Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_4132 and Psest_4133 are separated by 315 nucleotides Psest_4133 and Psest_4134 are separated by 2 nucleotides Psest_4134 and Psest_4135 are separated by 88 nucleotides
Psest_4132: Psest_4132 - alpha-ketoglutarate DNA-binding response regulator (mifR) (from data) , at 4,312,452 to 4,313,795
_4132
Psest_4133: Psest_4133 - RNA polymerase sigma factor, sigma-70 family, at 4,314,111 to 4,315,352
_4133
Psest_4134: Psest_4134 - Uncharacterized protein conserved in bacteria, at 4,315,355 to 4,315,711
_4134
Psest_4135: Psest_4135 - Ketosteroid isomerase homolog, at 4,315,800 to 4,316,258
_4135
Position (kb)
4314
4315
4316 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4313.255 kb on - strand, within Psest_4132 at 4313.384 kb on + strand, within Psest_4132 at 4313.384 kb on + strand, within Psest_4132 at 4313.385 kb on - strand, within Psest_4132 at 4313.385 kb on - strand, within Psest_4132 at 4313.766 kb on + strand at 4313.766 kb on + strand at 4313.766 kb on + strand at 4313.766 kb on + strand at 4313.766 kb on + strand at 4313.767 kb on - strand at 4313.767 kb on - strand at 4313.767 kb on - strand at 4313.767 kb on - strand at 4313.767 kb on - strand at 4313.789 kb on + strand at 4314.021 kb on + strand at 4314.022 kb on - strand at 4314.053 kb on - strand at 4314.053 kb on - strand at 4314.053 kb on - strand at 4314.053 kb on - strand at 4314.213 kb on - strand at 4314.213 kb on - strand at 4314.213 kb on - strand at 4314.213 kb on - strand at 4314.262 kb on + strand, within Psest_4133 at 4314.397 kb on + strand, within Psest_4133 at 4314.398 kb on - strand, within Psest_4133 at 4314.577 kb on - strand, within Psest_4133 at 4314.757 kb on + strand, within Psest_4133 at 4314.758 kb on - strand, within Psest_4133 at 4314.758 kb on - strand, within Psest_4133 at 4314.758 kb on - strand, within Psest_4133 at 4314.758 kb on - strand, within Psest_4133 at 4314.778 kb on + strand, within Psest_4133 at 4314.778 kb on + strand, within Psest_4133 at 4314.778 kb on + strand, within Psest_4133 at 4314.779 kb on - strand, within Psest_4133 at 4314.779 kb on - strand, within Psest_4133 at 4314.779 kb on - strand, within Psest_4133 at 4314.779 kb on - strand, within Psest_4133 at 4314.882 kb on - strand, within Psest_4133 at 4315.183 kb on - strand, within Psest_4133 at 4315.306 kb on + strand at 4315.467 kb on + strand, within Psest_4134 at 4315.467 kb on + strand, within Psest_4134 at 4315.467 kb on + strand, within Psest_4134 at 4315.468 kb on - strand, within Psest_4134 at 4315.468 kb on - strand, within Psest_4134 at 4315.687 kb on - strand at 4315.687 kb on - strand at 4315.689 kb on + strand at 4315.689 kb on + strand at 4315.689 kb on + strand at 4315.689 kb on + strand at 4315.689 kb on + strand at 4315.689 kb on + strand at 4315.690 kb on - strand at 4315.690 kb on - strand at 4315.690 kb on - strand at 4315.690 kb on - strand at 4315.690 kb on - strand at 4315.690 kb on - strand at 4315.704 kb on + strand at 4315.704 kb on + strand at 4315.704 kb on + strand at 4315.705 kb on - strand at 4315.705 kb on - strand at 4315.705 kb on - strand at 4315.705 kb on - strand at 4315.746 kb on + strand at 4315.747 kb on - strand at 4315.856 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.143 kb on - strand, within Psest_4135 at 4316.143 kb on - strand, within Psest_4135 at 4316.143 kb on - strand, within Psest_4135 at 4316.143 kb on - strand, within Psest_4135 at 4316.299 kb on + strand at 4316.299 kb on + strand at 4316.299 kb on + strand at 4316.299 kb on + strand at 4316.299 kb on + strand at 4316.300 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 4,313,255 - Psest_4132 0.60 +1.7 4,313,384 + Psest_4132 0.69 +0.7 4,313,384 + Psest_4132 0.69 +0.7 4,313,385 - Psest_4132 0.69 +0.3 4,313,385 - Psest_4132 0.69 +0.4 4,313,766 + +0.3 4,313,766 + -1.2 4,313,766 + -0.5 4,313,766 + +1.0 4,313,766 + +0.5 4,313,767 - +1.6 4,313,767 - +1.1 4,313,767 - +1.7 4,313,767 - +0.5 4,313,767 - +1.4 4,313,789 + +0.3 4,314,021 + -1.9 4,314,022 - +2.0 4,314,053 - +0.0 4,314,053 - +1.0 4,314,053 - +0.3 4,314,053 - +0.7 4,314,213 - +3.1 4,314,213 - +2.2 4,314,213 - +1.3 4,314,213 - +1.6 4,314,262 + Psest_4133 0.12 +0.8 4,314,397 + Psest_4133 0.23 +0.6 4,314,398 - Psest_4133 0.23 +0.2 4,314,577 - Psest_4133 0.38 +2.2 4,314,757 + Psest_4133 0.52 +2.0 4,314,758 - Psest_4133 0.52 +1.3 4,314,758 - Psest_4133 0.52 +0.8 4,314,758 - Psest_4133 0.52 +0.6 4,314,758 - Psest_4133 0.52 +0.4 4,314,778 + Psest_4133 0.54 +1.1 4,314,778 + Psest_4133 0.54 +0.3 4,314,778 + Psest_4133 0.54 +1.4 4,314,779 - Psest_4133 0.54 +0.9 4,314,779 - Psest_4133 0.54 +0.8 4,314,779 - Psest_4133 0.54 +0.9 4,314,779 - Psest_4133 0.54 +0.6 4,314,882 - Psest_4133 0.62 +1.3 4,315,183 - Psest_4133 0.86 +1.9 4,315,306 + +0.9 4,315,467 + Psest_4134 0.31 -0.2 4,315,467 + Psest_4134 0.31 -0.4 4,315,467 + Psest_4134 0.31 -0.6 4,315,468 - Psest_4134 0.32 +1.5 4,315,468 - Psest_4134 0.32 +0.8 4,315,687 - +0.0 4,315,687 - +2.1 4,315,689 + -1.5 4,315,689 + -0.4 4,315,689 + -0.4 4,315,689 + +0.2 4,315,689 + -1.4 4,315,689 + -0.6 4,315,690 - +1.1 4,315,690 - +1.6 4,315,690 - +1.3 4,315,690 - +1.9 4,315,690 - +0.5 4,315,690 - +1.6 4,315,704 + -0.5 4,315,704 + -1.6 4,315,704 + -0.2 4,315,705 - -0.2 4,315,705 - +1.8 4,315,705 - +2.2 4,315,705 - +1.4 4,315,746 + -1.9 4,315,747 - +1.4 4,315,856 + Psest_4135 0.12 -0.5 4,316,142 + Psest_4135 0.75 -0.8 4,316,142 + Psest_4135 0.75 -1.7 4,316,142 + Psest_4135 0.75 +0.4 4,316,142 + Psest_4135 0.75 -1.6 4,316,142 + Psest_4135 0.75 -0.3 4,316,142 + Psest_4135 0.75 -1.5 4,316,142 + Psest_4135 0.75 +0.0 4,316,142 + Psest_4135 0.75 -0.8 4,316,142 + Psest_4135 0.75 -0.9 4,316,143 - Psest_4135 0.75 +1.0 4,316,143 - Psest_4135 0.75 +2.0 4,316,143 - Psest_4135 0.75 +0.6 4,316,143 - Psest_4135 0.75 +0.3 4,316,299 + -2.9 4,316,299 + +1.0 4,316,299 + -1.8 4,316,299 + -1.0 4,316,299 + -0.1 4,316,300 - -0.3
Or see this region's nucleotide sequence