Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0214 and Psest_0215 are separated by 130 nucleotides Psest_0215 and Psest_0216 are separated by 0 nucleotides
Psest_0214: Psest_0214 - exodeoxyribonuclease V, alpha subunit, at 220,244 to 222,091
_0214
Psest_0215: Psest_0215 - hypothetical protein, at 222,222 to 222,665
_0215
Psest_0216: Psest_0216 - Predicted permeases, at 222,666 to 223,574
_0216
Position (kb) 222
223 Strain fitness (log2 ratio) -2
-1
0
1
2 at 221.888 kb on + strand, within Psest_0214 at 222.244 kb on + strand at 222.245 kb on - strand at 222.287 kb on + strand, within Psest_0215 at 222.342 kb on + strand, within Psest_0215 at 222.342 kb on + strand, within Psest_0215 at 222.343 kb on - strand, within Psest_0215 at 222.343 kb on - strand, within Psest_0215 at 222.343 kb on - strand, within Psest_0215 at 222.345 kb on + strand, within Psest_0215 at 222.345 kb on + strand, within Psest_0215 at 222.345 kb on + strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.346 kb on - strand, within Psest_0215 at 222.382 kb on - strand, within Psest_0215 at 222.484 kb on + strand, within Psest_0215 at 222.501 kb on + strand, within Psest_0215 at 222.781 kb on + strand, within Psest_0216 at 222.781 kb on + strand at 222.781 kb on + strand, within Psest_0216 at 222.782 kb on - strand, within Psest_0216 at 222.937 kb on - strand, within Psest_0216 at 223.255 kb on + strand, within Psest_0216 at 223.255 kb on + strand, within Psest_0216 at 223.255 kb on + strand, within Psest_0216 at 223.255 kb on + strand, within Psest_0216 at 223.256 kb on - strand, within Psest_0216 at 223.256 kb on - strand, within Psest_0216 at 223.424 kb on + strand, within Psest_0216 at 223.457 kb on - strand, within Psest_0216 at 223.457 kb on - strand, within Psest_0216 at 223.457 kb on - strand, within Psest_0216 at 223.457 kb on - strand, within Psest_0216 at 223.457 kb on - strand, within Psest_0216 at 223.519 kb on + strand at 223.588 kb on + strand at 223.589 kb on - strand at 223.589 kb on - strand at 223.589 kb on - strand at 223.656 kb on + strand at 223.656 kb on + strand at 223.657 kb on - strand at 223.657 kb on - strand at 223.657 kb on - strand at 223.657 kb on - strand at 223.657 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 221,888 + Psest_0214 0.89 +1.8 222,244 + -0.7 222,245 - +0.3 222,287 + Psest_0215 0.15 -0.1 222,342 + Psest_0215 0.27 +1.5 222,342 + Psest_0215 0.27 +0.8 222,343 - Psest_0215 0.27 +0.0 222,343 - Psest_0215 0.27 -0.6 222,343 - Psest_0215 0.27 +1.8 222,345 + Psest_0215 0.28 +0.0 222,345 + Psest_0215 0.28 -0.5 222,345 + Psest_0215 0.28 +0.0 222,346 - Psest_0215 0.28 +0.2 222,346 - Psest_0215 0.28 -1.4 222,346 - Psest_0215 0.28 -1.5 222,346 - Psest_0215 0.28 +0.0 222,346 - Psest_0215 0.28 +0.5 222,346 - Psest_0215 0.28 +0.1 222,346 - Psest_0215 0.28 +0.8 222,346 - Psest_0215 0.28 -0.2 222,346 - Psest_0215 0.28 -0.8 222,382 - Psest_0215 0.36 -0.6 222,484 + Psest_0215 0.59 -1.4 222,501 + Psest_0215 0.63 -1.0 222,781 + Psest_0216 0.13 -0.4 222,781 + +1.8 222,781 + Psest_0216 0.13 +1.2 222,782 - Psest_0216 0.13 -0.2 222,937 - Psest_0216 0.30 -1.1 223,255 + Psest_0216 0.65 -2.4 223,255 + Psest_0216 0.65 -1.4 223,255 + Psest_0216 0.65 -0.8 223,255 + Psest_0216 0.65 -2.6 223,256 - Psest_0216 0.65 -1.0 223,256 - Psest_0216 0.65 +0.8 223,424 + Psest_0216 0.83 +0.5 223,457 - Psest_0216 0.87 +0.1 223,457 - Psest_0216 0.87 -1.3 223,457 - Psest_0216 0.87 -2.5 223,457 - Psest_0216 0.87 +0.6 223,457 - Psest_0216 0.87 -0.5 223,519 + -1.5 223,588 + +0.2 223,589 - -1.4 223,589 - +0.0 223,589 - +1.4 223,656 + -0.8 223,656 + -0.5 223,657 - -0.2 223,657 - +0.2 223,657 - +1.5 223,657 - +1.6 223,657 - -0.5
Or see this region's nucleotide sequence