Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1979 and Psest_1980 are separated by 8 nucleotides Psest_1980 and Psest_1981 are separated by 339 nucleotides Psest_1981 and Psest_1982 are separated by 134 nucleotides
Psest_1979: Psest_1979 - Nucleotidyltransferase/DNA polymerase involved in DNA repair, at 2,092,694 to 2,094,106
_1979
Psest_1980: Psest_1980 - Uncharacterized conserved protein, at 2,094,115 to 2,094,732
_1980
Psest_1981: Psest_1981 - S-methyl-5-thioribose-1-phosphate isomerase, at 2,095,072 to 2,096,148
_1981
Psest_1982: Psest_1982 - DNA gyrase, A subunit, at 2,096,283 to 2,099,093
_1982
Position (kb)
2095
2096
2097 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2094.117 kb on - strand at 2094.117 kb on - strand at 2094.293 kb on - strand, within Psest_1980 at 2094.344 kb on - strand, within Psest_1980 at 2094.544 kb on + strand, within Psest_1980 at 2094.545 kb on - strand, within Psest_1980 at 2094.545 kb on - strand, within Psest_1980 at 2094.545 kb on - strand, within Psest_1980 at 2094.749 kb on + strand at 2094.759 kb on + strand at 2094.760 kb on - strand at 2094.760 kb on - strand at 2094.895 kb on - strand at 2094.933 kb on + strand at 2094.978 kb on + strand at 2095.104 kb on + strand at 2095.104 kb on + strand at 2095.105 kb on - strand at 2095.183 kb on + strand, within Psest_1981 at 2095.221 kb on + strand, within Psest_1981 at 2095.657 kb on + strand, within Psest_1981 at 2095.657 kb on + strand, within Psest_1981 at 2095.657 kb on + strand, within Psest_1981 at 2095.657 kb on + strand, within Psest_1981 at 2095.657 kb on + strand, within Psest_1981 at 2095.657 kb on + strand, within Psest_1981 at 2095.657 kb on + strand, within Psest_1981 at 2095.657 kb on + strand, within Psest_1981 at 2095.658 kb on - strand, within Psest_1981 at 2095.658 kb on - strand, within Psest_1981 at 2095.658 kb on - strand, within Psest_1981 at 2095.658 kb on - strand, within Psest_1981 at 2095.852 kb on + strand, within Psest_1981 at 2095.852 kb on + strand, within Psest_1981 at 2096.212 kb on + strand at 2096.214 kb on + strand at 2096.264 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 2,094,117 - +1.6 2,094,117 - +0.3 2,094,293 - Psest_1980 0.29 +0.2 2,094,344 - Psest_1980 0.37 +1.0 2,094,544 + Psest_1980 0.69 +0.1 2,094,545 - Psest_1980 0.70 +1.5 2,094,545 - Psest_1980 0.70 +1.6 2,094,545 - Psest_1980 0.70 -0.6 2,094,749 + +0.1 2,094,759 + +0.4 2,094,760 - +1.3 2,094,760 - +0.4 2,094,895 - +0.7 2,094,933 + +1.4 2,094,978 + +1.5 2,095,104 + +0.4 2,095,104 + -0.4 2,095,105 - -0.4 2,095,183 + Psest_1981 0.10 +0.7 2,095,221 + Psest_1981 0.14 +0.7 2,095,657 + Psest_1981 0.54 -0.5 2,095,657 + Psest_1981 0.54 +1.8 2,095,657 + Psest_1981 0.54 -2.5 2,095,657 + Psest_1981 0.54 -0.1 2,095,657 + Psest_1981 0.54 -0.0 2,095,657 + Psest_1981 0.54 +1.6 2,095,657 + Psest_1981 0.54 +0.0 2,095,657 + Psest_1981 0.54 +1.1 2,095,658 - Psest_1981 0.54 -0.0 2,095,658 - Psest_1981 0.54 -0.1 2,095,658 - Psest_1981 0.54 -1.4 2,095,658 - Psest_1981 0.54 -0.6 2,095,852 + Psest_1981 0.72 +1.4 2,095,852 + Psest_1981 0.72 +1.1 2,096,212 + -0.4 2,096,214 + -1.9 2,096,264 + -0.4
Or see this region's nucleotide sequence