Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2588 and Psest_2589 are separated by 54 nucleotides Psest_2589 and Psest_2590 are separated by 95 nucleotides Psest_2590 and Psest_2591 are separated by 11 nucleotides
Psest_2588: Psest_2588 - aspartate-semialdehyde dehydrogenase, gamma-proteobacterial, at 2,770,047 to 2,771,159
_2588
Psest_2589: Psest_2589 - 3-isopropylmalate dehydrogenase, at 2,771,214 to 2,772,296
_2589
Psest_2590: Psest_2590 - 3-isopropylmalate dehydratase, small subunit, at 2,772,392 to 2,773,039
_2590
Psest_2591: Psest_2591 - 3-isopropylmalate dehydratase, large subunit, at 2,773,051 to 2,774,478
_2591
Position (kb)
2771
2772
2773 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2771.167 kb on - strand at 2771.167 kb on - strand at 2771.167 kb on - strand at 2771.173 kb on - strand at 2771.173 kb on - strand at 2771.173 kb on - strand at 2771.173 kb on - strand at 2771.173 kb on - strand at 2771.173 kb on - strand at 2771.173 kb on - strand at 2771.175 kb on - strand at 2771.175 kb on - strand at 2771.175 kb on - strand at 2771.467 kb on + strand, within Psest_2589 at 2771.468 kb on - strand, within Psest_2589 at 2771.468 kb on - strand, within Psest_2589 at 2771.632 kb on + strand, within Psest_2589 at 2771.632 kb on + strand, within Psest_2589 at 2771.632 kb on + strand, within Psest_2589 at 2771.632 kb on + strand, within Psest_2589 at 2771.632 kb on + strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.663 kb on - strand, within Psest_2589 at 2771.663 kb on - strand, within Psest_2589 at 2771.663 kb on - strand, within Psest_2589 at 2771.663 kb on - strand, within Psest_2589 at 2771.663 kb on - strand, within Psest_2589 at 2771.822 kb on - strand, within Psest_2589 at 2771.875 kb on + strand, within Psest_2589 at 2771.876 kb on - strand, within Psest_2589 at 2771.876 kb on - strand, within Psest_2589 at 2772.146 kb on - strand, within Psest_2589 at 2772.390 kb on + strand at 2772.391 kb on - strand at 2772.691 kb on - strand, within Psest_2590 at 2773.072 kb on + strand at 2773.072 kb on + strand at 2773.072 kb on + strand at 2773.073 kb on - strand at 2773.073 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 2,771,167 - -0.9 2,771,167 - +2.2 2,771,167 - +2.2 2,771,173 - -0.4 2,771,173 - +0.6 2,771,173 - +0.5 2,771,173 - -0.7 2,771,173 - +0.7 2,771,173 - +0.2 2,771,173 - +0.6 2,771,175 - -0.8 2,771,175 - -0.6 2,771,175 - -1.0 2,771,467 + Psest_2589 0.23 -0.7 2,771,468 - Psest_2589 0.23 -2.5 2,771,468 - Psest_2589 0.23 -0.6 2,771,632 + Psest_2589 0.39 -0.4 2,771,632 + Psest_2589 0.39 -1.2 2,771,632 + Psest_2589 0.39 +2.0 2,771,632 + Psest_2589 0.39 -1.6 2,771,632 + Psest_2589 0.39 -1.2 2,771,633 - Psest_2589 0.39 -1.6 2,771,633 - Psest_2589 0.39 -0.1 2,771,633 - Psest_2589 0.39 -0.4 2,771,633 - Psest_2589 0.39 -2.7 2,771,633 - Psest_2589 0.39 -1.7 2,771,633 - Psest_2589 0.39 -0.4 2,771,633 - Psest_2589 0.39 -0.1 2,771,633 - Psest_2589 0.39 -0.9 2,771,633 - Psest_2589 0.39 -0.8 2,771,633 - Psest_2589 0.39 -1.8 2,771,633 - Psest_2589 0.39 -0.4 2,771,633 - Psest_2589 0.39 -1.3 2,771,633 - Psest_2589 0.39 -2.5 2,771,633 - Psest_2589 0.39 +0.8 2,771,633 - Psest_2589 0.39 -3.2 2,771,633 - Psest_2589 0.39 -1.6 2,771,633 - Psest_2589 0.39 -2.4 2,771,633 - Psest_2589 0.39 +0.2 2,771,633 - Psest_2589 0.39 -1.0 2,771,633 - Psest_2589 0.39 -1.3 2,771,633 - Psest_2589 0.39 -0.5 2,771,633 - Psest_2589 0.39 -2.1 2,771,633 - Psest_2589 0.39 -2.7 2,771,633 - Psest_2589 0.39 -1.8 2,771,633 - Psest_2589 0.39 -0.5 2,771,633 - Psest_2589 0.39 -2.4 2,771,633 - Psest_2589 0.39 -1.0 2,771,633 - Psest_2589 0.39 -2.0 2,771,633 - Psest_2589 0.39 +1.2 2,771,633 - Psest_2589 0.39 +0.9 2,771,663 - Psest_2589 0.41 -1.6 2,771,663 - Psest_2589 0.41 -1.3 2,771,663 - Psest_2589 0.41 +0.5 2,771,663 - Psest_2589 0.41 -1.5 2,771,663 - Psest_2589 0.41 -1.6 2,771,822 - Psest_2589 0.56 -1.4 2,771,875 + Psest_2589 0.61 -2.5 2,771,876 - Psest_2589 0.61 -0.0 2,771,876 - Psest_2589 0.61 -0.9 2,772,146 - Psest_2589 0.86 +0.6 2,772,390 + -3.2 2,772,391 - +2.2 2,772,691 - Psest_2590 0.46 -1.4 2,773,072 + -2.0 2,773,072 + -2.5 2,773,072 + -3.7 2,773,073 - -0.2 2,773,073 - -0.1
Or see this region's nucleotide sequence