Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1297 and Psest_1298 are separated by 147 nucleotides Psest_1298 and Psest_1299 are separated by 75 nucleotides Psest_1299 and Psest_1300 are separated by 44 nucleotides Psest_1300 and Psest_1301 are separated by 48 nucleotides Psest_1301 and Psest_1302 are separated by 192 nucleotides Psest_1302 and Psest_1303 are separated by 15 nucleotides Psest_1303 and Psest_1304 are separated by 99 nucleotides
Psest_1297: Psest_1297 - glutamyl-tRNA synthetase, bacterial family, at 1,402,762 to 1,404,243
_1297
Psest_1298: Psest_1298 - tRNA_Ala_GGC, at 1,404,391 to 1,404,466
_1298
Psest_1299: Psest_1299 - tRNA_Glu_TTC, at 1,404,542 to 1,404,617
_1299
Psest_1300: Psest_1300 - tRNA_Ala_GGC, at 1,404,662 to 1,404,737
_1300
Psest_1301: Psest_1301 - tRNA_Glu_TTC, at 1,404,786 to 1,404,861
_1301
Psest_1302: Psest_1302 - Predicted thioesterase, at 1,405,054 to 1,405,503
_1302
Psest_1303: Psest_1303 - hypothetical protein, at 1,405,519 to 1,405,770
_1303
Psest_1304: Psest_1304 - Nucleotidyltransferase/DNA polymerase involved in DNA repair, at 1,405,870 to 1,406,934
_1304
Position (kb)
1405
1406 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1404.234 kb on + strand at 1404.297 kb on - strand at 1404.307 kb on - strand at 1404.619 kb on + strand at 1404.655 kb on + strand at 1404.655 kb on + strand at 1405.017 kb on + strand at 1405.017 kb on + strand at 1405.018 kb on - strand at 1405.135 kb on - strand, within Psest_1302 at 1405.144 kb on + strand, within Psest_1302 at 1405.144 kb on + strand, within Psest_1302 at 1405.145 kb on - strand, within Psest_1302 at 1405.211 kb on - strand, within Psest_1302 at 1405.211 kb on - strand, within Psest_1302 at 1405.242 kb on - strand, within Psest_1302 at 1405.259 kb on - strand, within Psest_1302 at 1405.342 kb on - strand, within Psest_1302 at 1405.501 kb on + strand at 1405.501 kb on + strand at 1405.501 kb on + strand at 1405.512 kb on + strand at 1405.591 kb on + strand, within Psest_1303 at 1405.592 kb on - strand, within Psest_1303 at 1405.592 kb on - strand, within Psest_1303 at 1405.592 kb on - strand, within Psest_1303 at 1405.592 kb on - strand, within Psest_1303 at 1405.592 kb on - strand, within Psest_1303 at 1405.757 kb on + strand at 1405.757 kb on + strand at 1405.757 kb on + strand at 1405.757 kb on + strand at 1405.757 kb on + strand at 1405.757 kb on + strand at 1405.758 kb on - strand at 1405.758 kb on - strand at 1405.758 kb on - strand at 1405.758 kb on - strand at 1405.758 kb on - strand at 1405.758 kb on - strand at 1405.805 kb on + strand at 1405.805 kb on + strand at 1405.805 kb on + strand at 1405.806 kb on - strand at 1405.806 kb on - strand at 1405.806 kb on - strand at 1405.806 kb on - strand at 1405.806 kb on - strand at 1405.921 kb on + strand at 1405.921 kb on + strand at 1405.921 kb on + strand at 1405.922 kb on - strand at 1405.922 kb on - strand at 1405.922 kb on - strand at 1405.922 kb on - strand at 1405.922 kb on - strand at 1406.011 kb on - strand, within Psest_1304 at 1406.011 kb on - strand, within Psest_1304 at 1406.020 kb on + strand, within Psest_1304 at 1406.021 kb on - strand, within Psest_1304 at 1406.078 kb on + strand, within Psest_1304 at 1406.079 kb on - strand, within Psest_1304 at 1406.161 kb on + strand, within Psest_1304 at 1406.161 kb on + strand, within Psest_1304 at 1406.161 kb on + strand, within Psest_1304 at 1406.161 kb on + strand, within Psest_1304 at 1406.161 kb on + strand, within Psest_1304 at 1406.161 kb on + strand, within Psest_1304 at 1406.161 kb on + strand, within Psest_1304 at 1406.161 kb on + strand, within Psest_1304 at 1406.161 kb on + strand, within Psest_1304 at 1406.161 kb on + strand, within Psest_1304 at 1406.162 kb on - strand, within Psest_1304 at 1406.162 kb on - strand, within Psest_1304 at 1406.162 kb on - strand, within Psest_1304 at 1406.162 kb on - strand, within Psest_1304 at 1406.162 kb on - strand, within Psest_1304 at 1406.162 kb on - strand, within Psest_1304 at 1406.162 kb on - strand, within Psest_1304 at 1406.162 kb on - strand, within Psest_1304 at 1406.162 kb on - strand, within Psest_1304 at 1406.162 kb on - strand, within Psest_1304 at 1406.200 kb on + strand, within Psest_1304
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 1,404,234 + -0.5 1,404,297 - -2.4 1,404,307 - -2.3 1,404,619 + +1.0 1,404,655 + +0.6 1,404,655 + +0.9 1,405,017 + -1.9 1,405,017 + +0.3 1,405,018 - -2.7 1,405,135 - Psest_1302 0.18 -0.3 1,405,144 + Psest_1302 0.20 -1.3 1,405,144 + Psest_1302 0.20 -1.1 1,405,145 - Psest_1302 0.20 -1.7 1,405,211 - Psest_1302 0.35 -0.5 1,405,211 - Psest_1302 0.35 -0.0 1,405,242 - Psest_1302 0.42 -2.2 1,405,259 - Psest_1302 0.46 -1.4 1,405,342 - Psest_1302 0.64 -1.8 1,405,501 + -2.6 1,405,501 + -1.0 1,405,501 + +0.1 1,405,512 + -1.1 1,405,591 + Psest_1303 0.29 -1.4 1,405,592 - Psest_1303 0.29 -0.2 1,405,592 - Psest_1303 0.29 -0.9 1,405,592 - Psest_1303 0.29 +0.6 1,405,592 - Psest_1303 0.29 -0.9 1,405,592 - Psest_1303 0.29 +0.4 1,405,757 + -1.0 1,405,757 + -3.4 1,405,757 + -1.6 1,405,757 + +0.0 1,405,757 + -1.6 1,405,757 + -0.8 1,405,758 - +0.8 1,405,758 - -1.1 1,405,758 - +0.0 1,405,758 - -2.2 1,405,758 - -0.6 1,405,758 - -4.2 1,405,805 + +0.2 1,405,805 + -1.5 1,405,805 + -1.5 1,405,806 - -1.4 1,405,806 - -1.2 1,405,806 - +0.6 1,405,806 - -0.3 1,405,806 - -1.6 1,405,921 + -1.8 1,405,921 + -0.2 1,405,921 + -2.0 1,405,922 - -2.5 1,405,922 - -3.1 1,405,922 - -1.3 1,405,922 - -0.5 1,405,922 - -0.9 1,406,011 - Psest_1304 0.13 +0.7 1,406,011 - Psest_1304 0.13 -0.7 1,406,020 + Psest_1304 0.14 -0.0 1,406,021 - Psest_1304 0.14 -1.1 1,406,078 + Psest_1304 0.20 -0.8 1,406,079 - Psest_1304 0.20 +0.1 1,406,161 + Psest_1304 0.27 -0.3 1,406,161 + Psest_1304 0.27 -0.3 1,406,161 + Psest_1304 0.27 -1.5 1,406,161 + Psest_1304 0.27 -2.6 1,406,161 + Psest_1304 0.27 -1.4 1,406,161 + Psest_1304 0.27 -0.3 1,406,161 + Psest_1304 0.27 +0.4 1,406,161 + Psest_1304 0.27 +0.6 1,406,161 + Psest_1304 0.27 -0.5 1,406,161 + Psest_1304 0.27 -0.6 1,406,162 - Psest_1304 0.27 -0.4 1,406,162 - Psest_1304 0.27 -1.4 1,406,162 - Psest_1304 0.27 -2.6 1,406,162 - Psest_1304 0.27 -0.7 1,406,162 - Psest_1304 0.27 -0.9 1,406,162 - Psest_1304 0.27 -0.1 1,406,162 - Psest_1304 0.27 -1.2 1,406,162 - Psest_1304 0.27 -0.7 1,406,162 - Psest_1304 0.27 -2.1 1,406,162 - Psest_1304 0.27 -1.9 1,406,200 + Psest_1304 0.31 -0.7
Or see this region's nucleotide sequence