Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0169 and Psest_0170 are separated by 28 nucleotides Psest_0170 and Psest_0172 are separated by 218 nucleotides Psest_0172 and Psest_0173 overlap by 4 nucleotides Psest_0173 and Psest_0174 are separated by 129 nucleotides Psest_0174 and Psest_0175 are separated by 16 nucleotides
Psest_0169: Psest_0169 - hypothetical protein, at 165,832 to 165,984
_0169
Psest_0170: Psest_0170 - 5,10-methenyltetrahydrofolate synthetase, at 166,013 to 166,615
_0170
Psest_0172: Psest_0172 - Uncharacterized protein conserved in bacteria, at 166,834 to 167,148
_0172
Psest_0173: Psest_0173 - TIGR02449 family protein, at 167,145 to 167,354
_0173
Psest_0174: Psest_0174 - Uncharacterized protein conserved in bacteria, at 167,484 to 168,038
_0174
Psest_0175: Psest_0175 - Xaa-Pro aminopeptidase, at 168,055 to 169,389
_0175
Position (kb) 166
167
168 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 165.840 kb on + strand at 165.840 kb on + strand at 165.840 kb on + strand at 165.840 kb on + strand at 165.841 kb on - strand at 165.849 kb on + strand, within Psest_0169 at 165.903 kb on + strand, within Psest_0169 at 166.023 kb on + strand at 166.023 kb on + strand at 166.058 kb on + strand at 166.058 kb on + strand at 166.058 kb on + strand at 166.059 kb on - strand at 166.059 kb on - strand at 166.059 kb on - strand at 166.059 kb on - strand at 166.059 kb on - strand at 166.173 kb on + strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.174 kb on - strand, within Psest_0170 at 166.383 kb on + strand, within Psest_0170 at 166.383 kb on + strand, within Psest_0170 at 166.383 kb on + strand, within Psest_0170 at 166.383 kb on + strand, within Psest_0170 at 166.383 kb on + strand, within Psest_0170 at 166.384 kb on - strand, within Psest_0170 at 166.384 kb on - strand, within Psest_0170 at 166.384 kb on - strand, within Psest_0170 at 166.456 kb on - strand, within Psest_0170 at 166.456 kb on - strand, within Psest_0170 at 166.456 kb on - strand, within Psest_0170 at 166.649 kb on + strand at 166.650 kb on - strand at 166.683 kb on + strand at 166.695 kb on + strand at 166.696 kb on - strand at 166.696 kb on - strand at 166.731 kb on + strand at 166.731 kb on + strand at 166.731 kb on + strand at 166.731 kb on + strand at 166.731 kb on + strand at 166.732 kb on - strand at 166.732 kb on - strand at 166.732 kb on - strand at 166.732 kb on - strand at 166.737 kb on + strand at 166.737 kb on + strand at 166.749 kb on + strand at 166.750 kb on - strand at 166.809 kb on + strand at 166.809 kb on + strand at 166.809 kb on + strand at 166.809 kb on + strand at 166.810 kb on - strand at 166.810 kb on - strand at 166.810 kb on - strand at 166.810 kb on - strand at 166.810 kb on - strand at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.897 kb on + strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 166.898 kb on - strand, within Psest_0172 at 167.102 kb on + strand, within Psest_0172 at 167.102 kb on + strand, within Psest_0172 at 167.102 kb on + strand, within Psest_0172 at 167.103 kb on - strand at 167.103 kb on - strand, within Psest_0172 at 167.103 kb on - strand, within Psest_0172 at 167.433 kb on - strand at 167.433 kb on - strand at 167.454 kb on - strand at 167.454 kb on - strand at 167.454 kb on - strand at 167.454 kb on - strand at 167.940 kb on + strand, within Psest_0174 at 167.940 kb on + strand, within Psest_0174 at 167.941 kb on - strand, within Psest_0174 at 167.941 kb on - strand, within Psest_0174 at 167.941 kb on - strand, within Psest_0174 at 167.958 kb on + strand, within Psest_0174 at 167.958 kb on + strand, within Psest_0174 at 167.959 kb on - strand, within Psest_0174 at 167.959 kb on - strand, within Psest_0174 at 167.959 kb on - strand, within Psest_0174 at 167.959 kb on - strand, within Psest_0174
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 165,840 + -1.8 165,840 + +1.0 165,840 + -0.8 165,840 + +0.6 165,841 - -2.6 165,849 + Psest_0169 0.11 -0.0 165,903 + Psest_0169 0.46 -0.0 166,023 + -2.1 166,023 + -1.1 166,058 + -3.2 166,058 + -2.5 166,058 + -1.3 166,059 - -3.2 166,059 - -1.4 166,059 - -4.3 166,059 - -0.2 166,059 - -3.1 166,173 + Psest_0170 0.27 -1.7 166,174 - Psest_0170 0.27 -2.7 166,174 - Psest_0170 0.27 -3.9 166,174 - Psest_0170 0.27 -1.1 166,174 - Psest_0170 0.27 -2.4 166,174 - Psest_0170 0.27 -3.0 166,174 - Psest_0170 0.27 -1.7 166,174 - Psest_0170 0.27 -4.4 166,174 - Psest_0170 0.27 -1.7 166,383 + Psest_0170 0.61 -1.9 166,383 + Psest_0170 0.61 -2.2 166,383 + Psest_0170 0.61 -0.7 166,383 + Psest_0170 0.61 -3.1 166,383 + Psest_0170 0.61 -2.3 166,384 - Psest_0170 0.62 -1.3 166,384 - Psest_0170 0.62 -1.0 166,384 - Psest_0170 0.62 -3.0 166,456 - Psest_0170 0.73 -0.0 166,456 - Psest_0170 0.73 -2.3 166,456 - Psest_0170 0.73 -2.6 166,649 + -2.4 166,650 - -0.4 166,683 + +0.2 166,695 + -0.8 166,696 - +0.3 166,696 - -0.1 166,731 + -1.5 166,731 + -0.4 166,731 + -1.5 166,731 + -1.2 166,731 + -2.3 166,732 - +2.3 166,732 - -0.6 166,732 - +0.2 166,732 - +0.0 166,737 + -2.0 166,737 + -1.0 166,749 + +1.1 166,750 - -0.9 166,809 + -3.4 166,809 + +0.2 166,809 + -0.6 166,809 + -0.2 166,810 - +0.2 166,810 - -0.5 166,810 - +1.7 166,810 - +0.1 166,810 - +1.0 166,897 + Psest_0172 0.20 -0.4 166,897 + Psest_0172 0.20 -0.2 166,897 + Psest_0172 0.20 -1.9 166,897 + Psest_0172 0.20 -0.4 166,897 + Psest_0172 0.20 -0.3 166,897 + Psest_0172 0.20 -0.0 166,897 + Psest_0172 0.20 -2.7 166,897 + Psest_0172 0.20 -1.9 166,897 + Psest_0172 0.20 -0.7 166,898 - Psest_0172 0.20 -0.1 166,898 - Psest_0172 0.20 -1.8 166,898 - Psest_0172 0.20 -1.1 166,898 - Psest_0172 0.20 -0.2 166,898 - Psest_0172 0.20 -1.2 166,898 - Psest_0172 0.20 -0.8 166,898 - Psest_0172 0.20 +0.8 166,898 - Psest_0172 0.20 -0.6 166,898 - Psest_0172 0.20 -3.2 166,898 - Psest_0172 0.20 +0.2 166,898 - Psest_0172 0.20 -0.6 167,102 + Psest_0172 0.85 -0.3 167,102 + Psest_0172 0.85 -1.0 167,102 + Psest_0172 0.85 -1.9 167,103 - +0.4 167,103 - Psest_0172 0.85 -2.4 167,103 - Psest_0172 0.85 -2.0 167,433 - +0.0 167,433 - -0.8 167,454 - +1.3 167,454 - -1.7 167,454 - -0.4 167,454 - -1.1 167,940 + Psest_0174 0.82 -0.3 167,940 + Psest_0174 0.82 -0.9 167,941 - Psest_0174 0.82 +2.2 167,941 - Psest_0174 0.82 +0.2 167,941 - Psest_0174 0.82 -0.0 167,958 + Psest_0174 0.85 -0.7 167,958 + Psest_0174 0.85 -1.8 167,959 - Psest_0174 0.86 -0.2 167,959 - Psest_0174 0.86 -0.6 167,959 - Psest_0174 0.86 +0.2 167,959 - Psest_0174 0.86 -0.4
Or see this region's nucleotide sequence