Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0382 and Psest_0383 are separated by 2 nucleotides Psest_0383 and Psest_0384 are separated by 108 nucleotides Psest_0384 and Psest_0385 are separated by 313 nucleotides Psest_0385 and Psest_0386 are separated by 0 nucleotides
Psest_0382: Psest_0382 - Uncharacterized iron-regulated membrane protein, at 411,287 to 412,813
_0382
Psest_0383: Psest_0383 - Protein of unknown function (DUF3325)., at 412,816 to 413,139
_0383
Psest_0384: Psest_0384 - outer membrane porin, OprD family., at 413,248 to 414,519
_0384
Psest_0385: Psest_0385 - Conserved secreted protein, at 414,833 to 415,336
_0385
Psest_0386: Psest_0386 - Membrane protease subunits, stomatin/prohibitin homologs, at 415,337 to 416,095
_0386
Position (kb) 413
414
415 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 412.292 kb on + strand, within Psest_0382 at 412.292 kb on + strand, within Psest_0382 at 412.293 kb on - strand, within Psest_0382 at 412.293 kb on - strand, within Psest_0382 at 413.015 kb on + strand, within Psest_0383 at 413.138 kb on - strand at 413.250 kb on - strand at 413.267 kb on + strand at 413.310 kb on - strand at 413.377 kb on - strand, within Psest_0384 at 413.377 kb on - strand, within Psest_0384 at 413.378 kb on + strand, within Psest_0384 at 413.379 kb on - strand, within Psest_0384 at 413.454 kb on - strand, within Psest_0384 at 413.501 kb on + strand, within Psest_0384 at 413.501 kb on + strand, within Psest_0384 at 413.501 kb on + strand, within Psest_0384 at 413.501 kb on + strand, within Psest_0384 at 413.501 kb on + strand, within Psest_0384 at 413.502 kb on - strand, within Psest_0384 at 413.502 kb on - strand, within Psest_0384 at 413.502 kb on - strand, within Psest_0384 at 413.543 kb on + strand, within Psest_0384 at 413.543 kb on + strand, within Psest_0384 at 413.639 kb on + strand, within Psest_0384 at 413.639 kb on + strand, within Psest_0384 at 413.640 kb on - strand, within Psest_0384 at 413.681 kb on + strand, within Psest_0384 at 413.681 kb on + strand, within Psest_0384 at 413.681 kb on + strand, within Psest_0384 at 413.681 kb on + strand, within Psest_0384 at 413.682 kb on - strand, within Psest_0384 at 413.682 kb on - strand, within Psest_0384 at 413.682 kb on - strand, within Psest_0384 at 413.738 kb on + strand, within Psest_0384 at 413.738 kb on + strand, within Psest_0384 at 413.739 kb on - strand, within Psest_0384 at 413.801 kb on + strand, within Psest_0384 at 413.801 kb on + strand, within Psest_0384 at 413.801 kb on + strand, within Psest_0384 at 413.801 kb on + strand, within Psest_0384 at 413.801 kb on + strand, within Psest_0384 at 413.802 kb on - strand, within Psest_0384 at 413.802 kb on - strand, within Psest_0384 at 413.802 kb on - strand, within Psest_0384 at 413.802 kb on - strand, within Psest_0384 at 413.802 kb on - strand, within Psest_0384 at 413.802 kb on - strand, within Psest_0384 at 413.813 kb on + strand, within Psest_0384 at 413.813 kb on + strand, within Psest_0384 at 413.813 kb on + strand, within Psest_0384 at 413.813 kb on + strand, within Psest_0384 at 413.813 kb on + strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.870 kb on + strand, within Psest_0384 at 414.190 kb on + strand, within Psest_0384 at 414.212 kb on + strand, within Psest_0384 at 414.212 kb on + strand, within Psest_0384 at 414.212 kb on + strand, within Psest_0384 at 414.213 kb on - strand, within Psest_0384 at 414.290 kb on + strand, within Psest_0384 at 414.290 kb on + strand, within Psest_0384 at 414.380 kb on + strand, within Psest_0384 at 414.380 kb on + strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.520 kb on + strand at 414.690 kb on + strand at 414.691 kb on - strand at 414.719 kb on - strand at 414.719 kb on - strand at 414.771 kb on - strand at 414.847 kb on - strand at 415.128 kb on + strand, within Psest_0385 at 415.129 kb on - strand, within Psest_0385 at 415.129 kb on - strand, within Psest_0385 at 415.328 kb on + strand at 415.328 kb on + strand at 415.328 kb on + strand at 415.328 kb on + strand at 415.328 kb on + strand at 415.328 kb on + strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.443 kb on + strand, within Psest_0386 at 415.443 kb on + strand, within Psest_0386 at 415.444 kb on - strand, within Psest_0386
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 412,292 + Psest_0382 0.66 +0.6 412,292 + Psest_0382 0.66 +2.4 412,293 - Psest_0382 0.66 +0.9 412,293 - Psest_0382 0.66 -0.9 413,015 + Psest_0383 0.61 +0.8 413,138 - +0.1 413,250 - -0.2 413,267 + -0.8 413,310 - +0.4 413,377 - Psest_0384 0.10 +2.1 413,377 - Psest_0384 0.10 +1.1 413,378 + Psest_0384 0.10 -0.1 413,379 - Psest_0384 0.10 +1.4 413,454 - Psest_0384 0.16 +1.9 413,501 + Psest_0384 0.20 -1.6 413,501 + Psest_0384 0.20 -1.5 413,501 + Psest_0384 0.20 -0.3 413,501 + Psest_0384 0.20 +1.3 413,501 + Psest_0384 0.20 +0.6 413,502 - Psest_0384 0.20 +1.9 413,502 - Psest_0384 0.20 +0.1 413,502 - Psest_0384 0.20 +1.4 413,543 + Psest_0384 0.23 -0.7 413,543 + Psest_0384 0.23 +1.0 413,639 + Psest_0384 0.31 +1.9 413,639 + Psest_0384 0.31 -0.9 413,640 - Psest_0384 0.31 +0.9 413,681 + Psest_0384 0.34 -1.0 413,681 + Psest_0384 0.34 +1.1 413,681 + Psest_0384 0.34 -1.5 413,681 + Psest_0384 0.34 +0.8 413,682 - Psest_0384 0.34 +0.1 413,682 - Psest_0384 0.34 +0.2 413,682 - Psest_0384 0.34 +1.4 413,738 + Psest_0384 0.39 +0.5 413,738 + Psest_0384 0.39 -0.4 413,739 - Psest_0384 0.39 +1.4 413,801 + Psest_0384 0.43 -2.5 413,801 + Psest_0384 0.43 -1.0 413,801 + Psest_0384 0.43 +1.2 413,801 + Psest_0384 0.43 +1.1 413,801 + Psest_0384 0.43 -1.2 413,802 - Psest_0384 0.44 +2.9 413,802 - Psest_0384 0.44 +0.1 413,802 - Psest_0384 0.44 +1.0 413,802 - Psest_0384 0.44 -0.5 413,802 - Psest_0384 0.44 -0.5 413,802 - Psest_0384 0.44 -0.0 413,813 + Psest_0384 0.44 -0.3 413,813 + Psest_0384 0.44 -0.5 413,813 + Psest_0384 0.44 +0.7 413,813 + Psest_0384 0.44 +1.1 413,813 + Psest_0384 0.44 -0.9 413,814 - Psest_0384 0.44 -1.8 413,814 - Psest_0384 0.44 -0.8 413,814 - Psest_0384 0.44 -1.0 413,814 - Psest_0384 0.44 -1.1 413,814 - Psest_0384 0.44 +0.8 413,814 - Psest_0384 0.44 -0.6 413,814 - Psest_0384 0.44 -0.2 413,814 - Psest_0384 0.44 +0.4 413,814 - Psest_0384 0.44 -0.6 413,814 - Psest_0384 0.44 -1.1 413,814 - Psest_0384 0.44 -0.5 413,870 + Psest_0384 0.49 -0.8 414,190 + Psest_0384 0.74 -0.6 414,212 + Psest_0384 0.76 -1.2 414,212 + Psest_0384 0.76 -0.2 414,212 + Psest_0384 0.76 -0.2 414,213 - Psest_0384 0.76 +1.0 414,290 + Psest_0384 0.82 +1.1 414,290 + Psest_0384 0.82 -0.1 414,380 + Psest_0384 0.89 -0.1 414,380 + Psest_0384 0.89 +0.1 414,381 - Psest_0384 0.89 -1.1 414,381 - Psest_0384 0.89 -0.9 414,381 - Psest_0384 0.89 -0.0 414,381 - Psest_0384 0.89 +1.9 414,381 - Psest_0384 0.89 +1.0 414,520 + +0.4 414,690 + -0.6 414,691 - -1.6 414,719 - -1.9 414,719 - -1.1 414,771 - -3.0 414,847 - +0.6 415,128 + Psest_0385 0.59 -1.0 415,129 - Psest_0385 0.59 +1.8 415,129 - Psest_0385 0.59 +0.4 415,328 + -1.2 415,328 + +0.9 415,328 + -0.3 415,328 + +0.1 415,328 + -0.1 415,328 + -0.5 415,329 - +0.4 415,329 - -0.2 415,329 - -0.3 415,329 - -0.2 415,329 - +0.6 415,329 - -1.0 415,329 - +0.8 415,329 - +1.0 415,329 - +0.4 415,329 - +1.6 415,329 - +1.6 415,329 - -0.3 415,443 + Psest_0386 0.14 -0.4 415,443 + Psest_0386 0.14 +0.4 415,444 - Psest_0386 0.14 -0.4
Or see this region's nucleotide sequence