,606 + Psest_0545 0.41 +1.3 600,606 + Psest_0545 0.41 +0.4 600,606 + Psest_0545 0.41 +2.0 600,606 + Psest_0545 0.41 +0.7 600,606 + Psest_0545 0.41 +2.8 600,606 + Psest_0545 0.41 +1.1 600,606 + Psest_0545 0.41 -0.4 600,606 + Psest_0545 0.41 +0.5 600,606 + Psest_0545 0.41 +3.0 600,606 + Psest_0545 0.41 +1.0 600,606 + Psest_0545 0.41 +0.6 600,607 - Psest_0545 0.41 -0.3 600,607 - Psest_0545 0.41 +1.5 600,607 - Psest_0545 0.41 -0.8 600,607 - Psest_0545 0.41 +2.0 600,607 - Psest_0545 0.41 +1.3 600,607 - Psest_0545 0.41 +1.0 600,843 + Psest_0545 0.79 -0.7 600,843 + Psest_0545 0.79 +2.7 600,843 + Psest_0545 0.79 +0.3 600,843 + Psest_0545 0.79 -1.4 600,843 + Psest_0545 0.79 +2.0 600,843 + Psest_0545 0.79 +1.7 600,843 + Psest_0545 0.79 +1.9 600,843 + Psest_0545 0.79 +0.7 600,843 + Psest_0545 0.79 +1.4 600,843 + Psest_0545 0.79 +1.3 600,843 + Psest_0545 0.79 +0.4 600,844 - Psest_0545 0.79 -1.9 600,844 - Psest_0545 0.79 +1.1 600,844 - Psest_0545 0.79 -0.8 600,844 - Psest_0545 0.79 -0.0 600,844 - Psest_0545 0.79 -1.3 600,844 - Psest_0545 0.79 +1.2 600,844 - Psest_0545 0.79 -1.8 600,844 - Psest_0545 0.79 -1.9 600,844 - Psest_0545 0.79 +0.1 600,873 + Psest_0545 0.84 +0.1 601,060 - Psest_0546 0.14 -0.5 601,080 + Psest_0546 0.17 -0.0 601,099 + Psest_0546 0.20 +1.9 601,099 + Psest_0546 0.20 +0.4 601,168 + Psest_0546 0.30 +1.4 601,168 + Psest_0546 0.30 +2.1 601,168 + Psest_0546 0.30 +2.1 601,168 + Psest_0546 0.30 +1.3 601,168 + Psest_0546 0.30 +1.2 601,169 - Psest_0546 0.30 +1.4
Or see this region's nucleotide sequence