Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1266 and Psest_1267 are separated by 4 nucleotides Psest_1267 and Psest_1268 are separated by 158 nucleotides Psest_1268 and Psest_1269 overlap by 13 nucleotides Psest_1269 and Psest_1270 are separated by 11 nucleotides
Psest_1266: Psest_1266 - ribosome-associated GTPase EngA, at 1,371,182 to 1,372,675
_1266
Psest_1267: Psest_1267 - serine O-acetyltransferase, at 1,372,680 to 1,373,474
_1267
Psest_1268: Psest_1268 - Aspartate/tyrosine/aromatic aminotransferase, at 1,373,633 to 1,374,781
_1268
Psest_1269: Psest_1269 - Predicted amidohydrolase, at 1,374,769 to 1,375,563
_1269
Psest_1270: Psest_1270 - hypothetical protein, at 1,375,575 to 1,375,766
_1270
Position (kb)
1373
1374
1375 Strain fitness (log2 ratio)
-2
-1
0
1 at 1372.965 kb on + strand, within Psest_1267 at 1372.965 kb on + strand, within Psest_1267 at 1372.965 kb on + strand, within Psest_1267 at 1372.965 kb on + strand, within Psest_1267 at 1372.965 kb on + strand, within Psest_1267 at 1372.965 kb on + strand, within Psest_1267 at 1372.966 kb on - strand, within Psest_1267 at 1372.966 kb on - strand, within Psest_1267 at 1373.166 kb on + strand, within Psest_1267 at 1373.166 kb on + strand, within Psest_1267 at 1373.166 kb on + strand, within Psest_1267 at 1373.167 kb on - strand, within Psest_1267 at 1373.265 kb on + strand, within Psest_1267 at 1373.265 kb on + strand, within Psest_1267 at 1373.265 kb on + strand, within Psest_1267 at 1373.265 kb on + strand, within Psest_1267 at 1373.265 kb on + strand, within Psest_1267 at 1373.266 kb on - strand, within Psest_1267 at 1373.266 kb on - strand, within Psest_1267 at 1373.266 kb on - strand, within Psest_1267 at 1373.486 kb on + strand at 1373.486 kb on + strand at 1373.487 kb on - strand at 1373.487 kb on - strand at 1373.487 kb on - strand at 1373.523 kb on + strand at 1373.524 kb on - strand at 1373.644 kb on + strand at 1373.645 kb on - strand at 1373.677 kb on + strand at 1373.758 kb on - strand, within Psest_1268 at 1373.973 kb on + strand, within Psest_1268 at 1373.996 kb on + strand, within Psest_1268 at 1374.015 kb on + strand, within Psest_1268 at 1374.206 kb on + strand, within Psest_1268 at 1374.206 kb on + strand, within Psest_1268 at 1374.206 kb on + strand, within Psest_1268 at 1374.206 kb on + strand, within Psest_1268 at 1374.207 kb on - strand, within Psest_1268 at 1374.207 kb on - strand, within Psest_1268 at 1374.207 kb on - strand, within Psest_1268 at 1374.207 kb on - strand, within Psest_1268 at 1374.207 kb on - strand, within Psest_1268 at 1374.207 kb on - strand, within Psest_1268 at 1374.207 kb on - strand, within Psest_1268 at 1374.228 kb on - strand, within Psest_1268 at 1374.287 kb on + strand, within Psest_1268 at 1374.287 kb on + strand, within Psest_1268 at 1374.353 kb on + strand, within Psest_1268 at 1374.404 kb on + strand, within Psest_1268 at 1374.498 kb on - strand, within Psest_1268 at 1374.572 kb on + strand, within Psest_1268 at 1374.572 kb on + strand, within Psest_1268 at 1374.572 kb on + strand, within Psest_1268 at 1374.572 kb on + strand, within Psest_1268 at 1374.768 kb on + strand at 1374.976 kb on + strand, within Psest_1269 at 1374.976 kb on + strand, within Psest_1269 at 1374.977 kb on - strand, within Psest_1269 at 1374.977 kb on - strand, within Psest_1269 at 1374.977 kb on - strand, within Psest_1269 at 1375.016 kb on + strand, within Psest_1269 at 1375.017 kb on - strand, within Psest_1269 at 1375.106 kb on - strand, within Psest_1269 at 1375.106 kb on - strand, within Psest_1269 at 1375.106 kb on - strand, within Psest_1269 at 1375.150 kb on + strand, within Psest_1269 at 1375.150 kb on + strand, within Psest_1269 at 1375.151 kb on - strand, within Psest_1269 at 1375.274 kb on - strand, within Psest_1269 at 1375.351 kb on + strand, within Psest_1269 at 1375.352 kb on - strand, within Psest_1269 at 1375.352 kb on - strand, within Psest_1269 at 1375.567 kb on + strand at 1375.568 kb on - strand at 1375.612 kb on + strand, within Psest_1270 at 1375.612 kb on + strand, within Psest_1270 at 1375.612 kb on + strand, within Psest_1270 at 1375.612 kb on + strand, within Psest_1270 at 1375.613 kb on - strand, within Psest_1270 at 1375.613 kb on - strand, within Psest_1270 at 1375.640 kb on - strand, within Psest_1270 at 1375.760 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 1,372,965 + Psest_1267 0.36 -0.6 1,372,965 + Psest_1267 0.36 -0.7 1,372,965 + Psest_1267 0.36 -2.1 1,372,965 + Psest_1267 0.36 -0.5 1,372,965 + Psest_1267 0.36 +0.2 1,372,965 + Psest_1267 0.36 -0.6 1,372,966 - Psest_1267 0.36 -1.8 1,372,966 - Psest_1267 0.36 -2.5 1,373,166 + Psest_1267 0.61 -1.5 1,373,166 + Psest_1267 0.61 -1.0 1,373,166 + Psest_1267 0.61 -0.7 1,373,167 - Psest_1267 0.61 +1.4 1,373,265 + Psest_1267 0.74 +0.2 1,373,265 + Psest_1267 0.74 +0.2 1,373,265 + Psest_1267 0.74 +0.0 1,373,265 + Psest_1267 0.74 -0.7 1,373,265 + Psest_1267 0.74 +0.2 1,373,266 - Psest_1267 0.74 -1.1 1,373,266 - Psest_1267 0.74 +0.1 1,373,266 - Psest_1267 0.74 +0.5 1,373,486 + +0.3 1,373,486 + -0.6 1,373,487 - +0.8 1,373,487 - -1.2 1,373,487 - -0.9 1,373,523 + -1.7 1,373,524 - -1.8 1,373,644 + -1.4 1,373,645 - -0.8 1,373,677 + -1.0 1,373,758 - Psest_1268 0.11 -0.7 1,373,973 + Psest_1268 0.30 -1.7 1,373,996 + Psest_1268 0.32 -2.0 1,374,015 + Psest_1268 0.33 -2.2 1,374,206 + Psest_1268 0.50 -1.1 1,374,206 + Psest_1268 0.50 -1.2 1,374,206 + Psest_1268 0.50 -1.4 1,374,206 + Psest_1268 0.50 -0.3 1,374,207 - Psest_1268 0.50 -0.6 1,374,207 - Psest_1268 0.50 -0.2 1,374,207 - Psest_1268 0.50 -0.3 1,374,207 - Psest_1268 0.50 -1.4 1,374,207 - Psest_1268 0.50 -1.0 1,374,207 - Psest_1268 0.50 +0.3 1,374,207 - Psest_1268 0.50 -0.4 1,374,228 - Psest_1268 0.52 -1.1 1,374,287 + Psest_1268 0.57 -1.0 1,374,287 + Psest_1268 0.57 -0.7 1,374,353 + Psest_1268 0.63 -1.3 1,374,404 + Psest_1268 0.67 -1.4 1,374,498 - Psest_1268 0.75 -0.2 1,374,572 + Psest_1268 0.82 -1.1 1,374,572 + Psest_1268 0.82 -2.3 1,374,572 + Psest_1268 0.82 -2.5 1,374,572 + Psest_1268 0.82 +0.4 1,374,768 + +0.9 1,374,976 + Psest_1269 0.26 -0.5 1,374,976 + Psest_1269 0.26 -0.1 1,374,977 - Psest_1269 0.26 -0.3 1,374,977 - Psest_1269 0.26 -0.5 1,374,977 - Psest_1269 0.26 -0.2 1,375,016 + Psest_1269 0.31 +0.6 1,375,017 - Psest_1269 0.31 +0.1 1,375,106 - Psest_1269 0.42 -0.4 1,375,106 - Psest_1269 0.42 +0.7 1,375,106 - Psest_1269 0.42 -0.9 1,375,150 + Psest_1269 0.48 +0.6 1,375,150 + Psest_1269 0.48 +0.5 1,375,151 - Psest_1269 0.48 -0.8 1,375,274 - Psest_1269 0.64 +0.4 1,375,351 + Psest_1269 0.73 -0.2 1,375,352 - Psest_1269 0.73 +0.5 1,375,352 - Psest_1269 0.73 +0.0 1,375,567 + -0.3 1,375,568 - -1.5 1,375,612 + Psest_1270 0.19 -1.3 1,375,612 + Psest_1270 0.19 -0.6 1,375,612 + Psest_1270 0.19 -1.0 1,375,612 + Psest_1270 0.19 -0.8 1,375,613 - Psest_1270 0.20 -1.7 1,375,613 - Psest_1270 0.20 +0.5 1,375,640 - Psest_1270 0.34 -0.3 1,375,760 + -0.3
Or see this region's nucleotide sequence