Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0022

Experiment: Def Benzethonium chloride 0.008 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0021 and Psest_0022 overlap by 4 nucleotidesPsest_0022 and Psest_0023 are separated by 55 nucleotidesPsest_0023 and Psest_0024 are separated by 148 nucleotides Psest_0021: Psest_0021 - ribosomal RNA small subunit methyltransferase RsmB, at 21,703 to 23,025 _0021 Psest_0022: Psest_0022 - methionyl-tRNA formyltransferase, at 23,022 to 23,966 _0022 Psest_0023: Psest_0023 - peptide deformylase, at 24,022 to 24,528 _0023 Psest_0024: Psest_0024 - LysM domain., at 24,677 to 25,702 _0024 Position (kb) 23 24Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 22.147 kb on + strand, within Psest_0021at 22.148 kb on - strand, within Psest_0021at 22.148 kb on - strand, within Psest_0021at 22.148 kb on - strand, within Psest_0021at 22.148 kb on - strand, within Psest_0021at 22.148 kb on - strand, within Psest_0021at 22.148 kb on - strand, within Psest_0021at 22.473 kb on - strand, within Psest_0021at 22.770 kb on - strand, within Psest_0021at 22.770 kb on - strand, within Psest_0021at 22.770 kb on - strand, within Psest_0021at 22.781 kb on + strand, within Psest_0021at 22.782 kb on - strand, within Psest_0021at 24.529 kb on - strandat 24.529 kb on - strandat 24.555 kb on - strandat 24.555 kb on - strandat 24.574 kb on - strandat 24.574 kb on - strandat 24.574 kb on - strandat 24.659 kb on - strandat 24.659 kb on - strandat 24.659 kb on - strandat 24.659 kb on - strandat 24.659 kb on - strandat 24.662 kb on - strandat 24.662 kb on - strandat 24.662 kb on - strandat 24.768 kb on - strandat 24.769 kb on + strandat 24.769 kb on + strandat 24.769 kb on + strandat 24.769 kb on + strandat 24.884 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.911 kb on + strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.912 kb on - strand, within Psest_0024at 24.936 kb on + strand, within Psest_0024at 24.937 kb on - strand, within Psest_0024

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Per-strain Table

Position Strand Gene LocusTag Fraction Def Benzethonium chloride 0.008 mM
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22,147 + Psest_0021 0.34 +0.8
22,148 - Psest_0021 0.34 +0.2
22,148 - Psest_0021 0.34 -0.4
22,148 - Psest_0021 0.34 -0.9
22,148 - Psest_0021 0.34 +0.5
22,148 - Psest_0021 0.34 +0.7
22,148 - Psest_0021 0.34 -1.3
22,473 - Psest_0021 0.58 -0.1
22,770 - Psest_0021 0.81 +0.7
22,770 - Psest_0021 0.81 +0.4
22,770 - Psest_0021 0.81 -0.6
22,781 + Psest_0021 0.81 +0.2
22,782 - Psest_0021 0.82 -0.4
24,529 - -2.4
24,529 - -2.0
24,555 - -3.8
24,555 - -4.3
24,574 - -3.7
24,574 - -3.4
24,574 - -2.8
24,659 - -3.7
24,659 - -2.4
24,659 - -3.0
24,659 - -2.7
24,659 - -1.4
24,662 - -2.3
24,662 - -2.6
24,662 - -3.2
24,768 - -1.8
24,769 + -1.9
24,769 + -2.8
24,769 + -3.9
24,769 + -1.2
24,884 + Psest_0024 0.20 -1.7
24,911 + Psest_0024 0.23 -2.1
24,911 + Psest_0024 0.23 +0.2
24,911 + Psest_0024 0.23 -0.8
24,911 + Psest_0024 0.23 -2.8
24,911 + Psest_0024 0.23 -1.0
24,911 + Psest_0024 0.23 -2.3
24,911 + Psest_0024 0.23 -2.9
24,911 + Psest_0024 0.23 -3.0
24,911 + Psest_0024 0.23 -2.4
24,911 + Psest_0024 0.23 -2.3
24,911 + Psest_0024 0.23 -3.1
24,912 - Psest_0024 0.23 -1.0
24,912 - Psest_0024 0.23 -1.0
24,912 - Psest_0024 0.23 -2.7
24,912 - Psest_0024 0.23 -1.9
24,912 - Psest_0024 0.23 -4.0
24,912 - Psest_0024 0.23 -3.2
24,912 - Psest_0024 0.23 -2.8
24,912 - Psest_0024 0.23 -3.1
24,912 - Psest_0024 0.23 -3.0
24,912 - Psest_0024 0.23 -0.5
24,912 - Psest_0024 0.23 -2.6
24,912 - Psest_0024 0.23 -3.2
24,936 + Psest_0024 0.25 -0.6
24,937 - Psest_0024 0.25 -1.7

Or see this region's nucleotide sequence