Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0473 and Psest_0474 overlap by 8 nucleotides Psest_0474 and Psest_0475 are separated by 112 nucleotides
Psest_0473: Psest_0473 - Glycerol-3-phosphate dehydrogenase, at 521,763 to 522,938
_0473
Psest_0474: Psest_0474 - Membrane transporters of cations and cationic drugs, at 522,931 to 523,329
_0474
Psest_0475: Psest_0475 - 3-deoxy-D-manno-octulosonic-acid transferase, at 523,442 to 524,710
_0475
Position (kb) 522
523
524 Strain fitness (log2 ratio) -2
-1
0
1
2 at 522.952 kb on - strand at 522.973 kb on - strand, within Psest_0474 at 522.973 kb on - strand, within Psest_0474 at 522.973 kb on - strand, within Psest_0474 at 522.973 kb on - strand, within Psest_0474 at 522.973 kb on - strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.974 kb on + strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 522.975 kb on - strand, within Psest_0474 at 523.151 kb on + strand, within Psest_0474 at 523.151 kb on + strand, within Psest_0474 at 523.313 kb on + strand at 523.313 kb on + strand at 523.314 kb on - strand at 523.345 kb on - strand at 523.457 kb on + strand at 523.457 kb on + strand at 523.457 kb on + strand at 523.457 kb on + strand at 523.457 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 522,952 - +0.5 522,973 - Psest_0474 0.11 -0.3 522,973 - Psest_0474 0.11 +1.0 522,973 - Psest_0474 0.11 +0.8 522,973 - Psest_0474 0.11 -0.1 522,973 - Psest_0474 0.11 +0.6 522,974 + Psest_0474 0.11 +0.5 522,974 + Psest_0474 0.11 +1.0 522,974 + Psest_0474 0.11 +0.2 522,974 + Psest_0474 0.11 -0.7 522,974 + Psest_0474 0.11 +0.4 522,975 - Psest_0474 0.11 +1.1 522,975 - Psest_0474 0.11 +0.6 522,975 - Psest_0474 0.11 +1.6 522,975 - Psest_0474 0.11 +1.1 522,975 - Psest_0474 0.11 +1.0 522,975 - Psest_0474 0.11 +1.7 522,975 - Psest_0474 0.11 +1.7 522,975 - Psest_0474 0.11 +0.3 523,151 + Psest_0474 0.55 +0.8 523,151 + Psest_0474 0.55 -2.6 523,313 + +0.2 523,313 + +1.1 523,314 - -1.6 523,345 - -0.5 523,457 + +0.6 523,457 + +0.3 523,457 + +1.8 523,457 + +1.3 523,457 + +2.4
Or see this region's nucleotide sequence