Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0507 and Psest_0508 are separated by 216 nucleotides Psest_0508 and Psest_0509 overlap by 1 nucleotides Psest_0509 and Psest_0510 are separated by 15 nucleotides
Psest_0507: Psest_0507 - Uncharacterized membrane-associated protein, at 560,440 to 561,759
_0507
Psest_0508: Psest_0508 - gamma-glutamyl phosphate reductase, at 561,976 to 563,241
_0508
Psest_0509: Psest_0509 - nicotinate (nicotinamide) nucleotide adenylyltransferase, at 563,241 to 563,900
_0509
Psest_0510: Psest_0510 - iojap-like ribosome-associated protein, at 563,916 to 564,275
_0510
Position (kb) 561
562
563
564 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 561.211 kb on + strand, within Psest_0507 at 561.212 kb on - strand, within Psest_0507 at 561.212 kb on - strand, within Psest_0507 at 561.288 kb on - strand, within Psest_0507 at 561.288 kb on - strand, within Psest_0507 at 561.288 kb on - strand, within Psest_0507 at 561.440 kb on + strand, within Psest_0507 at 561.440 kb on + strand, within Psest_0507 at 561.440 kb on + strand, within Psest_0507 at 561.441 kb on - strand, within Psest_0507 at 561.441 kb on - strand, within Psest_0507 at 561.763 kb on + strand at 561.800 kb on + strand at 561.800 kb on + strand at 561.801 kb on - strand at 561.801 kb on - strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.935 kb on + strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.936 kb on - strand at 561.937 kb on + strand at 561.969 kb on + strand at 561.969 kb on + strand at 561.969 kb on + strand at 561.969 kb on + strand at 561.997 kb on + strand at 561.997 kb on + strand at 561.997 kb on + strand at 562.248 kb on + strand, within Psest_0508 at 562.248 kb on + strand, within Psest_0508 at 562.288 kb on + strand, within Psest_0508 at 562.288 kb on + strand, within Psest_0508 at 562.288 kb on + strand, within Psest_0508 at 562.351 kb on + strand, within Psest_0508 at 562.351 kb on + strand, within Psest_0508 at 562.351 kb on + strand, within Psest_0508 at 562.351 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.678 kb on + strand, within Psest_0508 at 562.924 kb on + strand, within Psest_0508 at 562.924 kb on + strand, within Psest_0508 at 563.206 kb on + strand at 563.206 kb on + strand at 563.206 kb on + strand at 563.206 kb on + strand at 563.206 kb on + strand at 563.206 kb on + strand at 564.021 kb on + strand, within Psest_0510 at 564.021 kb on + strand, within Psest_0510 at 564.021 kb on + strand, within Psest_0510 at 564.021 kb on + strand, within Psest_0510 at 564.021 kb on + strand, within Psest_0510 at 564.021 kb on + strand, within Psest_0510 at 564.021 kb on + strand, within Psest_0510 at 564.021 kb on + strand, within Psest_0510 at 564.058 kb on + strand, within Psest_0510 at 564.058 kb on + strand, within Psest_0510 at 564.058 kb on + strand, within Psest_0510 at 564.207 kb on + strand, within Psest_0510 at 564.207 kb on + strand, within Psest_0510
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 561,211 + Psest_0507 0.58 +1.2 561,212 - Psest_0507 0.58 +1.2 561,212 - Psest_0507 0.58 +0.3 561,288 - Psest_0507 0.64 -0.1 561,288 - Psest_0507 0.64 +1.4 561,288 - Psest_0507 0.64 +0.8 561,440 + Psest_0507 0.76 +1.1 561,440 + Psest_0507 0.76 +0.1 561,440 + Psest_0507 0.76 -1.0 561,441 - Psest_0507 0.76 +0.2 561,441 - Psest_0507 0.76 +1.4 561,763 + -0.3 561,800 + +1.1 561,800 + +1.6 561,801 - -1.3 561,801 - -0.1 561,935 + +0.4 561,935 + +1.1 561,935 + +0.8 561,935 + +0.9 561,935 + +0.6 561,935 + +0.1 561,935 + +1.9 561,935 + +0.5 561,935 + -0.6 561,935 + +0.1 561,935 + +1.6 561,935 + +0.7 561,935 + +0.2 561,935 + -0.6 561,935 + +1.1 561,935 + -0.1 561,935 + +0.8 561,935 + +0.5 561,936 - -4.5 561,936 - -1.4 561,936 - -1.2 561,936 - -1.7 561,936 - -1.9 561,936 - -0.6 561,936 - -0.4 561,936 - -3.3 561,936 - -0.6 561,936 - -1.5 561,936 - -2.5 561,936 - -1.7 561,936 - +1.7 561,936 - -2.1 561,936 - -1.3 561,936 - -2.1 561,936 - +0.8 561,936 - -0.5 561,936 - -1.0 561,936 - -3.4 561,936 - -2.8 561,937 + -0.5 561,969 + -0.8 561,969 + +0.7 561,969 + +1.5 561,969 + -0.5 561,997 + +1.5 561,997 + -1.0 561,997 + -0.8 562,248 + Psest_0508 0.21 -1.3 562,248 + Psest_0508 0.21 -2.7 562,288 + Psest_0508 0.25 -1.0 562,288 + Psest_0508 0.25 -3.7 562,288 + Psest_0508 0.25 -1.0 562,351 + Psest_0508 0.30 -2.6 562,351 + Psest_0508 0.30 -1.5 562,351 + Psest_0508 0.30 -3.5 562,351 + Psest_0508 0.30 -2.5 562,678 + Psest_0508 0.55 -1.2 562,678 + Psest_0508 0.55 -0.4 562,678 + Psest_0508 0.55 -1.9 562,678 + Psest_0508 0.55 -2.0 562,678 + Psest_0508 0.55 -0.4 562,678 + Psest_0508 0.55 -2.2 562,678 + Psest_0508 0.55 -2.7 562,678 + Psest_0508 0.55 -4.1 562,678 + Psest_0508 0.55 -0.3 562,678 + Psest_0508 0.55 -0.4 562,678 + Psest_0508 0.55 -2.6 562,678 + Psest_0508 0.55 -3.3 562,924 + Psest_0508 0.75 -0.1 562,924 + Psest_0508 0.75 -2.1 563,206 + -3.0 563,206 + -3.7 563,206 + -0.3 563,206 + -0.5 563,206 + -0.3 563,206 + -0.9 564,021 + Psest_0510 0.29 +2.9 564,021 + Psest_0510 0.29 +0.6 564,021 + Psest_0510 0.29 -1.6 564,021 + Psest_0510 0.29 -0.3 564,021 + Psest_0510 0.29 +2.1 564,021 + Psest_0510 0.29 +0.6 564,021 + Psest_0510 0.29 +0.1 564,021 + Psest_0510 0.29 +0.9 564,058 + Psest_0510 0.39 +0.9 564,058 + Psest_0510 0.39 +2.1 564,058 + Psest_0510 0.39 +0.9 564,207 + Psest_0510 0.81 +0.6 564,207 + Psest_0510 0.81 +1.3
Or see this region's nucleotide sequence