Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1857 and Psest_1858 are separated by 216 nucleotides Psest_1858 and Psest_1859 are separated by 13 nucleotides Psest_1859 and Psest_1860 are separated by 87 nucleotides Psest_1860 and Psest_1861 are separated by 34 nucleotides
Psest_1857: Psest_1857 - Isocitrate dehydrogenase kinase/phosphatase, at 1,961,487 to 1,963,208
_1857
Psest_1858: Psest_1858 - hypothetical protein, at 1,963,425 to 1,963,550
_1858
Psest_1859: Psest_1859 - Glutathione S-transferase, at 1,963,564 to 1,964,235
_1859
Psest_1860: Psest_1860 - Uncharacterized conserved protein, at 1,964,323 to 1,964,910
_1860
Psest_1861: Psest_1861 - type I secretion membrane fusion protein, HlyD family, at 1,964,945 to 1,966,297
_1861
Position (kb)
1963
1964
1965 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1962.564 kb on + strand, within Psest_1857 at 1962.564 kb on + strand, within Psest_1857 at 1962.565 kb on - strand, within Psest_1857 at 1962.565 kb on - strand, within Psest_1857 at 1962.644 kb on + strand, within Psest_1857 at 1962.644 kb on + strand, within Psest_1857 at 1962.718 kb on + strand, within Psest_1857 at 1962.718 kb on + strand, within Psest_1857 at 1962.719 kb on - strand, within Psest_1857 at 1962.768 kb on + strand, within Psest_1857 at 1962.816 kb on + strand, within Psest_1857 at 1962.817 kb on - strand, within Psest_1857 at 1962.852 kb on - strand, within Psest_1857 at 1962.915 kb on + strand, within Psest_1857 at 1962.915 kb on + strand, within Psest_1857 at 1962.915 kb on + strand, within Psest_1857 at 1962.936 kb on + strand, within Psest_1857 at 1962.936 kb on + strand, within Psest_1857 at 1962.936 kb on + strand, within Psest_1857 at 1962.936 kb on + strand, within Psest_1857 at 1962.937 kb on - strand, within Psest_1857 at 1963.087 kb on + strand at 1963.113 kb on + strand at 1963.113 kb on + strand at 1963.114 kb on - strand at 1963.114 kb on - strand at 1963.203 kb on - strand at 1963.284 kb on + strand at 1963.284 kb on + strand at 1963.285 kb on - strand at 1963.285 kb on - strand at 1963.285 kb on - strand at 1963.309 kb on - strand at 1963.362 kb on + strand at 1963.362 kb on + strand at 1963.363 kb on - strand at 1963.363 kb on - strand at 1963.363 kb on - strand at 1963.395 kb on + strand at 1963.396 kb on - strand at 1963.403 kb on + strand at 1963.608 kb on + strand at 1963.610 kb on + strand at 1963.610 kb on + strand at 1963.611 kb on - strand at 1963.627 kb on + strand at 1963.627 kb on + strand at 1963.628 kb on - strand at 1963.628 kb on - strand at 1963.628 kb on - strand at 1963.629 kb on + strand at 1963.714 kb on + strand, within Psest_1859 at 1963.714 kb on + strand, within Psest_1859 at 1963.714 kb on + strand, within Psest_1859 at 1963.714 kb on + strand, within Psest_1859 at 1963.715 kb on - strand, within Psest_1859 at 1963.715 kb on - strand, within Psest_1859 at 1963.734 kb on + strand, within Psest_1859 at 1963.734 kb on + strand, within Psest_1859 at 1963.778 kb on + strand, within Psest_1859 at 1963.779 kb on - strand, within Psest_1859 at 1963.779 kb on - strand, within Psest_1859 at 1963.779 kb on - strand, within Psest_1859 at 1963.795 kb on + strand, within Psest_1859 at 1963.795 kb on + strand, within Psest_1859 at 1963.937 kb on + strand, within Psest_1859 at 1964.159 kb on + strand, within Psest_1859 at 1964.159 kb on + strand, within Psest_1859 at 1964.160 kb on - strand, within Psest_1859 at 1964.160 kb on - strand, within Psest_1859 at 1964.160 kb on - strand, within Psest_1859 at 1964.250 kb on - strand at 1964.268 kb on + strand at 1964.545 kb on + strand, within Psest_1860 at 1964.545 kb on + strand, within Psest_1860 at 1964.546 kb on - strand, within Psest_1860 at 1964.664 kb on + strand, within Psest_1860 at 1964.881 kb on + strand at 1964.946 kb on + strand at 1964.947 kb on - strand at 1964.997 kb on + strand at 1964.997 kb on + strand at 1964.997 kb on + strand at 1964.997 kb on + strand at 1964.998 kb on - strand at 1964.998 kb on - strand at 1964.998 kb on - strand at 1964.998 kb on - strand at 1965.118 kb on - strand, within Psest_1861 at 1965.124 kb on + strand, within Psest_1861 at 1965.124 kb on + strand, within Psest_1861 at 1965.124 kb on + strand, within Psest_1861 at 1965.125 kb on - strand, within Psest_1861 at 1965.198 kb on + strand, within Psest_1861 at 1965.198 kb on + strand, within Psest_1861 at 1965.198 kb on + strand, within Psest_1861 at 1965.199 kb on - strand, within Psest_1861
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 1,962,564 + Psest_1857 0.63 -1.5 1,962,564 + Psest_1857 0.63 -0.2 1,962,565 - Psest_1857 0.63 -2.6 1,962,565 - Psest_1857 0.63 -1.4 1,962,644 + Psest_1857 0.67 -0.3 1,962,644 + Psest_1857 0.67 +1.6 1,962,718 + Psest_1857 0.71 -0.2 1,962,718 + Psest_1857 0.71 +0.5 1,962,719 - Psest_1857 0.72 -1.1 1,962,768 + Psest_1857 0.74 -0.4 1,962,816 + Psest_1857 0.77 -1.1 1,962,817 - Psest_1857 0.77 -2.0 1,962,852 - Psest_1857 0.79 -0.8 1,962,915 + Psest_1857 0.83 +0.6 1,962,915 + Psest_1857 0.83 -0.8 1,962,915 + Psest_1857 0.83 +0.8 1,962,936 + Psest_1857 0.84 -0.1 1,962,936 + Psest_1857 0.84 -0.9 1,962,936 + Psest_1857 0.84 +2.8 1,962,936 + Psest_1857 0.84 +0.6 1,962,937 - Psest_1857 0.84 -0.7 1,963,087 + +0.4 1,963,113 + +1.5 1,963,113 + +1.4 1,963,114 - +1.5 1,963,114 - +0.6 1,963,203 - -0.8 1,963,284 + +0.9 1,963,284 + +1.5 1,963,285 - -0.6 1,963,285 - +0.1 1,963,285 - -0.3 1,963,309 - +0.1 1,963,362 + -0.5 1,963,362 + -1.3 1,963,363 - -1.5 1,963,363 - -1.9 1,963,363 - -1.4 1,963,395 + -0.0 1,963,396 - -0.2 1,963,403 + +1.3 1,963,608 + -0.1 1,963,610 + +0.1 1,963,610 + -0.9 1,963,611 - -0.3 1,963,627 + -1.3 1,963,627 + -0.1 1,963,628 - +0.8 1,963,628 - -1.0 1,963,628 - -0.4 1,963,629 + -1.7 1,963,714 + Psest_1859 0.22 +2.9 1,963,714 + Psest_1859 0.22 -0.9 1,963,714 + Psest_1859 0.22 -0.6 1,963,714 + Psest_1859 0.22 -1.0 1,963,715 - Psest_1859 0.22 -0.2 1,963,715 - Psest_1859 0.22 -0.7 1,963,734 + Psest_1859 0.25 -0.3 1,963,734 + Psest_1859 0.25 -0.7 1,963,778 + Psest_1859 0.32 -0.9 1,963,779 - Psest_1859 0.32 -2.1 1,963,779 - Psest_1859 0.32 -0.5 1,963,779 - Psest_1859 0.32 -0.2 1,963,795 + Psest_1859 0.34 +0.8 1,963,795 + Psest_1859 0.34 -0.5 1,963,937 + Psest_1859 0.56 -0.3 1,964,159 + Psest_1859 0.89 -2.7 1,964,159 + Psest_1859 0.89 +0.5 1,964,160 - Psest_1859 0.89 +1.8 1,964,160 - Psest_1859 0.89 -0.0 1,964,160 - Psest_1859 0.89 -1.0 1,964,250 - -0.8 1,964,268 + +0.6 1,964,545 + Psest_1860 0.38 -0.5 1,964,545 + Psest_1860 0.38 +2.0 1,964,546 - Psest_1860 0.38 -1.6 1,964,664 + Psest_1860 0.58 +1.8 1,964,881 + -0.1 1,964,946 + +0.2 1,964,947 - +0.8 1,964,997 + -0.8 1,964,997 + -0.4 1,964,997 + -2.4 1,964,997 + +0.3 1,964,998 - -0.9 1,964,998 - +1.1 1,964,998 - +0.2 1,964,998 - -0.6 1,965,118 - Psest_1861 0.13 -0.1 1,965,124 + Psest_1861 0.13 +0.8 1,965,124 + Psest_1861 0.13 +0.9 1,965,124 + Psest_1861 0.13 +1.3 1,965,125 - Psest_1861 0.13 +0.2 1,965,198 + Psest_1861 0.19 +0.7 1,965,198 + Psest_1861 0.19 -0.9 1,965,198 + Psest_1861 0.19 +0.1 1,965,199 - Psest_1861 0.19 -1.8
Or see this region's nucleotide sequence