Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3070 and Psest_3071 overlap by 4 nucleotides Psest_3071 and Psest_3072 overlap by 1 nucleotides
Psest_3070: Psest_3070 - 3-oxoadipyl-CoA thiolase, at 3,246,176 to 3,247,381
_3070
Psest_3071: Psest_3071 - Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit, at 3,247,378 to 3,248,175
_3071
Psest_3072: Psest_3072 - Acyl CoA:acetate/3-ketoacid CoA transferase, alpha subunit, at 3,248,175 to 3,249,032
_3072
Position (kb)
3247
3248
3249 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3246.561 kb on + strand, within Psest_3070 at 3246.931 kb on + strand, within Psest_3070 at 3246.932 kb on - strand, within Psest_3070 at 3247.006 kb on - strand, within Psest_3070 at 3247.363 kb on + strand at 3247.363 kb on + strand at 3247.364 kb on - strand at 3247.364 kb on - strand at 3247.364 kb on - strand at 3247.367 kb on + strand at 3247.367 kb on + strand at 3247.367 kb on + strand at 3247.368 kb on - strand at 3247.368 kb on - strand at 3247.368 kb on - strand at 3247.368 kb on - strand at 3247.368 kb on - strand at 3247.368 kb on - strand at 3247.368 kb on - strand at 3247.841 kb on + strand, within Psest_3071 at 3247.841 kb on + strand, within Psest_3071 at 3247.842 kb on - strand, within Psest_3071 at 3247.842 kb on - strand, within Psest_3071 at 3247.842 kb on - strand, within Psest_3071 at 3247.842 kb on - strand, within Psest_3071 at 3248.012 kb on + strand, within Psest_3071 at 3248.012 kb on + strand, within Psest_3071 at 3248.013 kb on - strand, within Psest_3071 at 3248.013 kb on - strand, within Psest_3071 at 3248.150 kb on + strand at 3248.150 kb on + strand at 3248.151 kb on - strand at 3248.151 kb on - strand at 3248.151 kb on - strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.242 kb on + strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.243 kb on - strand at 3248.246 kb on + strand at 3248.247 kb on - strand at 3248.324 kb on - strand, within Psest_3072 at 3248.327 kb on - strand, within Psest_3072 at 3248.327 kb on - strand, within Psest_3072 at 3248.338 kb on + strand, within Psest_3072 at 3248.339 kb on - strand, within Psest_3072 at 3248.701 kb on + strand, within Psest_3072 at 3248.701 kb on + strand, within Psest_3072 at 3248.701 kb on + strand, within Psest_3072 at 3248.853 kb on - strand, within Psest_3072 at 3248.863 kb on + strand, within Psest_3072 at 3248.864 kb on - strand, within Psest_3072 at 3248.954 kb on + strand at 3249.059 kb on - strand at 3249.166 kb on + strand at 3249.174 kb on + strand at 3249.174 kb on + strand at 3249.175 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 3,246,561 + Psest_3070 0.32 -0.3 3,246,931 + Psest_3070 0.63 -0.9 3,246,932 - Psest_3070 0.63 -2.3 3,247,006 - Psest_3070 0.69 +0.3 3,247,363 + -1.2 3,247,363 + -0.4 3,247,364 - +0.5 3,247,364 - +1.4 3,247,364 - +2.1 3,247,367 + -0.7 3,247,367 + -1.4 3,247,367 + +0.2 3,247,368 - -1.0 3,247,368 - +0.5 3,247,368 - +0.4 3,247,368 - +0.4 3,247,368 - -0.1 3,247,368 - +0.5 3,247,368 - -0.7 3,247,841 + Psest_3071 0.58 -1.4 3,247,841 + Psest_3071 0.58 +0.6 3,247,842 - Psest_3071 0.58 +0.7 3,247,842 - Psest_3071 0.58 -0.1 3,247,842 - Psest_3071 0.58 -0.4 3,247,842 - Psest_3071 0.58 -0.1 3,248,012 + Psest_3071 0.79 -0.9 3,248,012 + Psest_3071 0.79 -0.7 3,248,013 - Psest_3071 0.80 +0.0 3,248,013 - Psest_3071 0.80 -0.3 3,248,150 + +0.6 3,248,150 + -1.9 3,248,151 - -1.6 3,248,151 - -0.4 3,248,151 - -0.2 3,248,242 + -2.0 3,248,242 + -0.8 3,248,242 + -0.6 3,248,242 + -0.2 3,248,242 + +0.0 3,248,242 + -0.9 3,248,242 + -0.5 3,248,242 + +1.1 3,248,242 + +0.3 3,248,242 + -0.8 3,248,242 + -0.4 3,248,242 + +0.5 3,248,242 + -1.6 3,248,242 + -0.4 3,248,242 + -0.9 3,248,242 + +0.6 3,248,242 + -0.5 3,248,242 + -0.5 3,248,242 + -0.1 3,248,242 + +1.2 3,248,242 + +0.5 3,248,243 - -0.7 3,248,243 - +0.3 3,248,243 - -1.5 3,248,243 - -1.8 3,248,243 - -0.9 3,248,243 - -0.2 3,248,243 - -0.4 3,248,243 - -0.4 3,248,243 - -0.5 3,248,243 - -1.6 3,248,243 - -0.0 3,248,243 - +0.1 3,248,243 - -0.2 3,248,243 - -0.6 3,248,243 - -0.9 3,248,243 - -1.0 3,248,243 - -0.1 3,248,243 - -0.4 3,248,243 - -0.7 3,248,243 - -1.1 3,248,243 - -1.2 3,248,243 - -1.1 3,248,246 + -1.2 3,248,247 - +0.0 3,248,324 - Psest_3072 0.17 -0.9 3,248,327 - Psest_3072 0.18 -0.6 3,248,327 - Psest_3072 0.18 -1.5 3,248,338 + Psest_3072 0.19 +0.8 3,248,339 - Psest_3072 0.19 -1.0 3,248,701 + Psest_3072 0.61 -0.2 3,248,701 + Psest_3072 0.61 -0.1 3,248,701 + Psest_3072 0.61 -1.6 3,248,853 - Psest_3072 0.79 +0.1 3,248,863 + Psest_3072 0.80 -0.5 3,248,864 - Psest_3072 0.80 -0.4 3,248,954 + -1.1 3,249,059 - +0.5 3,249,166 + -0.6 3,249,174 + +0.6 3,249,174 + -0.6 3,249,175 - -0.8
Or see this region's nucleotide sequence