Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2015 and Psest_2016 overlap by 4 nucleotides Psest_2016 and Psest_2017 are separated by 6 nucleotides Psest_2017 and Psest_2018 overlap by 8 nucleotides
Psest_2015: Psest_2015 - Type II secretory pathway, component PulD, at 2,134,961 to 2,135,770
_2015
Psest_2016: Psest_2016 - hypothetical protein, at 2,135,767 to 2,136,396
_2016
Psest_2017: Psest_2017 - Predicted acyltransferase, at 2,136,403 to 2,136,828
_2017
Psest_2018: Psest_2018 - Uncharacterized conserved protein, at 2,136,821 to 2,137,987
_2018
Position (kb)
2136
2137 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2135.616 kb on + strand, within Psest_2015 at 2135.646 kb on - strand, within Psest_2015 at 2135.717 kb on + strand at 2135.717 kb on + strand at 2135.718 kb on - strand at 2135.758 kb on + strand at 2135.841 kb on + strand, within Psest_2016 at 2135.842 kb on - strand, within Psest_2016 at 2135.864 kb on + strand, within Psest_2016 at 2135.865 kb on - strand, within Psest_2016 at 2135.865 kb on - strand, within Psest_2016 at 2135.865 kb on - strand, within Psest_2016 at 2135.865 kb on - strand, within Psest_2016 at 2135.865 kb on - strand, within Psest_2016 at 2135.873 kb on + strand, within Psest_2016 at 2135.908 kb on + strand, within Psest_2016 at 2135.934 kb on - strand, within Psest_2016 at 2135.976 kb on + strand, within Psest_2016 at 2136.005 kb on - strand, within Psest_2016 at 2136.005 kb on - strand, within Psest_2016 at 2136.128 kb on + strand, within Psest_2016 at 2136.128 kb on + strand, within Psest_2016 at 2136.489 kb on - strand, within Psest_2017 at 2136.489 kb on - strand, within Psest_2017 at 2136.489 kb on - strand, within Psest_2017 at 2136.533 kb on - strand, within Psest_2017 at 2136.776 kb on - strand, within Psest_2017 at 2136.776 kb on - strand, within Psest_2017 at 2136.776 kb on - strand, within Psest_2017 at 2136.822 kb on + strand at 2137.186 kb on + strand, within Psest_2018 at 2137.186 kb on + strand, within Psest_2018 at 2137.187 kb on - strand, within Psest_2018 at 2137.365 kb on + strand, within Psest_2018 at 2137.575 kb on + strand, within Psest_2018 at 2137.575 kb on + strand, within Psest_2018 at 2137.576 kb on - strand, within Psest_2018 at 2137.617 kb on + strand, within Psest_2018 at 2137.618 kb on - strand, within Psest_2018
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 2,135,616 + Psest_2015 0.81 +0.4 2,135,646 - Psest_2015 0.85 -1.5 2,135,717 + -0.3 2,135,717 + +0.2 2,135,718 - +0.6 2,135,758 + -1.1 2,135,841 + Psest_2016 0.12 -0.2 2,135,842 - Psest_2016 0.12 +1.3 2,135,864 + Psest_2016 0.15 -0.8 2,135,865 - Psest_2016 0.16 -1.2 2,135,865 - Psest_2016 0.16 +0.6 2,135,865 - Psest_2016 0.16 -0.4 2,135,865 - Psest_2016 0.16 -2.3 2,135,865 - Psest_2016 0.16 +0.4 2,135,873 + Psest_2016 0.17 -1.5 2,135,908 + Psest_2016 0.22 -1.7 2,135,934 - Psest_2016 0.27 -1.0 2,135,976 + Psest_2016 0.33 +1.1 2,136,005 - Psest_2016 0.38 -0.5 2,136,005 - Psest_2016 0.38 -0.9 2,136,128 + Psest_2016 0.57 -0.6 2,136,128 + Psest_2016 0.57 -0.9 2,136,489 - Psest_2017 0.20 +2.6 2,136,489 - Psest_2017 0.20 +2.1 2,136,489 - Psest_2017 0.20 +0.4 2,136,533 - Psest_2017 0.31 +1.1 2,136,776 - Psest_2017 0.88 +0.6 2,136,776 - Psest_2017 0.88 +0.6 2,136,776 - Psest_2017 0.88 +1.4 2,136,822 + +1.3 2,137,186 + Psest_2018 0.31 -1.9 2,137,186 + Psest_2018 0.31 +0.5 2,137,187 - Psest_2018 0.31 +0.9 2,137,365 + Psest_2018 0.47 +1.3 2,137,575 + Psest_2018 0.65 +0.1 2,137,575 + Psest_2018 0.65 -0.1 2,137,576 - Psest_2018 0.65 +0.3 2,137,617 + Psest_2018 0.68 -0.4 2,137,618 - Psest_2018 0.68 -0.1
Or see this region's nucleotide sequence