Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1130 and Psest_1131 are separated by 123 nucleotides Psest_1131 and Psest_1132 overlap by 4 nucleotides Psest_1132 and Psest_1133 are separated by 172 nucleotides Psest_1133 and Psest_1134 are separated by 119 nucleotides
Psest_1130: Psest_1130 - glutamate N-acetyltransferase/amino-acid acetyltransferase, at 1,226,912 to 1,228,129
_1130
Psest_1131: Psest_1131 - Glutathione S-transferase, at 1,228,253 to 1,228,885
_1131
Psest_1132: Psest_1132 - mutator mutT protein, at 1,228,882 to 1,229,820
_1132
Psest_1133: Psest_1133 - peroxiredoxin, at 1,229,993 to 1,230,556
_1133
Psest_1134: Psest_1134 - alkyl hydroperoxide reductase, F subunit, at 1,230,676 to 1,232,238
_1134
Position (kb)
1228
1229
1230 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1228.098 kb on - strand at 1228.098 kb on - strand at 1228.144 kb on + strand at 1228.145 kb on - strand at 1228.148 kb on + strand at 1228.148 kb on + strand at 1228.148 kb on + strand at 1228.149 kb on - strand at 1228.149 kb on - strand at 1228.149 kb on - strand at 1228.149 kb on - strand at 1228.149 kb on - strand at 1228.149 kb on - strand at 1228.149 kb on - strand at 1228.286 kb on + strand at 1228.286 kb on + strand at 1228.287 kb on - strand at 1228.287 kb on - strand at 1228.287 kb on - strand at 1228.287 kb on - strand at 1228.345 kb on - strand, within Psest_1131 at 1228.397 kb on + strand, within Psest_1131 at 1228.397 kb on + strand, within Psest_1131 at 1228.472 kb on + strand, within Psest_1131 at 1228.473 kb on - strand, within Psest_1131 at 1228.503 kb on + strand, within Psest_1131 at 1228.504 kb on - strand, within Psest_1131 at 1228.504 kb on - strand, within Psest_1131 at 1228.504 kb on - strand, within Psest_1131 at 1228.543 kb on + strand, within Psest_1131 at 1228.544 kb on - strand, within Psest_1131 at 1228.544 kb on - strand, within Psest_1131 at 1228.544 kb on - strand, within Psest_1131 at 1228.544 kb on - strand, within Psest_1131 at 1228.712 kb on + strand, within Psest_1131 at 1228.748 kb on + strand, within Psest_1131 at 1228.748 kb on + strand, within Psest_1131 at 1228.748 kb on + strand, within Psest_1131 at 1228.748 kb on + strand, within Psest_1131 at 1228.749 kb on - strand, within Psest_1131 at 1228.749 kb on - strand, within Psest_1131 at 1228.749 kb on - strand, within Psest_1131 at 1228.749 kb on - strand, within Psest_1131 at 1228.749 kb on - strand, within Psest_1131 at 1228.811 kb on + strand, within Psest_1131 at 1228.812 kb on - strand, within Psest_1131 at 1228.812 kb on - strand, within Psest_1131 at 1228.812 kb on - strand, within Psest_1131 at 1228.812 kb on - strand, within Psest_1131 at 1228.812 kb on - strand, within Psest_1131 at 1228.934 kb on + strand at 1228.934 kb on + strand at 1228.934 kb on + strand at 1228.935 kb on - strand at 1228.935 kb on - strand at 1229.218 kb on + strand, within Psest_1132 at 1229.218 kb on + strand, within Psest_1132 at 1229.218 kb on + strand, within Psest_1132 at 1229.219 kb on - strand, within Psest_1132 at 1229.392 kb on + strand, within Psest_1132 at 1229.393 kb on - strand, within Psest_1132 at 1229.393 kb on - strand, within Psest_1132 at 1229.615 kb on + strand, within Psest_1132 at 1229.705 kb on + strand, within Psest_1132 at 1229.726 kb on - strand, within Psest_1132 at 1229.836 kb on + strand at 1229.836 kb on + strand at 1229.836 kb on + strand at 1229.836 kb on + strand at 1229.837 kb on - strand at 1229.837 kb on - strand at 1229.837 kb on - strand at 1229.837 kb on - strand at 1229.861 kb on + strand at 1229.861 kb on + strand at 1229.875 kb on + strand at 1229.945 kb on + strand at 1229.945 kb on + strand at 1229.950 kb on + strand at 1230.554 kb on + strand at 1230.554 kb on + strand at 1230.554 kb on + strand at 1230.554 kb on + strand at 1230.555 kb on - strand at 1230.643 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 1,228,098 - +1.4 1,228,098 - -0.9 1,228,144 + +0.6 1,228,145 - -0.1 1,228,148 + +2.0 1,228,148 + +2.2 1,228,148 + +1.7 1,228,149 - -0.0 1,228,149 - +0.8 1,228,149 - -0.4 1,228,149 - +0.2 1,228,149 - +0.3 1,228,149 - -1.4 1,228,149 - -0.0 1,228,286 + -1.3 1,228,286 + +0.6 1,228,287 - +0.2 1,228,287 - -0.7 1,228,287 - +0.9 1,228,287 - +1.1 1,228,345 - Psest_1131 0.15 +1.1 1,228,397 + Psest_1131 0.23 -0.7 1,228,397 + Psest_1131 0.23 -0.5 1,228,472 + Psest_1131 0.35 -3.3 1,228,473 - Psest_1131 0.35 +1.9 1,228,503 + Psest_1131 0.39 +0.0 1,228,504 - Psest_1131 0.40 +0.7 1,228,504 - Psest_1131 0.40 +0.5 1,228,504 - Psest_1131 0.40 +0.6 1,228,543 + Psest_1131 0.46 -0.7 1,228,544 - Psest_1131 0.46 -0.0 1,228,544 - Psest_1131 0.46 -1.1 1,228,544 - Psest_1131 0.46 -1.0 1,228,544 - Psest_1131 0.46 +0.8 1,228,712 + Psest_1131 0.73 +0.7 1,228,748 + Psest_1131 0.78 +0.2 1,228,748 + Psest_1131 0.78 +0.9 1,228,748 + Psest_1131 0.78 -0.9 1,228,748 + Psest_1131 0.78 +0.5 1,228,749 - Psest_1131 0.78 -0.2 1,228,749 - Psest_1131 0.78 +0.5 1,228,749 - Psest_1131 0.78 -0.1 1,228,749 - Psest_1131 0.78 -0.6 1,228,749 - Psest_1131 0.78 +1.4 1,228,811 + Psest_1131 0.88 -0.0 1,228,812 - Psest_1131 0.88 +0.3 1,228,812 - Psest_1131 0.88 -1.4 1,228,812 - Psest_1131 0.88 +0.2 1,228,812 - Psest_1131 0.88 -0.3 1,228,812 - Psest_1131 0.88 -0.7 1,228,934 + -2.5 1,228,934 + +0.5 1,228,934 + +0.5 1,228,935 - +0.7 1,228,935 - -2.2 1,229,218 + Psest_1132 0.36 -1.3 1,229,218 + Psest_1132 0.36 -1.9 1,229,218 + Psest_1132 0.36 -0.8 1,229,219 - Psest_1132 0.36 -1.2 1,229,392 + Psest_1132 0.54 -0.5 1,229,393 - Psest_1132 0.54 +0.0 1,229,393 - Psest_1132 0.54 -3.1 1,229,615 + Psest_1132 0.78 -2.6 1,229,705 + Psest_1132 0.88 +1.1 1,229,726 - Psest_1132 0.90 -2.4 1,229,836 + -0.0 1,229,836 + +1.2 1,229,836 + -0.3 1,229,836 + -1.3 1,229,837 - +1.0 1,229,837 - -2.0 1,229,837 - -0.7 1,229,837 - -0.5 1,229,861 + +0.1 1,229,861 + -1.4 1,229,875 + -0.0 1,229,945 + -0.0 1,229,945 + -0.3 1,229,950 + -1.9 1,230,554 + +1.9 1,230,554 + +1.2 1,230,554 + +1.8 1,230,554 + +2.2 1,230,555 - +0.4 1,230,643 - +1.2
Or see this region's nucleotide sequence