Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0384 and Psest_0385 are separated by 313 nucleotides Psest_0385 and Psest_0386 are separated by 0 nucleotides Psest_0386 and Psest_0387 overlap by 11 nucleotides
Psest_0384: Psest_0384 - outer membrane porin, OprD family., at 413,248 to 414,519
_0384
Psest_0385: Psest_0385 - Conserved secreted protein, at 414,833 to 415,336
_0385
Psest_0386: Psest_0386 - Membrane protease subunits, stomatin/prohibitin homologs, at 415,337 to 416,095
_0386
Psest_0387: Psest_0387 - Membrane-bound serine protease (ClpP class), at 416,085 to 417,464
_0387
Position (kb) 415
416
417 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 414.380 kb on + strand, within Psest_0384 at 414.380 kb on + strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.520 kb on + strand at 414.690 kb on + strand at 414.691 kb on - strand at 414.719 kb on - strand at 414.719 kb on - strand at 414.771 kb on - strand at 414.847 kb on - strand at 415.128 kb on + strand, within Psest_0385 at 415.129 kb on - strand, within Psest_0385 at 415.129 kb on - strand, within Psest_0385 at 415.328 kb on + strand at 415.328 kb on + strand at 415.328 kb on + strand at 415.328 kb on + strand at 415.328 kb on + strand at 415.328 kb on + strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.443 kb on + strand, within Psest_0386 at 415.443 kb on + strand, within Psest_0386 at 415.444 kb on - strand, within Psest_0386 at 415.782 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.830 kb on + strand, within Psest_0386 at 415.831 kb on - strand, within Psest_0386 at 415.831 kb on - strand, within Psest_0386 at 415.831 kb on - strand, within Psest_0386 at 415.831 kb on - strand, within Psest_0386 at 415.831 kb on - strand, within Psest_0386 at 415.831 kb on - strand, within Psest_0386 at 415.831 kb on - strand, within Psest_0386 at 416.060 kb on + strand at 416.060 kb on + strand at 416.089 kb on - strand at 416.406 kb on + strand, within Psest_0387 at 416.557 kb on + strand, within Psest_0387 at 416.557 kb on + strand, within Psest_0387 at 416.558 kb on - strand, within Psest_0387 at 416.558 kb on - strand, within Psest_0387 at 416.558 kb on - strand, within Psest_0387 at 416.558 kb on - strand, within Psest_0387 at 416.585 kb on - strand, within Psest_0387 at 416.665 kb on + strand, within Psest_0387 at 416.665 kb on + strand, within Psest_0387 at 416.665 kb on + strand, within Psest_0387 at 416.665 kb on + strand, within Psest_0387 at 416.727 kb on + strand, within Psest_0387 at 416.728 kb on - strand, within Psest_0387 at 416.854 kb on + strand, within Psest_0387 at 416.968 kb on + strand, within Psest_0387 at 416.968 kb on + strand, within Psest_0387 at 416.968 kb on + strand, within Psest_0387 at 416.968 kb on + strand, within Psest_0387 at 416.969 kb on - strand, within Psest_0387 at 416.969 kb on - strand, within Psest_0387 at 416.969 kb on - strand, within Psest_0387 at 417.072 kb on - strand, within Psest_0387
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 414,380 + Psest_0384 0.89 -0.1 414,380 + Psest_0384 0.89 +0.1 414,381 - Psest_0384 0.89 -1.1 414,381 - Psest_0384 0.89 -0.9 414,381 - Psest_0384 0.89 -0.0 414,381 - Psest_0384 0.89 +1.9 414,381 - Psest_0384 0.89 +1.0 414,520 + +0.4 414,690 + -0.6 414,691 - -1.6 414,719 - -1.9 414,719 - -1.1 414,771 - -3.0 414,847 - +0.6 415,128 + Psest_0385 0.59 -1.0 415,129 - Psest_0385 0.59 +1.8 415,129 - Psest_0385 0.59 +0.4 415,328 + -1.2 415,328 + +0.9 415,328 + -0.3 415,328 + +0.1 415,328 + -0.1 415,328 + -0.5 415,329 - +0.4 415,329 - -0.2 415,329 - -0.3 415,329 - -0.2 415,329 - +0.6 415,329 - -1.0 415,329 - +0.8 415,329 - +1.0 415,329 - +0.4 415,329 - +1.6 415,329 - +1.6 415,329 - -0.3 415,443 + Psest_0386 0.14 -0.4 415,443 + Psest_0386 0.14 +0.4 415,444 - Psest_0386 0.14 -0.4 415,782 + Psest_0386 0.59 -0.2 415,830 + Psest_0386 0.65 -1.1 415,830 + Psest_0386 0.65 -1.9 415,830 + Psest_0386 0.65 -2.0 415,830 + Psest_0386 0.65 -0.6 415,830 + Psest_0386 0.65 +0.6 415,830 + Psest_0386 0.65 -0.0 415,830 + Psest_0386 0.65 -0.2 415,830 + Psest_0386 0.65 +0.6 415,830 + Psest_0386 0.65 -1.6 415,830 + Psest_0386 0.65 +0.6 415,831 - Psest_0386 0.65 -0.8 415,831 - Psest_0386 0.65 -0.5 415,831 - Psest_0386 0.65 -0.9 415,831 - Psest_0386 0.65 -1.0 415,831 - Psest_0386 0.65 +0.7 415,831 - Psest_0386 0.65 -2.0 415,831 - Psest_0386 0.65 -0.8 416,060 + -1.3 416,060 + +0.3 416,089 - -2.3 416,406 + Psest_0387 0.23 -0.7 416,557 + Psest_0387 0.34 +1.4 416,557 + Psest_0387 0.34 -0.1 416,558 - Psest_0387 0.34 +0.4 416,558 - Psest_0387 0.34 -0.3 416,558 - Psest_0387 0.34 -0.7 416,558 - Psest_0387 0.34 +0.4 416,585 - Psest_0387 0.36 -0.7 416,665 + Psest_0387 0.42 -0.6 416,665 + Psest_0387 0.42 +0.1 416,665 + Psest_0387 0.42 +0.1 416,665 + Psest_0387 0.42 +0.1 416,727 + Psest_0387 0.47 +1.6 416,728 - Psest_0387 0.47 -1.6 416,854 + Psest_0387 0.56 -1.7 416,968 + Psest_0387 0.64 +0.4 416,968 + Psest_0387 0.64 +1.7 416,968 + Psest_0387 0.64 -2.5 416,968 + Psest_0387 0.64 -0.1 416,969 - Psest_0387 0.64 +0.1 416,969 - Psest_0387 0.64 +0.1 416,969 - Psest_0387 0.64 -0.0 417,072 - Psest_0387 0.72 +0.8
Or see this region's nucleotide sequence