Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3866 and Psest_3867 are separated by 164 nucleotides Psest_3867 and Psest_3868 are separated by 98 nucleotides Psest_3868 and Psest_3869 are separated by 33 nucleotides Psest_3869 and Psest_3870 are separated by 59 nucleotides
Psest_3866: Psest_3866 - threonine ammonia-lyase, biosynthetic, long form, at 4,037,308 to 4,038,822
_3866
Psest_3867: Psest_3867 - ribose 5-phosphate isomerase, at 4,038,987 to 4,039,658
_3867
Psest_3868: Psest_3868 - Uncharacterized conserved protein, at 4,039,757 to 4,040,104
_3868
Psest_3869: Psest_3869 - Uncharacterized protein conserved in bacteria, at 4,040,138 to 4,041,034
_3869
Psest_3870: Psest_3870 - Uncharacterized protein conserved in bacteria, at 4,041,094 to 4,042,014
_3870
Position (kb)
4039
4040
4041 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4038.779 kb on + strand at 4038.809 kb on + strand at 4038.809 kb on + strand at 4038.809 kb on + strand at 4038.810 kb on - strand at 4038.950 kb on - strand at 4038.950 kb on - strand at 4038.950 kb on - strand at 4038.951 kb on + strand at 4039.853 kb on + strand, within Psest_3868 at 4039.854 kb on - strand, within Psest_3868 at 4039.955 kb on + strand, within Psest_3868 at 4039.955 kb on + strand, within Psest_3868 at 4039.955 kb on + strand, within Psest_3868 at 4039.955 kb on + strand, within Psest_3868 at 4039.955 kb on + strand, within Psest_3868 at 4039.956 kb on - strand, within Psest_3868 at 4039.956 kb on - strand, within Psest_3868 at 4039.956 kb on - strand, within Psest_3868 at 4039.956 kb on - strand, within Psest_3868 at 4039.956 kb on - strand, within Psest_3868 at 4040.006 kb on + strand, within Psest_3868 at 4040.006 kb on + strand, within Psest_3868 at 4040.006 kb on + strand, within Psest_3868 at 4040.006 kb on + strand, within Psest_3868 at 4040.006 kb on + strand, within Psest_3868 at 4040.006 kb on + strand, within Psest_3868 at 4040.006 kb on + strand, within Psest_3868 at 4040.006 kb on + strand, within Psest_3868 at 4040.163 kb on + strand at 4040.163 kb on + strand at 4040.163 kb on + strand at 4040.163 kb on + strand at 4040.164 kb on - strand at 4040.164 kb on - strand at 4040.164 kb on - strand at 4040.164 kb on - strand at 4040.164 kb on - strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.190 kb on + strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.191 kb on - strand at 4040.416 kb on + strand, within Psest_3869 at 4040.417 kb on - strand, within Psest_3869 at 4040.598 kb on - strand, within Psest_3869 at 4040.661 kb on + strand, within Psest_3869 at 4040.661 kb on + strand, within Psest_3869 at 4040.661 kb on + strand, within Psest_3869 at 4040.662 kb on - strand, within Psest_3869 at 4040.662 kb on - strand, within Psest_3869 at 4040.679 kb on + strand, within Psest_3869 at 4040.679 kb on + strand, within Psest_3869 at 4040.679 kb on + strand, within Psest_3869 at 4040.679 kb on + strand, within Psest_3869 at 4040.679 kb on + strand, within Psest_3869 at 4040.679 kb on + strand, within Psest_3869 at 4040.680 kb on - strand, within Psest_3869 at 4040.680 kb on - strand, within Psest_3869 at 4040.794 kb on + strand, within Psest_3869 at 4040.795 kb on - strand, within Psest_3869 at 4041.034 kb on + strand at 4041.084 kb on + strand at 4041.084 kb on + strand at 4041.084 kb on + strand at 4041.085 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 4,038,779 + -2.6 4,038,809 + -2.8 4,038,809 + -4.3 4,038,809 + -2.9 4,038,810 - -1.6 4,038,950 - +0.8 4,038,950 - +1.7 4,038,950 - +2.1 4,038,951 + -3.2 4,039,853 + Psest_3868 0.28 -0.8 4,039,854 - Psest_3868 0.28 +1.1 4,039,955 + Psest_3868 0.57 +0.1 4,039,955 + Psest_3868 0.57 +0.8 4,039,955 + Psest_3868 0.57 +0.2 4,039,955 + Psest_3868 0.57 -0.5 4,039,955 + Psest_3868 0.57 +1.1 4,039,956 - Psest_3868 0.57 +1.3 4,039,956 - Psest_3868 0.57 -0.3 4,039,956 - Psest_3868 0.57 -1.6 4,039,956 - Psest_3868 0.57 +0.6 4,039,956 - Psest_3868 0.57 -0.1 4,040,006 + Psest_3868 0.72 +0.6 4,040,006 + Psest_3868 0.72 +1.6 4,040,006 + Psest_3868 0.72 -0.3 4,040,006 + Psest_3868 0.72 +1.8 4,040,006 + Psest_3868 0.72 +0.1 4,040,006 + Psest_3868 0.72 +0.6 4,040,006 + Psest_3868 0.72 +0.5 4,040,006 + Psest_3868 0.72 +0.2 4,040,163 + -0.5 4,040,163 + -0.3 4,040,163 + -1.5 4,040,163 + -1.8 4,040,164 - +1.2 4,040,164 - +1.2 4,040,164 - +1.6 4,040,164 - +1.3 4,040,164 - +0.9 4,040,190 + -0.9 4,040,190 + -0.2 4,040,190 + +1.4 4,040,190 + -1.6 4,040,190 + -0.4 4,040,190 + -1.0 4,040,190 + +0.0 4,040,190 + -1.7 4,040,190 + -0.7 4,040,190 + -2.7 4,040,190 + +0.2 4,040,190 + -0.4 4,040,190 + -0.3 4,040,190 + -2.3 4,040,190 + -1.1 4,040,190 + +1.5 4,040,190 + -2.1 4,040,190 + +0.7 4,040,190 + +0.9 4,040,190 + -1.6 4,040,190 + -0.8 4,040,191 - +1.0 4,040,191 - +1.4 4,040,191 - +1.2 4,040,191 - +1.2 4,040,191 - +1.2 4,040,191 - +1.4 4,040,191 - +1.8 4,040,191 - -0.3 4,040,191 - +0.2 4,040,191 - +0.8 4,040,191 - +0.7 4,040,191 - +0.1 4,040,191 - +1.4 4,040,191 - +0.6 4,040,191 - +0.3 4,040,191 - +0.6 4,040,191 - +0.6 4,040,191 - +1.3 4,040,191 - +0.8 4,040,191 - +1.3 4,040,191 - -0.1 4,040,416 + Psest_3869 0.31 -0.6 4,040,417 - Psest_3869 0.31 +1.1 4,040,598 - Psest_3869 0.51 -0.7 4,040,661 + Psest_3869 0.58 +0.7 4,040,661 + Psest_3869 0.58 -0.2 4,040,661 + Psest_3869 0.58 -0.3 4,040,662 - Psest_3869 0.58 -0.2 4,040,662 - Psest_3869 0.58 -0.3 4,040,679 + Psest_3869 0.60 -0.2 4,040,679 + Psest_3869 0.60 -1.2 4,040,679 + Psest_3869 0.60 -2.4 4,040,679 + Psest_3869 0.60 -1.6 4,040,679 + Psest_3869 0.60 -0.9 4,040,679 + Psest_3869 0.60 +0.0 4,040,680 - Psest_3869 0.60 -0.9 4,040,680 - Psest_3869 0.60 +0.4 4,040,794 + Psest_3869 0.73 -0.7 4,040,795 - Psest_3869 0.73 -1.3 4,041,034 + -0.2 4,041,084 + +0.4 4,041,084 + +0.6 4,041,084 + +1.5 4,041,085 - -0.7
Or see this region's nucleotide sequence