Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1217 and Psest_1218 are separated by 170 nucleotides Psest_1218 and Psest_1219 are separated by 88 nucleotides Psest_1219 and Psest_1220 are separated by 3 nucleotides Psest_1220 and Psest_1221 overlap by 4 nucleotides
Psest_1217: Psest_1217 - Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein, at 1,324,685 to 1,326,145
_1217
Psest_1218: Psest_1218 - Predicted endonuclease containing a URI domain, at 1,326,316 to 1,326,591
_1218
Psest_1219: Psest_1219 - Glutathione S-transferase, at 1,326,680 to 1,327,618
_1219
Psest_1220: Psest_1220 - hypothetical protein, at 1,327,622 to 1,328,464
_1220
Psest_1221: Psest_1221 - Uncharacterized conserved protein, at 1,328,461 to 1,329,279
_1221
Position (kb)
1326
1327
1328 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1325.775 kb on + strand, within Psest_1217 at 1325.776 kb on - strand, within Psest_1217 at 1325.776 kb on - strand, within Psest_1217 at 1325.785 kb on + strand, within Psest_1217 at 1325.985 kb on + strand, within Psest_1217 at 1325.985 kb on + strand, within Psest_1217 at 1325.986 kb on - strand, within Psest_1217 at 1325.986 kb on - strand, within Psest_1217 at 1325.986 kb on - strand, within Psest_1217 at 1325.986 kb on - strand, within Psest_1217 at 1325.989 kb on + strand, within Psest_1217 at 1325.989 kb on + strand, within Psest_1217 at 1325.990 kb on - strand, within Psest_1217 at 1325.990 kb on - strand, within Psest_1217 at 1326.035 kb on + strand at 1326.035 kb on + strand at 1326.036 kb on - strand at 1326.037 kb on + strand at 1326.038 kb on - strand at 1326.155 kb on - strand at 1326.156 kb on + strand at 1326.156 kb on + strand at 1326.156 kb on + strand at 1326.157 kb on - strand at 1326.207 kb on - strand at 1326.207 kb on - strand at 1326.228 kb on + strand at 1326.228 kb on + strand at 1326.228 kb on + strand at 1326.229 kb on - strand at 1326.274 kb on - strand at 1326.349 kb on + strand, within Psest_1218 at 1326.349 kb on + strand, within Psest_1218 at 1326.350 kb on - strand, within Psest_1218 at 1326.380 kb on - strand, within Psest_1218 at 1326.479 kb on - strand, within Psest_1218 at 1326.479 kb on - strand, within Psest_1218 at 1326.479 kb on - strand, within Psest_1218 at 1326.636 kb on - strand at 1326.659 kb on + strand at 1326.659 kb on + strand at 1326.660 kb on - strand at 1326.862 kb on + strand, within Psest_1219 at 1326.866 kb on + strand, within Psest_1219 at 1326.866 kb on + strand, within Psest_1219 at 1326.866 kb on + strand, within Psest_1219 at 1326.866 kb on + strand, within Psest_1219 at 1326.867 kb on - strand, within Psest_1219 at 1326.867 kb on - strand, within Psest_1219 at 1326.867 kb on - strand, within Psest_1219 at 1327.157 kb on - strand, within Psest_1219 at 1327.157 kb on - strand, within Psest_1219 at 1327.157 kb on - strand, within Psest_1219 at 1327.515 kb on + strand, within Psest_1219 at 1327.516 kb on - strand, within Psest_1219 at 1327.785 kb on - strand, within Psest_1220 at 1327.893 kb on + strand, within Psest_1220 at 1327.894 kb on - strand, within Psest_1220 at 1327.894 kb on - strand, within Psest_1220 at 1327.894 kb on - strand, within Psest_1220 at 1327.894 kb on - strand, within Psest_1220 at 1327.984 kb on - strand, within Psest_1220 at 1327.998 kb on + strand, within Psest_1220 at 1328.088 kb on + strand, within Psest_1220 at 1328.088 kb on + strand, within Psest_1220 at 1328.088 kb on + strand, within Psest_1220 at 1328.088 kb on + strand, within Psest_1220 at 1328.088 kb on + strand, within Psest_1220 at 1328.089 kb on - strand, within Psest_1220 at 1328.089 kb on - strand, within Psest_1220 at 1328.089 kb on - strand, within Psest_1220 at 1328.089 kb on - strand, within Psest_1220 at 1328.089 kb on - strand, within Psest_1220 at 1328.089 kb on - strand, within Psest_1220 at 1328.202 kb on + strand, within Psest_1220 at 1328.202 kb on + strand, within Psest_1220 at 1328.202 kb on + strand, within Psest_1220 at 1328.203 kb on - strand, within Psest_1220 at 1328.203 kb on - strand, within Psest_1220 at 1328.435 kb on + strand at 1328.436 kb on - strand at 1328.594 kb on - strand, within Psest_1221 at 1328.600 kb on + strand, within Psest_1221 at 1328.600 kb on + strand, within Psest_1221 at 1328.600 kb on + strand, within Psest_1221 at 1328.601 kb on - strand, within Psest_1221
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 1,325,775 + Psest_1217 0.75 +0.1 1,325,776 - Psest_1217 0.75 -0.3 1,325,776 - Psest_1217 0.75 -0.3 1,325,785 + Psest_1217 0.75 -2.0 1,325,985 + Psest_1217 0.89 -0.6 1,325,985 + Psest_1217 0.89 -0.3 1,325,986 - Psest_1217 0.89 +0.7 1,325,986 - Psest_1217 0.89 +1.0 1,325,986 - Psest_1217 0.89 +0.3 1,325,986 - Psest_1217 0.89 -0.2 1,325,989 + Psest_1217 0.89 -0.9 1,325,989 + Psest_1217 0.89 -1.8 1,325,990 - Psest_1217 0.89 -0.6 1,325,990 - Psest_1217 0.89 -0.2 1,326,035 + -1.3 1,326,035 + -1.2 1,326,036 - -1.3 1,326,037 + -1.4 1,326,038 - -1.0 1,326,155 - -0.0 1,326,156 + +0.9 1,326,156 + -0.9 1,326,156 + -0.8 1,326,157 - -0.1 1,326,207 - -0.6 1,326,207 - +0.7 1,326,228 + +0.1 1,326,228 + -1.3 1,326,228 + +0.8 1,326,229 - -1.2 1,326,274 - -0.7 1,326,349 + Psest_1218 0.12 -0.7 1,326,349 + Psest_1218 0.12 -0.3 1,326,350 - Psest_1218 0.12 -0.6 1,326,380 - Psest_1218 0.23 -0.3 1,326,479 - Psest_1218 0.59 +1.1 1,326,479 - Psest_1218 0.59 +0.5 1,326,479 - Psest_1218 0.59 -0.9 1,326,636 - -0.6 1,326,659 + -0.1 1,326,659 + +0.9 1,326,660 - -1.3 1,326,862 + Psest_1219 0.19 +0.9 1,326,866 + Psest_1219 0.20 +0.1 1,326,866 + Psest_1219 0.20 +0.0 1,326,866 + Psest_1219 0.20 +1.6 1,326,866 + Psest_1219 0.20 +0.2 1,326,867 - Psest_1219 0.20 +1.6 1,326,867 - Psest_1219 0.20 -1.7 1,326,867 - Psest_1219 0.20 -0.2 1,327,157 - Psest_1219 0.51 +0.0 1,327,157 - Psest_1219 0.51 -0.9 1,327,157 - Psest_1219 0.51 -0.5 1,327,515 + Psest_1219 0.89 -2.2 1,327,516 - Psest_1219 0.89 -1.3 1,327,785 - Psest_1220 0.19 -0.8 1,327,893 + Psest_1220 0.32 -0.9 1,327,894 - Psest_1220 0.32 -1.5 1,327,894 - Psest_1220 0.32 -0.9 1,327,894 - Psest_1220 0.32 -0.5 1,327,894 - Psest_1220 0.32 -0.6 1,327,984 - Psest_1220 0.43 +0.0 1,327,998 + Psest_1220 0.45 +0.2 1,328,088 + Psest_1220 0.55 +1.7 1,328,088 + Psest_1220 0.55 -0.1 1,328,088 + Psest_1220 0.55 +0.7 1,328,088 + Psest_1220 0.55 -1.9 1,328,088 + Psest_1220 0.55 +0.8 1,328,089 - Psest_1220 0.55 -0.9 1,328,089 - Psest_1220 0.55 -0.2 1,328,089 - Psest_1220 0.55 -2.1 1,328,089 - Psest_1220 0.55 -1.0 1,328,089 - Psest_1220 0.55 -3.3 1,328,089 - Psest_1220 0.55 -0.1 1,328,202 + Psest_1220 0.69 +1.9 1,328,202 + Psest_1220 0.69 +0.6 1,328,202 + Psest_1220 0.69 +0.5 1,328,203 - Psest_1220 0.69 -1.5 1,328,203 - Psest_1220 0.69 -1.4 1,328,435 + -2.0 1,328,436 - -2.8 1,328,594 - Psest_1221 0.16 -0.0 1,328,600 + Psest_1221 0.17 -0.2 1,328,600 + Psest_1221 0.17 +0.8 1,328,600 + Psest_1221 0.17 +0.0 1,328,601 - Psest_1221 0.17 +0.7
Or see this region's nucleotide sequence