Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0409 and Psest_0410 are separated by 71 nucleotides Psest_0410 and Psest_0411 are separated by 93 nucleotides Psest_0411 and Psest_0412 are separated by 107 nucleotides
Psest_0409: Psest_0409 - Putative homoserine kinase type II (protein kinase fold), at 444,755 to 445,729
_0409
Psest_0410: Psest_0410 - Predicted amidophosphoribosyltransferases, at 445,801 to 446,505
_0410
Psest_0411: Psest_0411 - biotin synthetase, at 446,599 to 447,654
_0411
Psest_0412: Psest_0412 - 8-amino-7-oxononanoate synthase, at 447,762 to 448,940
_0412
Position (kb)
446
447
448 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 445.615 kb on - strand, within Psest_0409 at 445.711 kb on + strand at 445.747 kb on - strand at 445.760 kb on + strand at 445.777 kb on + strand at 445.778 kb on - strand at 445.778 kb on - strand at 445.835 kb on + strand at 445.835 kb on + strand at 445.836 kb on - strand at 445.836 kb on - strand at 446.266 kb on - strand, within Psest_0410 at 446.318 kb on + strand, within Psest_0410 at 446.319 kb on - strand, within Psest_0410 at 446.430 kb on + strand, within Psest_0410 at 446.430 kb on + strand, within Psest_0410 at 446.431 kb on - strand, within Psest_0410 at 446.431 kb on - strand, within Psest_0410 at 446.431 kb on - strand, within Psest_0410 at 446.431 kb on - strand, within Psest_0410 at 446.431 kb on - strand, within Psest_0410 at 446.581 kb on + strand at 446.581 kb on + strand at 446.581 kb on + strand at 446.581 kb on + strand at 446.582 kb on - strand at 446.582 kb on - strand at 446.585 kb on + strand at 446.585 kb on + strand at 446.585 kb on + strand at 446.585 kb on + strand at 446.791 kb on + strand, within Psest_0411 at 446.791 kb on + strand, within Psest_0411 at 446.791 kb on + strand, within Psest_0411 at 446.791 kb on + strand, within Psest_0411 at 446.791 kb on + strand, within Psest_0411 at 446.791 kb on + strand, within Psest_0411 at 446.792 kb on - strand, within Psest_0411 at 446.813 kb on - strand, within Psest_0411 at 446.813 kb on - strand, within Psest_0411 at 446.813 kb on - strand, within Psest_0411 at 446.950 kb on + strand, within Psest_0411 at 446.951 kb on - strand, within Psest_0411 at 446.951 kb on - strand, within Psest_0411 at 447.062 kb on - strand, within Psest_0411 at 447.064 kb on + strand, within Psest_0411 at 447.064 kb on + strand, within Psest_0411 at 447.064 kb on + strand, within Psest_0411 at 447.064 kb on + strand, within Psest_0411 at 447.064 kb on + strand, within Psest_0411 at 447.065 kb on - strand, within Psest_0411 at 447.065 kb on - strand, within Psest_0411 at 447.065 kb on - strand, within Psest_0411 at 447.065 kb on - strand, within Psest_0411 at 447.081 kb on + strand, within Psest_0411 at 447.094 kb on + strand, within Psest_0411 at 447.094 kb on + strand, within Psest_0411 at 447.095 kb on - strand, within Psest_0411 at 447.095 kb on - strand, within Psest_0411 at 447.095 kb on - strand, within Psest_0411 at 447.097 kb on + strand, within Psest_0411 at 447.124 kb on + strand, within Psest_0411 at 447.124 kb on + strand, within Psest_0411 at 447.124 kb on + strand, within Psest_0411 at 447.152 kb on - strand, within Psest_0411 at 447.152 kb on - strand, within Psest_0411 at 447.152 kb on - strand, within Psest_0411 at 447.152 kb on - strand, within Psest_0411 at 447.152 kb on - strand, within Psest_0411 at 447.152 kb on - strand, within Psest_0411 at 447.152 kb on - strand, within Psest_0411 at 447.637 kb on + strand at 447.637 kb on + strand at 447.637 kb on + strand at 447.637 kb on + strand at 447.637 kb on + strand at 447.637 kb on + strand at 447.638 kb on - strand at 447.640 kb on - strand at 447.814 kb on - strand at 447.900 kb on + strand, within Psest_0412 at 447.901 kb on - strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.081 kb on - strand, within Psest_0412 at 448.081 kb on - strand, within Psest_0412 at 448.081 kb on - strand, within Psest_0412 at 448.081 kb on - strand, within Psest_0412 at 448.082 kb on + strand, within Psest_0412 at 448.083 kb on - strand, within Psest_0412 at 448.282 kb on + strand, within Psest_0412 at 448.545 kb on + strand, within Psest_0412 at 448.545 kb on + strand, within Psest_0412 at 448.545 kb on + strand, within Psest_0412 at 448.545 kb on + strand, within Psest_0412 at 448.546 kb on - strand, within Psest_0412 at 448.546 kb on - strand, within Psest_0412 at 448.546 kb on - strand, within Psest_0412 at 448.551 kb on + strand, within Psest_0412 at 448.551 kb on + strand, within Psest_0412 at 448.551 kb on + strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.613 kb on + strand, within Psest_0412 at 448.614 kb on - strand, within Psest_0412
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 445,615 - Psest_0409 0.88 +0.4 445,711 + +0.9 445,747 - -1.1 445,760 + +0.4 445,777 + -1.3 445,778 - +0.3 445,778 - +2.8 445,835 + +0.2 445,835 + +2.2 445,836 - +1.8 445,836 - -0.3 446,266 - Psest_0410 0.66 +1.1 446,318 + Psest_0410 0.73 -1.9 446,319 - Psest_0410 0.73 +0.5 446,430 + Psest_0410 0.89 -0.9 446,430 + Psest_0410 0.89 -0.0 446,431 - Psest_0410 0.89 -0.6 446,431 - Psest_0410 0.89 +0.5 446,431 - Psest_0410 0.89 +0.6 446,431 - Psest_0410 0.89 +0.1 446,431 - Psest_0410 0.89 -0.6 446,581 + +0.6 446,581 + +0.6 446,581 + +0.9 446,581 + +1.2 446,582 - +0.4 446,582 - -1.5 446,585 + -0.4 446,585 + -1.5 446,585 + +1.0 446,585 + +0.4 446,791 + Psest_0411 0.18 -2.2 446,791 + Psest_0411 0.18 -1.3 446,791 + Psest_0411 0.18 -3.9 446,791 + Psest_0411 0.18 -0.3 446,791 + Psest_0411 0.18 -1.0 446,791 + Psest_0411 0.18 -1.7 446,792 - Psest_0411 0.18 -0.8 446,813 - Psest_0411 0.20 -3.1 446,813 - Psest_0411 0.20 -2.0 446,813 - Psest_0411 0.20 -1.2 446,950 + Psest_0411 0.33 -2.5 446,951 - Psest_0411 0.33 -2.4 446,951 - Psest_0411 0.33 -1.7 447,062 - Psest_0411 0.44 -3.3 447,064 + Psest_0411 0.44 -1.9 447,064 + Psest_0411 0.44 -2.1 447,064 + Psest_0411 0.44 -3.8 447,064 + Psest_0411 0.44 -2.4 447,064 + Psest_0411 0.44 -1.5 447,065 - Psest_0411 0.44 -3.1 447,065 - Psest_0411 0.44 -2.6 447,065 - Psest_0411 0.44 -1.4 447,065 - Psest_0411 0.44 -2.0 447,081 + Psest_0411 0.46 -2.3 447,094 + Psest_0411 0.47 -2.4 447,094 + Psest_0411 0.47 -2.3 447,095 - Psest_0411 0.47 -1.2 447,095 - Psest_0411 0.47 -3.0 447,095 - Psest_0411 0.47 -3.0 447,097 + Psest_0411 0.47 -2.8 447,124 + Psest_0411 0.50 -3.6 447,124 + Psest_0411 0.50 -2.1 447,124 + Psest_0411 0.50 -1.1 447,152 - Psest_0411 0.52 -3.0 447,152 - Psest_0411 0.52 -0.3 447,152 - Psest_0411 0.52 -2.0 447,152 - Psest_0411 0.52 -1.4 447,152 - Psest_0411 0.52 -0.5 447,152 - Psest_0411 0.52 -2.1 447,152 - Psest_0411 0.52 -1.3 447,637 + +0.5 447,637 + +0.7 447,637 + +1.0 447,637 + +0.1 447,637 + +0.5 447,637 + +0.6 447,638 - -0.3 447,640 - -2.3 447,814 - -0.9 447,900 + Psest_0412 0.12 -0.9 447,901 - Psest_0412 0.12 -1.2 448,080 + Psest_0412 0.27 -0.6 448,080 + Psest_0412 0.27 -2.0 448,080 + Psest_0412 0.27 -0.2 448,080 + Psest_0412 0.27 -1.4 448,080 + Psest_0412 0.27 -0.6 448,080 + Psest_0412 0.27 -1.3 448,080 + Psest_0412 0.27 +0.7 448,081 - Psest_0412 0.27 +0.0 448,081 - Psest_0412 0.27 -2.4 448,081 - Psest_0412 0.27 -1.2 448,081 - Psest_0412 0.27 -1.2 448,082 + Psest_0412 0.27 -1.5 448,083 - Psest_0412 0.27 +1.0 448,282 + Psest_0412 0.44 +0.3 448,545 + Psest_0412 0.66 -0.6 448,545 + Psest_0412 0.66 -1.9 448,545 + Psest_0412 0.66 -0.1 448,545 + Psest_0412 0.66 -2.1 448,546 - Psest_0412 0.66 -0.8 448,546 - Psest_0412 0.66 -1.9 448,546 - Psest_0412 0.66 -0.9 448,551 + Psest_0412 0.67 -0.9 448,551 + Psest_0412 0.67 -2.3 448,551 + Psest_0412 0.67 -0.1 448,552 - Psest_0412 0.67 -1.2 448,552 - Psest_0412 0.67 -2.9 448,552 - Psest_0412 0.67 -2.6 448,552 - Psest_0412 0.67 -0.6 448,552 - Psest_0412 0.67 +0.4 448,552 - Psest_0412 0.67 -3.3 448,552 - Psest_0412 0.67 -1.0 448,552 - Psest_0412 0.67 -2.2 448,552 - Psest_0412 0.67 -0.3 448,552 - Psest_0412 0.67 -2.3 448,552 - Psest_0412 0.67 -2.0 448,552 - Psest_0412 0.67 -1.7 448,552 - Psest_0412 0.67 -1.1 448,552 - Psest_0412 0.67 -0.5 448,552 - Psest_0412 0.67 -1.7 448,613 + Psest_0412 0.72 -1.5 448,614 - Psest_0412 0.72 -0.0
Or see this region's nucleotide sequence