Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0535 and Psest_0536 are separated by 55 nucleotides Psest_0536 and Psest_0537 are separated by 14 nucleotides
Psest_0535: Psest_0535 - glutamate-1-semialdehyde-2,1-aminomutase, at 587,985 to 589,268
_0535
Psest_0536: Psest_0536 - thiamine-phosphate pyrophosphorylase, at 589,324 to 589,965
_0536
Psest_0537: Psest_0537 - Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase, at 589,980 to 590,774
_0537
Position (kb) 589
590 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 589.332 kb on + strand at 589.333 kb on - strand at 589.333 kb on - strand at 589.584 kb on - strand, within Psest_0536 at 589.584 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.887 kb on - strand, within Psest_0536 at 589.935 kb on - strand at 590.014 kb on + strand at 590.014 kb on + strand at 590.014 kb on + strand at 590.014 kb on + strand at 590.015 kb on - strand at 590.015 kb on - strand at 590.015 kb on - strand at 590.015 kb on - strand at 590.015 kb on - strand at 590.015 kb on - strand at 590.015 kb on - strand at 590.015 kb on - strand at 590.062 kb on + strand, within Psest_0537 at 590.063 kb on - strand, within Psest_0537 at 590.210 kb on - strand, within Psest_0537 at 590.396 kb on - strand, within Psest_0537 at 590.788 kb on - strand at 590.788 kb on - strand at 590.910 kb on + strand at 590.911 kb on - strand at 590.911 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 589,332 + -0.3 589,333 - +2.8 589,333 - +0.1 589,584 - Psest_0536 0.40 -1.3 589,584 - Psest_0536 0.40 -1.3 589,887 - Psest_0536 0.88 +0.2 589,887 - Psest_0536 0.88 -3.6 589,887 - Psest_0536 0.88 +0.7 589,887 - Psest_0536 0.88 -0.4 589,887 - Psest_0536 0.88 +0.0 589,887 - Psest_0536 0.88 +0.4 589,887 - Psest_0536 0.88 -1.6 589,887 - Psest_0536 0.88 -0.6 589,887 - Psest_0536 0.88 +0.7 589,887 - Psest_0536 0.88 -1.6 589,935 - +0.4 590,014 + -0.1 590,014 + -1.4 590,014 + +0.7 590,014 + +0.4 590,015 - +0.5 590,015 - -0.4 590,015 - +0.4 590,015 - -1.0 590,015 - -2.4 590,015 - -0.9 590,015 - +0.4 590,015 - +1.4 590,062 + Psest_0537 0.10 -1.4 590,063 - Psest_0537 0.10 -1.2 590,210 - Psest_0537 0.29 +0.4 590,396 - Psest_0537 0.52 -1.7 590,788 - +1.3 590,788 - +0.1 590,910 + +0.4 590,911 - +3.5 590,911 - +3.8
Or see this region's nucleotide sequence