Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1198 and Psest_1199 are separated by 60 nucleotides Psest_1199 and Psest_1200 overlap by 4 nucleotides
Psest_1198: Psest_1198 - allantoicase, at 1,301,672 to 1,302,667
_1198
Psest_1199: Psest_1199 - OHCU decarboxylase, at 1,302,728 to 1,303,243
_1199
Psest_1200: Psest_1200 - putative urate catabolism protein, at 1,303,240 to 1,304,172
_1200
Position (kb)
1302
1303
1304 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1301.820 kb on - strand, within Psest_1198 at 1301.820 kb on - strand, within Psest_1198 at 1301.878 kb on - strand, within Psest_1198 at 1301.878 kb on - strand, within Psest_1198 at 1301.878 kb on - strand, within Psest_1198 at 1302.348 kb on + strand, within Psest_1198 at 1302.348 kb on + strand, within Psest_1198 at 1302.348 kb on + strand, within Psest_1198 at 1302.349 kb on - strand, within Psest_1198 at 1302.349 kb on - strand, within Psest_1198 at 1302.349 kb on - strand, within Psest_1198 at 1302.349 kb on - strand, within Psest_1198 at 1302.349 kb on - strand, within Psest_1198 at 1302.349 kb on - strand, within Psest_1198 at 1302.460 kb on - strand, within Psest_1198 at 1302.636 kb on + strand at 1302.700 kb on + strand at 1302.700 kb on + strand at 1302.700 kb on + strand at 1302.701 kb on - strand at 1302.701 kb on - strand at 1302.701 kb on - strand at 1302.729 kb on + strand at 1302.729 kb on + strand at 1302.729 kb on + strand at 1302.729 kb on + strand at 1302.729 kb on + strand at 1302.729 kb on + strand at 1302.730 kb on - strand at 1302.894 kb on + strand, within Psest_1199 at 1302.895 kb on - strand, within Psest_1199 at 1302.895 kb on - strand, within Psest_1199 at 1302.895 kb on - strand, within Psest_1199 at 1302.895 kb on - strand, within Psest_1199 at 1303.107 kb on + strand, within Psest_1199 at 1303.170 kb on + strand, within Psest_1199 at 1303.170 kb on + strand, within Psest_1199 at 1303.170 kb on + strand, within Psest_1199 at 1303.443 kb on - strand, within Psest_1200 at 1303.469 kb on + strand, within Psest_1200 at 1303.470 kb on - strand, within Psest_1200 at 1303.541 kb on + strand, within Psest_1200 at 1303.541 kb on + strand, within Psest_1200 at 1303.607 kb on + strand, within Psest_1200 at 1303.607 kb on + strand, within Psest_1200 at 1303.608 kb on - strand, within Psest_1200 at 1303.608 kb on - strand, within Psest_1200 at 1303.623 kb on - strand, within Psest_1200 at 1303.682 kb on + strand, within Psest_1200 at 1303.683 kb on - strand, within Psest_1200 at 1303.683 kb on - strand, within Psest_1200 at 1303.683 kb on - strand, within Psest_1200 at 1303.949 kb on + strand, within Psest_1200 at 1303.949 kb on + strand, within Psest_1200 at 1303.949 kb on + strand, within Psest_1200 at 1303.949 kb on + strand, within Psest_1200 at 1303.949 kb on + strand, within Psest_1200 at 1304.069 kb on + strand, within Psest_1200 at 1304.070 kb on - strand, within Psest_1200 at 1304.159 kb on + strand at 1304.188 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 1,301,820 - Psest_1198 0.15 -1.9 1,301,820 - Psest_1198 0.15 -1.9 1,301,878 - Psest_1198 0.21 -1.5 1,301,878 - Psest_1198 0.21 -0.7 1,301,878 - Psest_1198 0.21 -0.9 1,302,348 + Psest_1198 0.68 -0.7 1,302,348 + Psest_1198 0.68 -0.6 1,302,348 + Psest_1198 0.68 -0.8 1,302,349 - Psest_1198 0.68 -0.7 1,302,349 - Psest_1198 0.68 -1.0 1,302,349 - Psest_1198 0.68 +0.2 1,302,349 - Psest_1198 0.68 -0.5 1,302,349 - Psest_1198 0.68 -0.4 1,302,349 - Psest_1198 0.68 +1.1 1,302,460 - Psest_1198 0.79 -2.5 1,302,636 + +0.9 1,302,700 + -0.5 1,302,700 + -0.1 1,302,700 + +0.8 1,302,701 - -2.1 1,302,701 - -0.8 1,302,701 - -0.7 1,302,729 + +0.0 1,302,729 + +0.3 1,302,729 + +0.0 1,302,729 + -0.1 1,302,729 + +1.1 1,302,729 + +0.3 1,302,730 - -2.1 1,302,894 + Psest_1199 0.32 -0.7 1,302,895 - Psest_1199 0.32 -3.4 1,302,895 - Psest_1199 0.32 +2.0 1,302,895 - Psest_1199 0.32 -1.4 1,302,895 - Psest_1199 0.32 -0.3 1,303,107 + Psest_1199 0.73 +1.0 1,303,170 + Psest_1199 0.86 -0.1 1,303,170 + Psest_1199 0.86 -0.9 1,303,170 + Psest_1199 0.86 +0.4 1,303,443 - Psest_1200 0.22 +1.2 1,303,469 + Psest_1200 0.25 -0.6 1,303,470 - Psest_1200 0.25 -2.7 1,303,541 + Psest_1200 0.32 -0.0 1,303,541 + Psest_1200 0.32 -0.6 1,303,607 + Psest_1200 0.39 -0.9 1,303,607 + Psest_1200 0.39 -1.3 1,303,608 - Psest_1200 0.39 -0.1 1,303,608 - Psest_1200 0.39 +0.1 1,303,623 - Psest_1200 0.41 +1.4 1,303,682 + Psest_1200 0.47 -1.0 1,303,683 - Psest_1200 0.47 +0.6 1,303,683 - Psest_1200 0.47 -1.2 1,303,683 - Psest_1200 0.47 +0.8 1,303,949 + Psest_1200 0.76 -1.2 1,303,949 + Psest_1200 0.76 -3.1 1,303,949 + Psest_1200 0.76 -2.0 1,303,949 + Psest_1200 0.76 -1.4 1,303,949 + Psest_1200 0.76 -3.3 1,304,069 + Psest_1200 0.89 -0.4 1,304,070 - Psest_1200 0.89 -0.1 1,304,159 + -0.6 1,304,188 - -0.3
Or see this region's nucleotide sequence