Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0188 and Psest_0189 are separated by 112 nucleotides Psest_0189 and Psest_0190 are separated by 2 nucleotides Psest_0190 and Psest_0191 are separated by 21 nucleotides
Psest_0188: Psest_0188 - diguanylate cyclase (GGDEF) domain, at 183,244 to 185,142
_0188
Psest_0189: Psest_0189 - Negative regulator of beta-lactamase expression, at 185,255 to 186,043
_0189
Psest_0190: Psest_0190 - Uncharacterized conserved protein, at 186,046 to 186,450
_0190
Psest_0191: Psest_0191 - PAS domain S-box, at 186,472 to 188,259
_0191
Position (kb) 185
186
187 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 184.306 kb on + strand, within Psest_0188 at 184.307 kb on - strand, within Psest_0188 at 184.524 kb on + strand, within Psest_0188 at 184.529 kb on - strand, within Psest_0188 at 184.529 kb on - strand, within Psest_0188 at 185.178 kb on - strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.332 kb on + strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.333 kb on - strand at 185.378 kb on + strand, within Psest_0189 at 185.378 kb on + strand, within Psest_0189 at 185.378 kb on + strand, within Psest_0189 at 185.378 kb on + strand, within Psest_0189 at 185.378 kb on + strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.379 kb on - strand, within Psest_0189 at 185.442 kb on - strand, within Psest_0189 at 185.442 kb on - strand, within Psest_0189 at 185.864 kb on + strand, within Psest_0189 at 185.961 kb on - strand, within Psest_0189 at 186.051 kb on - strand at 186.293 kb on + strand, within Psest_0190 at 186.293 kb on + strand, within Psest_0190 at 186.354 kb on + strand, within Psest_0190 at 186.356 kb on + strand, within Psest_0190 at 186.356 kb on + strand, within Psest_0190 at 186.356 kb on + strand, within Psest_0190 at 186.491 kb on + strand at 186.491 kb on + strand at 186.491 kb on + strand at 186.491 kb on + strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.492 kb on - strand at 186.565 kb on + strand at 186.645 kb on - strand at 186.735 kb on + strand, within Psest_0191
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 184,306 + Psest_0188 0.56 +1.4 184,307 - Psest_0188 0.56 -0.2 184,524 + Psest_0188 0.67 +1.6 184,529 - Psest_0188 0.68 -1.5 184,529 - Psest_0188 0.68 +0.8 185,178 - -0.6 185,332 + -0.8 185,332 + -1.7 185,332 + -2.0 185,332 + -1.0 185,332 + +0.2 185,332 + -0.4 185,333 - -1.6 185,333 - -1.3 185,333 - +0.2 185,333 - +0.2 185,333 - +0.8 185,333 - -2.3 185,378 + Psest_0189 0.16 -2.0 185,378 + Psest_0189 0.16 -1.7 185,378 + Psest_0189 0.16 -2.2 185,378 + Psest_0189 0.16 -0.3 185,378 + Psest_0189 0.16 -2.3 185,379 - Psest_0189 0.16 -1.0 185,379 - Psest_0189 0.16 -1.7 185,379 - Psest_0189 0.16 -4.1 185,379 - Psest_0189 0.16 -1.8 185,379 - Psest_0189 0.16 -1.1 185,379 - Psest_0189 0.16 -2.0 185,379 - Psest_0189 0.16 -3.8 185,379 - Psest_0189 0.16 -0.7 185,379 - Psest_0189 0.16 -1.3 185,442 - Psest_0189 0.24 +0.5 185,442 - Psest_0189 0.24 -1.9 185,864 + Psest_0189 0.77 +1.2 185,961 - Psest_0189 0.89 +0.1 186,051 - -0.7 186,293 + Psest_0190 0.61 +0.2 186,293 + Psest_0190 0.61 +0.6 186,354 + Psest_0190 0.76 +1.1 186,356 + Psest_0190 0.77 +0.2 186,356 + Psest_0190 0.77 -0.6 186,356 + Psest_0190 0.77 +0.1 186,491 + +1.4 186,491 + +1.7 186,491 + -0.0 186,491 + +1.7 186,492 - +0.1 186,492 - -0.3 186,492 - +2.2 186,492 - +1.1 186,492 - +0.5 186,492 - -0.2 186,492 - +1.2 186,492 - +0.3 186,492 - -1.0 186,565 + +1.9 186,645 - -1.2 186,735 + Psest_0191 0.15 +1.5
Or see this region's nucleotide sequence