Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0189 and Psest_0190 are separated by 2 nucleotides Psest_0190 and Psest_0191 are separated by 21 nucleotides Psest_0191 and Psest_0192 overlap by 4 nucleotides
Psest_0189: Psest_0189 - Negative regulator of beta-lactamase expression, at 185,255 to 186,043
_0189
Psest_0190: Psest_0190 - Uncharacterized conserved protein, at 186,046 to 186,450
_0190
Psest_0191: Psest_0191 - PAS domain S-box, at 186,472 to 188,259
_0191
Psest_0192: Psest_0192 - Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains, at 188,256 to 189,602
_0192
Position (kb) 186
187
188
189 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 185.864 kb on + strand, within Psest_0189 at 185.961 kb on - strand, within Psest_0189 at 186.051 kb on - strand at 186.293 kb on + strand, within Psest_0190 at 186.293 kb on + strand, within Psest_0190 at 186.354 kb on + strand, within Psest_0190 at 186.356 kb on + strand, within Psest_0190 at 186.356 kb on + strand, within Psest_0190 at 186.356 kb on + strand, within Psest_0190 at 186.491 kb on + strand at 186.491 kb on + strand at 186.491 kb on + strand at 186.491 kb on + strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.565 kb on + strand at 186.645 kb on - strand at 186.735 kb on + strand, within Psest_0191 at 187.799 kb on + strand, within Psest_0191 at 188.089 kb on + strand at 188.089 kb on + strand at 188.089 kb on + strand at 188.089 kb on + strand at 188.089 kb on + strand at 188.089 kb on + strand at 188.090 kb on - strand at 188.259 kb on + strand at 188.260 kb on - strand at 188.260 kb on - strand at 188.260 kb on - strand at 188.530 kb on + strand, within Psest_0192 at 188.530 kb on + strand, within Psest_0192 at 188.530 kb on + strand, within Psest_0192 at 188.530 kb on + strand, within Psest_0192 at 188.531 kb on - strand, within Psest_0192 at 188.531 kb on - strand, within Psest_0192 at 188.531 kb on - strand, within Psest_0192 at 188.686 kb on + strand, within Psest_0192 at 188.687 kb on - strand, within Psest_0192 at 188.800 kb on + strand, within Psest_0192 at 188.982 kb on + strand, within Psest_0192
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 185,864 + Psest_0189 0.77 +1.2 185,961 - Psest_0189 0.89 +0.1 186,051 - -0.7 186,293 + Psest_0190 0.61 +0.2 186,293 + Psest_0190 0.61 +0.6 186,354 + Psest_0190 0.76 +1.1 186,356 + Psest_0190 0.77 +0.2 186,356 + Psest_0190 0.77 -0.6 186,356 + Psest_0190 0.77 +0.1 186,491 + +1.4 186,491 + +1.7 186,491 + -0.0 186,491 + +1.7 186,492 - +0.1 186,492 - -0.3 186,492 - +2.2 186,492 - +1.1 186,492 - +0.5 186,492 - -0.2 186,492 - +1.2 186,492 - +0.3 186,492 - -1.0 186,565 + +1.9 186,645 - -1.2 186,735 + Psest_0191 0.15 +1.5 187,799 + Psest_0191 0.74 +0.7 188,089 + +1.8 188,089 + +1.9 188,089 + +3.9 188,089 + +2.5 188,089 + +1.6 188,089 + +1.8 188,090 - +1.3 188,259 + +1.4 188,260 - +0.7 188,260 - +1.2 188,260 - -0.2 188,530 + Psest_0192 0.20 +1.4 188,530 + Psest_0192 0.20 +1.9 188,530 + Psest_0192 0.20 +1.7 188,530 + Psest_0192 0.20 +1.0 188,531 - Psest_0192 0.20 +2.1 188,531 - Psest_0192 0.20 +0.4 188,531 - Psest_0192 0.20 +1.9 188,686 + Psest_0192 0.32 +1.5 188,687 - Psest_0192 0.32 +2.1 188,800 + Psest_0192 0.40 +1.4 188,982 + Psest_0192 0.54 +0.6
Or see this region's nucleotide sequence