Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1554 and Psest_1555 are separated by 46 nucleotides Psest_1555 and Psest_1556 are separated by 275 nucleotides Psest_1556 and Psest_1557 are separated by 250 nucleotides
Psest_1554: Psest_1554 - Outer membrane protein and related peptidoglycan-associated (lipo)proteins, at 1,654,615 to 1,655,397
_1554
Psest_1555: Psest_1555 - hypothetical protein, at 1,655,444 to 1,655,836
_1555
Psest_1556: Psest_1556 - hypothetical protein, at 1,656,112 to 1,656,462
_1556
Psest_1557: Psest_1557 - Phosphoenolpyruvate carboxylase, at 1,656,713 to 1,659,352
_1557
Position (kb)
1656
1657 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1655.159 kb on + strand, within Psest_1554 at 1655.159 kb on + strand, within Psest_1554 at 1655.201 kb on + strand, within Psest_1554 at 1655.202 kb on - strand, within Psest_1554 at 1655.202 kb on - strand, within Psest_1554 at 1655.373 kb on + strand at 1655.373 kb on + strand at 1655.373 kb on + strand at 1655.373 kb on + strand at 1655.373 kb on + strand at 1655.373 kb on + strand at 1655.374 kb on - strand at 1655.377 kb on + strand at 1655.377 kb on + strand at 1655.377 kb on + strand at 1655.377 kb on + strand at 1655.377 kb on + strand at 1655.377 kb on + strand at 1655.411 kb on + strand at 1655.411 kb on + strand at 1655.411 kb on + strand at 1655.412 kb on - strand at 1655.418 kb on - strand at 1655.419 kb on + strand at 1655.419 kb on + strand at 1655.420 kb on - strand at 1655.420 kb on - strand at 1655.420 kb on - strand at 1655.461 kb on + strand at 1655.461 kb on + strand at 1655.461 kb on + strand at 1655.461 kb on + strand at 1655.461 kb on + strand at 1655.461 kb on + strand at 1655.462 kb on - strand at 1655.595 kb on + strand, within Psest_1555 at 1655.861 kb on + strand at 1655.898 kb on + strand at 1655.899 kb on - strand at 1655.972 kb on + strand at 1655.972 kb on + strand at 1656.324 kb on + strand, within Psest_1556 at 1656.395 kb on + strand, within Psest_1556 at 1656.395 kb on + strand, within Psest_1556 at 1656.395 kb on + strand, within Psest_1556 at 1656.396 kb on - strand, within Psest_1556 at 1656.396 kb on - strand, within Psest_1556 at 1656.396 kb on - strand, within Psest_1556 at 1656.396 kb on - strand, within Psest_1556 at 1656.466 kb on + strand at 1656.467 kb on - strand at 1656.467 kb on - strand at 1656.467 kb on - strand at 1656.467 kb on - strand at 1656.504 kb on - strand at 1656.582 kb on + strand at 1656.582 kb on + strand at 1656.653 kb on + strand at 1656.654 kb on - strand at 1656.654 kb on - strand at 1656.654 kb on - strand at 1656.750 kb on + strand at 1656.750 kb on + strand at 1656.750 kb on + strand at 1656.750 kb on + strand at 1656.750 kb on + strand at 1656.751 kb on - strand at 1656.751 kb on - strand at 1656.751 kb on - strand at 1656.751 kb on - strand at 1656.751 kb on - strand at 1656.751 kb on - strand at 1656.751 kb on - strand at 1656.751 kb on - strand at 1656.751 kb on - strand at 1656.965 kb on - strand at 1656.977 kb on + strand, within Psest_1557 at 1656.978 kb on - strand, within Psest_1557 at 1657.172 kb on + strand, within Psest_1557 at 1657.173 kb on - strand, within Psest_1557 at 1657.173 kb on - strand, within Psest_1557 at 1657.429 kb on - strand, within Psest_1557
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 1,655,159 + Psest_1554 0.69 -1.5 1,655,159 + Psest_1554 0.69 -0.9 1,655,201 + Psest_1554 0.75 +0.3 1,655,202 - Psest_1554 0.75 +0.1 1,655,202 - Psest_1554 0.75 +0.4 1,655,373 + -1.2 1,655,373 + +0.2 1,655,373 + +0.2 1,655,373 + -0.2 1,655,373 + -1.4 1,655,373 + -0.6 1,655,374 - -1.0 1,655,377 + -0.4 1,655,377 + -1.1 1,655,377 + -0.5 1,655,377 + -1.6 1,655,377 + +0.1 1,655,377 + -1.6 1,655,411 + -1.1 1,655,411 + -1.8 1,655,411 + -0.7 1,655,412 - -0.7 1,655,418 - +0.0 1,655,419 + -0.7 1,655,419 + -0.6 1,655,420 - -0.2 1,655,420 - +1.9 1,655,420 - -0.4 1,655,461 + -4.6 1,655,461 + -0.9 1,655,461 + -1.5 1,655,461 + +0.4 1,655,461 + -1.1 1,655,461 + -0.8 1,655,462 - -0.2 1,655,595 + Psest_1555 0.38 -0.6 1,655,861 + -1.0 1,655,898 + -0.2 1,655,899 - -0.9 1,655,972 + -1.9 1,655,972 + -1.3 1,656,324 + Psest_1556 0.60 -1.2 1,656,395 + Psest_1556 0.81 -0.2 1,656,395 + Psest_1556 0.81 +0.3 1,656,395 + Psest_1556 0.81 -0.4 1,656,396 - Psest_1556 0.81 -1.9 1,656,396 - Psest_1556 0.81 -0.6 1,656,396 - Psest_1556 0.81 +0.1 1,656,396 - Psest_1556 0.81 -1.4 1,656,466 + -1.0 1,656,467 - -2.5 1,656,467 - -1.6 1,656,467 - +0.7 1,656,467 - -0.5 1,656,504 - -0.1 1,656,582 + -0.8 1,656,582 + -0.6 1,656,653 + -0.8 1,656,654 - -0.8 1,656,654 - +0.4 1,656,654 - +1.3 1,656,750 + +1.2 1,656,750 + -0.1 1,656,750 + +1.3 1,656,750 + -1.5 1,656,750 + -1.2 1,656,751 - +0.5 1,656,751 - -0.4 1,656,751 - -0.1 1,656,751 - -1.6 1,656,751 - -0.2 1,656,751 - +0.1 1,656,751 - -0.7 1,656,751 - +1.1 1,656,751 - +0.0 1,656,965 - -0.1 1,656,977 + Psest_1557 0.10 +0.1 1,656,978 - Psest_1557 0.10 -0.2 1,657,172 + Psest_1557 0.17 -0.6 1,657,173 - Psest_1557 0.17 +0.7 1,657,173 - Psest_1557 0.17 +0.3 1,657,429 - Psest_1557 0.27 +0.5
Or see this region's nucleotide sequence