Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3382 and Psest_3383 are separated by 81 nucleotides Psest_3383 and Psest_3384 are separated by 7 nucleotides Psest_3384 and Psest_3385 are separated by 103 nucleotides
Psest_3382: Psest_3382 - ribosomal protein S20, at 3,553,724 to 3,554,002
_3382
Psest_3383: Psest_3383 - Uncharacterized protein conserved in bacteria, at 3,554,084 to 3,554,548
_3383
Psest_3384: Psest_3384 - glutamate 5-kinase, at 3,554,556 to 3,555,674
_3384
Psest_3385: Psest_3385 - Obg family GTPase CgtA, at 3,555,778 to 3,556,995
_3385
Position (kb)
3554
3555
3556 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3553.571 kb on + strand at 3553.572 kb on - strand at 3553.572 kb on - strand at 3554.000 kb on + strand at 3554.164 kb on - strand, within Psest_3383 at 3554.164 kb on - strand, within Psest_3383 at 3554.183 kb on - strand, within Psest_3383 at 3554.228 kb on + strand, within Psest_3383 at 3554.229 kb on - strand, within Psest_3383 at 3554.385 kb on + strand, within Psest_3383 at 3554.385 kb on + strand, within Psest_3383 at 3554.386 kb on - strand, within Psest_3383 at 3554.467 kb on + strand, within Psest_3383 at 3554.533 kb on + strand at 3554.585 kb on + strand at 3554.585 kb on + strand at 3554.586 kb on - strand at 3554.586 kb on - strand at 3554.586 kb on - strand at 3554.586 kb on - strand at 3554.586 kb on - strand at 3554.586 kb on - strand at 3554.586 kb on - strand at 3554.586 kb on - strand at 3554.606 kb on - strand at 3554.606 kb on - strand at 3554.606 kb on - strand at 3554.671 kb on + strand, within Psest_3384 at 3554.671 kb on + strand, within Psest_3384 at 3554.671 kb on + strand, within Psest_3384 at 3554.672 kb on - strand, within Psest_3384 at 3554.672 kb on - strand, within Psest_3384 at 3554.672 kb on - strand, within Psest_3384 at 3554.672 kb on - strand, within Psest_3384 at 3554.672 kb on - strand, within Psest_3384 at 3554.672 kb on - strand, within Psest_3384 at 3554.672 kb on - strand, within Psest_3384 at 3554.672 kb on - strand, within Psest_3384 at 3554.672 kb on - strand, within Psest_3384 at 3554.672 kb on - strand, within Psest_3384 at 3554.672 kb on - strand, within Psest_3384 at 3554.672 kb on - strand, within Psest_3384 at 3554.672 kb on - strand, within Psest_3384 at 3554.672 kb on - strand, within Psest_3384 at 3554.672 kb on - strand, within Psest_3384 at 3554.672 kb on - strand, within Psest_3384 at 3554.672 kb on - strand, within Psest_3384 at 3554.692 kb on - strand, within Psest_3384 at 3554.972 kb on + strand, within Psest_3384 at 3554.973 kb on - strand, within Psest_3384 at 3554.973 kb on - strand, within Psest_3384 at 3554.973 kb on - strand, within Psest_3384 at 3554.973 kb on - strand, within Psest_3384 at 3554.973 kb on - strand, within Psest_3384 at 3554.973 kb on - strand, within Psest_3384 at 3554.973 kb on - strand, within Psest_3384 at 3554.973 kb on - strand, within Psest_3384 at 3555.296 kb on + strand, within Psest_3384 at 3555.297 kb on - strand, within Psest_3384 at 3555.306 kb on - strand, within Psest_3384 at 3555.323 kb on - strand, within Psest_3384 at 3555.687 kb on + strand at 3555.687 kb on + strand at 3555.687 kb on + strand at 3555.688 kb on - strand at 3555.688 kb on - strand at 3555.690 kb on - strand at 3555.788 kb on + strand at 3555.788 kb on + strand at 3555.788 kb on + strand at 3555.788 kb on + strand at 3555.789 kb on - strand at 3555.789 kb on - strand at 3555.789 kb on - strand at 3555.789 kb on - strand at 3555.789 kb on - strand at 3555.789 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 3,553,571 + +1.3 3,553,572 - -1.5 3,553,572 - -2.0 3,554,000 + -1.5 3,554,164 - Psest_3383 0.17 -0.4 3,554,164 - Psest_3383 0.17 -0.5 3,554,183 - Psest_3383 0.21 -0.3 3,554,228 + Psest_3383 0.31 -1.7 3,554,229 - Psest_3383 0.31 -2.6 3,554,385 + Psest_3383 0.65 -1.5 3,554,385 + Psest_3383 0.65 -0.4 3,554,386 - Psest_3383 0.65 +0.2 3,554,467 + Psest_3383 0.82 -1.0 3,554,533 + -1.6 3,554,585 + -2.9 3,554,585 + -3.9 3,554,586 - -0.9 3,554,586 - -1.8 3,554,586 - -1.4 3,554,586 - -3.2 3,554,586 - -1.0 3,554,586 - -0.4 3,554,586 - -2.1 3,554,586 - -3.0 3,554,606 - -3.0 3,554,606 - -2.3 3,554,606 - +0.4 3,554,671 + Psest_3384 0.10 -2.4 3,554,671 + Psest_3384 0.10 -2.7 3,554,671 + Psest_3384 0.10 -2.4 3,554,672 - Psest_3384 0.10 -1.4 3,554,672 - Psest_3384 0.10 -1.5 3,554,672 - Psest_3384 0.10 -1.1 3,554,672 - Psest_3384 0.10 -2.0 3,554,672 - Psest_3384 0.10 -2.1 3,554,672 - Psest_3384 0.10 -1.7 3,554,672 - Psest_3384 0.10 -0.5 3,554,672 - Psest_3384 0.10 -3.2 3,554,672 - Psest_3384 0.10 -1.0 3,554,672 - Psest_3384 0.10 -2.0 3,554,672 - Psest_3384 0.10 -0.9 3,554,672 - Psest_3384 0.10 -2.6 3,554,672 - Psest_3384 0.10 -1.1 3,554,672 - Psest_3384 0.10 -1.8 3,554,672 - Psest_3384 0.10 -3.9 3,554,672 - Psest_3384 0.10 -1.4 3,554,672 - Psest_3384 0.10 -2.6 3,554,692 - Psest_3384 0.12 -1.9 3,554,972 + Psest_3384 0.37 -3.3 3,554,973 - Psest_3384 0.37 -2.7 3,554,973 - Psest_3384 0.37 -2.1 3,554,973 - Psest_3384 0.37 -1.1 3,554,973 - Psest_3384 0.37 -1.2 3,554,973 - Psest_3384 0.37 -3.7 3,554,973 - Psest_3384 0.37 -0.9 3,554,973 - Psest_3384 0.37 -2.0 3,554,973 - Psest_3384 0.37 -2.3 3,555,296 + Psest_3384 0.66 -1.1 3,555,297 - Psest_3384 0.66 -3.3 3,555,306 - Psest_3384 0.67 -1.0 3,555,323 - Psest_3384 0.69 -1.9 3,555,687 + -1.5 3,555,687 + -2.7 3,555,687 + -2.3 3,555,688 - +0.6 3,555,688 - -0.1 3,555,690 - -0.8 3,555,788 + -1.7 3,555,788 + -2.3 3,555,788 + -1.5 3,555,788 + -3.1 3,555,789 - +1.2 3,555,789 - +0.5 3,555,789 - +1.6 3,555,789 - +0.6 3,555,789 - +1.2 3,555,789 - +0.6
Or see this region's nucleotide sequence