Experiment: Def Carbenicillin 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1127 and Psest_1128 are separated by 120 nucleotides Psest_1128 and Psest_1129 are separated by 272 nucleotides Psest_1129 and Psest_1130 are separated by 114 nucleotides
Psest_1127: Psest_1127 - hypothetical protein, at 1,222,728 to 1,223,192
_1127
Psest_1128: Psest_1128 - Uncharacterized protein conserved in bacteria, at 1,223,313 to 1,223,768
_1128
Psest_1129: Psest_1129 - preprotein translocase, SecA subunit, at 1,224,041 to 1,226,797
_1129
Psest_1130: Psest_1130 - glutamate N-acetyltransferase/amino-acid acetyltransferase, at 1,226,912 to 1,228,129
_1130
Position (kb)
1224
1225
1226
1227 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1223.043 kb on + strand, within Psest_1127 at 1223.155 kb on - strand at 1223.155 kb on - strand at 1223.303 kb on + strand at 1223.304 kb on - strand at 1223.778 kb on + strand at 1223.778 kb on + strand at 1223.818 kb on - strand at 1223.818 kb on - strand at 1226.768 kb on + strand at 1226.768 kb on + strand at 1226.795 kb on + strand at 1226.796 kb on - strand at 1226.890 kb on + strand at 1226.890 kb on + strand at 1226.890 kb on + strand at 1226.890 kb on + strand at 1226.890 kb on + strand at 1226.891 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Def Carbenicillin 0.008 mg/ml remove 1,223,043 + Psest_1127 0.68 -0.9 1,223,155 - -0.8 1,223,155 - -1.9 1,223,303 + +0.5 1,223,304 - -1.3 1,223,778 + -2.5 1,223,778 + -0.4 1,223,818 - -1.3 1,223,818 - -0.8 1,226,768 + +1.2 1,226,768 + +2.7 1,226,795 + +1.8 1,226,796 - -1.6 1,226,890 + +0.3 1,226,890 + +0.7 1,226,890 + -0.7 1,226,890 + +0.4 1,226,890 + -0.3 1,226,891 - +2.0
Or see this region's nucleotide sequence