Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0413 and Psest_0414 overlap by 8 nucleotides Psest_0414 and Psest_0415 are separated by 1 nucleotides Psest_0415 and Psest_0416 are separated by 85 nucleotides Psest_0416 and Psest_0417 are separated by 360 nucleotides
Psest_0413: Psest_0413 - Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily), at 448,933 to 449,658
_0413
Psest_0414: Psest_0414 - biotin biosynthesis protein BioC, at 449,651 to 450,445
_0414
Psest_0415: Psest_0415 - dethiobiotin synthase, at 450,447 to 451,133
_0415
Psest_0416: Psest_0416 - hypothetical protein, at 451,219 to 451,467
_0416
Psest_0417: Psest_0417 - Acyl-CoA dehydrogenases, at 451,828 to 453,633
_0417
Position (kb) 450
451
452 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 449.461 kb on + strand, within Psest_0413 at 449.462 kb on - strand, within Psest_0413 at 449.462 kb on - strand, within Psest_0413 at 449.721 kb on - strand at 449.721 kb on - strand at 449.721 kb on - strand at 449.993 kb on + strand, within Psest_0414 at 449.993 kb on + strand, within Psest_0414 at 449.994 kb on - strand, within Psest_0414 at 449.994 kb on - strand, within Psest_0414 at 449.994 kb on - strand, within Psest_0414 at 450.182 kb on + strand, within Psest_0414 at 450.182 kb on + strand, within Psest_0414 at 450.182 kb on + strand, within Psest_0414 at 450.182 kb on + strand, within Psest_0414 at 450.183 kb on - strand, within Psest_0414 at 450.183 kb on - strand, within Psest_0414 at 450.183 kb on - strand, within Psest_0414 at 450.183 kb on - strand, within Psest_0414 at 450.407 kb on + strand at 450.407 kb on + strand at 450.407 kb on + strand at 450.407 kb on + strand at 450.408 kb on - strand at 450.408 kb on - strand at 450.408 kb on - strand at 450.408 kb on - strand at 450.420 kb on - strand at 450.420 kb on - strand at 450.557 kb on - strand, within Psest_0415 at 450.794 kb on - strand, within Psest_0415 at 450.837 kb on + strand, within Psest_0415 at 450.837 kb on + strand, within Psest_0415 at 450.837 kb on + strand, within Psest_0415 at 450.838 kb on - strand, within Psest_0415 at 450.922 kb on + strand, within Psest_0415 at 450.922 kb on + strand, within Psest_0415 at 450.923 kb on - strand, within Psest_0415 at 450.923 kb on - strand, within Psest_0415 at 450.923 kb on - strand, within Psest_0415 at 450.923 kb on - strand, within Psest_0415 at 451.233 kb on + strand at 451.261 kb on + strand, within Psest_0416 at 451.261 kb on + strand, within Psest_0416 at 451.261 kb on + strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.262 kb on - strand, within Psest_0416 at 451.633 kb on - strand at 451.633 kb on - strand at 451.633 kb on - strand at 451.633 kb on - strand at 451.671 kb on + strand at 451.671 kb on + strand at 451.672 kb on - strand at 451.672 kb on - strand at 451.713 kb on + strand at 451.713 kb on + strand at 451.713 kb on + strand at 451.809 kb on + strand at 451.822 kb on + strand at 451.837 kb on + strand at 451.837 kb on + strand at 451.838 kb on - strand at 451.868 kb on + strand at 451.868 kb on + strand at 451.869 kb on - strand at 451.889 kb on - strand at 451.901 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 449,461 + Psest_0413 0.73 +0.2 449,462 - Psest_0413 0.73 +0.1 449,462 - Psest_0413 0.73 -1.2 449,721 - -1.3 449,721 - -0.7 449,721 - -1.1 449,993 + Psest_0414 0.43 -1.2 449,993 + Psest_0414 0.43 -1.4 449,994 - Psest_0414 0.43 -2.4 449,994 - Psest_0414 0.43 -1.1 449,994 - Psest_0414 0.43 +0.2 450,182 + Psest_0414 0.67 -1.2 450,182 + Psest_0414 0.67 +0.0 450,182 + Psest_0414 0.67 -0.4 450,182 + Psest_0414 0.67 -0.8 450,183 - Psest_0414 0.67 +1.4 450,183 - Psest_0414 0.67 +0.4 450,183 - Psest_0414 0.67 +0.3 450,183 - Psest_0414 0.67 -1.6 450,407 + -1.7 450,407 + -1.0 450,407 + -1.5 450,407 + -0.8 450,408 - -0.1 450,408 - -0.9 450,408 - -1.0 450,408 - -1.5 450,420 - -1.2 450,420 - -1.4 450,557 - Psest_0415 0.16 -2.5 450,794 - Psest_0415 0.51 -2.6 450,837 + Psest_0415 0.57 -2.6 450,837 + Psest_0415 0.57 -1.6 450,837 + Psest_0415 0.57 -0.0 450,838 - Psest_0415 0.57 -1.6 450,922 + Psest_0415 0.69 +0.4 450,922 + Psest_0415 0.69 -1.4 450,923 - Psest_0415 0.69 -0.6 450,923 - Psest_0415 0.69 +1.0 450,923 - Psest_0415 0.69 +0.2 450,923 - Psest_0415 0.69 -1.6 451,233 + -1.3 451,261 + Psest_0416 0.17 -1.0 451,261 + Psest_0416 0.17 +0.7 451,261 + Psest_0416 0.17 -0.6 451,262 - Psest_0416 0.17 +0.1 451,262 - Psest_0416 0.17 -1.4 451,262 - Psest_0416 0.17 -0.3 451,262 - Psest_0416 0.17 +2.0 451,262 - Psest_0416 0.17 -0.3 451,262 - Psest_0416 0.17 +0.3 451,262 - Psest_0416 0.17 -0.1 451,633 - -0.3 451,633 - -0.9 451,633 - +1.4 451,633 - +0.3 451,671 + -0.9 451,671 + -0.6 451,672 - -0.1 451,672 - -0.1 451,713 + -0.4 451,713 + -2.8 451,713 + -0.5 451,809 + +0.7 451,822 + +3.7 451,837 + +0.4 451,837 + +2.0 451,838 - +0.4 451,868 + +1.4 451,868 + +1.9 451,869 - +0.3 451,889 - +2.7 451,901 - +2.5
Or see this region's nucleotide sequence