Experiment: RCH2 defined media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0891 and Psest_0892 are separated by 60 nucleotides Psest_0892 and Psest_0893 are separated by 108 nucleotides Psest_0893 and Psest_0895 are separated by 568 nucleotides
Psest_0891: Psest_0891 - Uncharacterized protein conserved in bacteria, at 968,090 to 968,314
_0891
Psest_0892: Psest_0892 - Short-chain alcohol dehydrogenase of unknown specificity, at 968,375 to 969,154
_0892
Psest_0893: Psest_0893 - Transcriptional regulator, at 969,263 to 970,177
_0893
Psest_0895: Psest_0895 - Threonine dehydrogenase and related Zn-dependent dehydrogenases, at 970,746 to 971,885
_0895
Position (kb) 969
970
971 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 968.335 kb on + strand at 968.377 kb on - strand at 968.413 kb on + strand at 968.490 kb on + strand, within Psest_0892 at 968.490 kb on + strand, within Psest_0892 at 968.490 kb on + strand, within Psest_0892 at 968.490 kb on + strand, within Psest_0892 at 968.490 kb on + strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.491 kb on - strand, within Psest_0892 at 968.620 kb on + strand, within Psest_0892 at 968.621 kb on - strand, within Psest_0892 at 968.649 kb on + strand, within Psest_0892 at 968.758 kb on - strand, within Psest_0892 at 968.780 kb on - strand, within Psest_0892 at 968.858 kb on + strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.859 kb on - strand, within Psest_0892 at 968.874 kb on + strand, within Psest_0892 at 968.874 kb on + strand, within Psest_0892 at 968.875 kb on - strand, within Psest_0892 at 968.923 kb on + strand, within Psest_0892 at 968.923 kb on + strand, within Psest_0892 at 968.923 kb on + strand, within Psest_0892 at 968.924 kb on - strand, within Psest_0892 at 968.924 kb on - strand, within Psest_0892 at 968.924 kb on - strand, within Psest_0892 at 968.992 kb on + strand, within Psest_0892 at 968.993 kb on - strand, within Psest_0892 at 969.018 kb on - strand, within Psest_0892 at 969.145 kb on - strand at 969.184 kb on + strand at 969.185 kb on - strand at 969.185 kb on - strand at 969.185 kb on - strand at 969.206 kb on - strand at 969.230 kb on - strand at 969.269 kb on - strand at 969.287 kb on - strand at 969.316 kb on + strand at 969.316 kb on + strand at 969.316 kb on + strand at 969.317 kb on - strand at 969.317 kb on - strand at 969.458 kb on + strand, within Psest_0893 at 969.538 kb on + strand, within Psest_0893 at 969.539 kb on - strand, within Psest_0893 at 969.582 kb on + strand, within Psest_0893 at 969.683 kb on - strand, within Psest_0893 at 969.719 kb on + strand, within Psest_0893 at 969.719 kb on + strand, within Psest_0893 at 969.735 kb on + strand, within Psest_0893 at 969.736 kb on - strand, within Psest_0893 at 969.753 kb on - strand, within Psest_0893 at 969.753 kb on - strand, within Psest_0893 at 969.796 kb on + strand, within Psest_0893 at 969.796 kb on + strand, within Psest_0893 at 969.796 kb on + strand, within Psest_0893 at 969.797 kb on - strand, within Psest_0893 at 969.797 kb on - strand, within Psest_0893 at 969.813 kb on + strand, within Psest_0893 at 969.817 kb on + strand, within Psest_0893 at 969.817 kb on + strand, within Psest_0893 at 969.817 kb on + strand, within Psest_0893 at 969.817 kb on + strand, within Psest_0893 at 969.817 kb on + strand, within Psest_0893 at 969.817 kb on + strand, within Psest_0893 at 969.817 kb on + strand, within Psest_0893 at 969.817 kb on + strand, within Psest_0893 at 969.818 kb on - strand, within Psest_0893 at 969.818 kb on - strand, within Psest_0893 at 969.873 kb on + strand, within Psest_0893 at 969.874 kb on - strand, within Psest_0893 at 969.874 kb on - strand, within Psest_0893 at 969.916 kb on + strand, within Psest_0893 at 969.916 kb on + strand, within Psest_0893 at 969.916 kb on + strand, within Psest_0893 at 970.068 kb on + strand, within Psest_0893 at 970.068 kb on + strand, within Psest_0893 at 970.068 kb on + strand, within Psest_0893 at 970.068 kb on + strand, within Psest_0893 at 970.068 kb on + strand, within Psest_0893 at 970.068 kb on + strand, within Psest_0893 at 970.069 kb on - strand, within Psest_0893 at 970.069 kb on - strand, within Psest_0893 at 970.069 kb on - strand, within Psest_0893 at 970.070 kb on + strand, within Psest_0893 at 970.070 kb on + strand, within Psest_0893 at 970.070 kb on + strand, within Psest_0893 at 970.070 kb on + strand, within Psest_0893 at 970.070 kb on + strand, within Psest_0893 at 970.070 kb on + strand, within Psest_0893 at 970.070 kb on + strand, within Psest_0893 at 970.070 kb on + strand, within Psest_0893 at 970.071 kb on - strand, within Psest_0893 at 970.071 kb on - strand, within Psest_0893 at 970.071 kb on - strand, within Psest_0893 at 970.116 kb on - strand at 970.116 kb on - strand at 970.126 kb on + strand at 970.127 kb on - strand at 970.127 kb on - strand at 970.127 kb on - strand at 970.152 kb on + strand at 970.152 kb on + strand at 970.152 kb on + strand at 970.153 kb on - strand at 970.153 kb on - strand at 970.175 kb on + strand at 970.175 kb on + strand at 970.175 kb on + strand at 970.175 kb on + strand at 970.175 kb on + strand at 970.176 kb on - strand at 970.176 kb on - strand at 970.176 kb on - strand at 970.176 kb on - strand at 970.176 kb on - strand at 970.176 kb on - strand at 970.176 kb on - strand at 970.176 kb on - strand at 970.176 kb on - strand at 970.176 kb on - strand at 970.176 kb on - strand at 970.176 kb on - strand at 970.185 kb on - strand at 970.222 kb on + strand at 970.222 kb on + strand at 970.222 kb on + strand at 970.222 kb on + strand at 970.223 kb on - strand at 970.223 kb on - strand at 970.243 kb on + strand at 970.243 kb on + strand at 970.243 kb on + strand at 970.244 kb on - strand at 970.244 kb on - strand at 970.244 kb on - strand at 970.244 kb on - strand at 970.244 kb on - strand at 970.244 kb on - strand at 970.244 kb on - strand at 970.244 kb on - strand at 970.270 kb on + strand at 970.324 kb on + strand at 970.324 kb on + strand at 970.325 kb on - strand at 970.325 kb on - strand at 970.325 kb on - strand at 970.326 kb on + strand at 970.327 kb on - strand at 970.327 kb on - strand at 970.327 kb on - strand at 970.327 kb on - strand at 970.339 kb on + strand at 970.354 kb on + strand at 970.355 kb on - strand at 970.391 kb on - strand at 970.391 kb on - strand at 970.391 kb on - strand at 970.391 kb on - strand at 970.431 kb on + strand at 970.432 kb on - strand at 970.434 kb on - strand at 970.499 kb on - strand at 970.546 kb on + strand at 970.546 kb on + strand at 970.601 kb on + strand at 970.676 kb on + strand at 970.676 kb on + strand at 970.676 kb on + strand at 970.676 kb on + strand at 970.676 kb on + strand at 970.676 kb on + strand at 970.677 kb on - strand at 970.677 kb on - strand at 970.677 kb on - strand at 970.677 kb on - strand at 970.695 kb on + strand at 970.695 kb on + strand at 970.696 kb on - strand at 970.696 kb on - strand at 970.854 kb on - strand at 970.854 kb on - strand at 970.878 kb on + strand, within Psest_0895 at 970.878 kb on + strand, within Psest_0895 at 970.878 kb on + strand, within Psest_0895 at 970.878 kb on + strand, within Psest_0895 at 970.878 kb on + strand, within Psest_0895 at 970.878 kb on + strand, within Psest_0895 at 970.878 kb on + strand, within Psest_0895 at 970.878 kb on + strand, within Psest_0895 at 970.878 kb on + strand, within Psest_0895 at 970.878 kb on + strand, within Psest_0895 at 970.878 kb on + strand, within Psest_0895 at 970.878 kb on + strand, within Psest_0895 at 970.878 kb on + strand, within Psest_0895 at 970.878 kb on + strand, within Psest_0895 at 970.878 kb on + strand, within Psest_0895 at 970.878 kb on + strand, within Psest_0895 at 970.878 kb on + strand, within Psest_0895 at 970.879 kb on - strand, within Psest_0895 at 970.879 kb on - strand, within Psest_0895 at 970.879 kb on - strand, within Psest_0895 at 970.879 kb on - strand, within Psest_0895 at 970.879 kb on - strand, within Psest_0895 at 970.879 kb on - strand, within Psest_0895 at 970.879 kb on - strand, within Psest_0895 at 970.879 kb on - strand, within Psest_0895 at 970.879 kb on - strand, within Psest_0895 at 970.879 kb on - strand, within Psest_0895 at 970.879 kb on - strand, within Psest_0895 at 970.948 kb on + strand, within Psest_0895 at 970.948 kb on + strand, within Psest_0895 at 970.949 kb on - strand, within Psest_0895 at 970.949 kb on - strand, within Psest_0895 at 971.112 kb on + strand, within Psest_0895 at 971.112 kb on + strand, within Psest_0895 at 971.112 kb on + strand, within Psest_0895 at 971.112 kb on + strand, within Psest_0895 at 971.112 kb on + strand, within Psest_0895 at 971.113 kb on - strand, within Psest_0895
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined media remove 968,335 + +0.6 968,377 - +0.9 968,413 + -1.3 968,490 + Psest_0892 0.15 -1.0 968,490 + Psest_0892 0.15 -0.7 968,490 + Psest_0892 0.15 -1.3 968,490 + Psest_0892 0.15 -0.5 968,490 + Psest_0892 0.15 -0.1 968,491 - Psest_0892 0.15 -0.0 968,491 - Psest_0892 0.15 -1.1 968,491 - Psest_0892 0.15 +1.5 968,491 - Psest_0892 0.15 +1.0 968,491 - Psest_0892 0.15 +0.2 968,491 - Psest_0892 0.15 +0.2 968,620 + Psest_0892 0.31 -1.1 968,621 - Psest_0892 0.32 +0.7 968,649 + Psest_0892 0.35 -0.3 968,758 - Psest_0892 0.49 -0.0 968,780 - Psest_0892 0.52 -0.8 968,858 + Psest_0892 0.62 -1.3 968,859 - Psest_0892 0.62 -0.6 968,859 - Psest_0892 0.62 -0.6 968,859 - Psest_0892 0.62 -0.2 968,859 - Psest_0892 0.62 +0.6 968,859 - Psest_0892 0.62 -1.3 968,859 - Psest_0892 0.62 +0.2 968,859 - Psest_0892 0.62 -0.6 968,859 - Psest_0892 0.62 -0.9 968,859 - Psest_0892 0.62 -1.3 968,859 - Psest_0892 0.62 -0.0 968,874 + Psest_0892 0.64 +1.5 968,874 + Psest_0892 0.64 -0.2 968,875 - Psest_0892 0.64 +0.9 968,923 + Psest_0892 0.70 -2.1 968,923 + Psest_0892 0.70 -0.9 968,923 + Psest_0892 0.70 +0.5 968,924 - Psest_0892 0.70 -1.5 968,924 - Psest_0892 0.70 -0.9 968,924 - Psest_0892 0.70 -0.5 968,992 + Psest_0892 0.79 -0.0 968,993 - Psest_0892 0.79 -0.4 969,018 - Psest_0892 0.82 -0.7 969,145 - +1.6 969,184 + -0.5 969,185 - +0.5 969,185 - +0.3 969,185 - -0.4 969,206 - +0.1 969,230 - -2.0 969,269 - -1.8 969,287 - -0.3 969,316 + -2.6 969,316 + -1.5 969,316 + -1.4 969,317 - -0.9 969,317 - -3.4 969,458 + Psest_0893 0.21 -0.9 969,538 + Psest_0893 0.30 -2.8 969,539 - Psest_0893 0.30 -0.7 969,582 + Psest_0893 0.35 -2.2 969,683 - Psest_0893 0.46 -0.4 969,719 + Psest_0893 0.50 -2.3 969,719 + Psest_0893 0.50 +0.4 969,735 + Psest_0893 0.52 +0.3 969,736 - Psest_0893 0.52 -1.0 969,753 - Psest_0893 0.54 +0.1 969,753 - Psest_0893 0.54 -0.5 969,796 + Psest_0893 0.58 -0.5 969,796 + Psest_0893 0.58 -1.0 969,796 + Psest_0893 0.58 -0.4 969,797 - Psest_0893 0.58 -2.7 969,797 - Psest_0893 0.58 -1.0 969,813 + Psest_0893 0.60 +0.5 969,817 + Psest_0893 0.61 -0.3 969,817 + Psest_0893 0.61 -0.6 969,817 + Psest_0893 0.61 +0.3 969,817 + Psest_0893 0.61 -0.1 969,817 + Psest_0893 0.61 -0.1 969,817 + Psest_0893 0.61 -1.1 969,817 + Psest_0893 0.61 +0.6 969,817 + Psest_0893 0.61 -0.4 969,818 - Psest_0893 0.61 -1.9 969,818 - Psest_0893 0.61 -1.0 969,873 + Psest_0893 0.67 +0.8 969,874 - Psest_0893 0.67 -0.9 969,874 - Psest_0893 0.67 +0.7 969,916 + Psest_0893 0.71 -0.7 969,916 + Psest_0893 0.71 -0.8 969,916 + Psest_0893 0.71 -0.6 970,068 + Psest_0893 0.88 +0.4 970,068 + Psest_0893 0.88 -1.1 970,068 + Psest_0893 0.88 +0.1 970,068 + Psest_0893 0.88 -0.1 970,068 + Psest_0893 0.88 -0.6 970,068 + Psest_0893 0.88 -0.2 970,069 - Psest_0893 0.88 -0.6 970,069 - Psest_0893 0.88 -0.7 970,069 - Psest_0893 0.88 -1.0 970,070 + Psest_0893 0.88 +0.4 970,070 + Psest_0893 0.88 -0.4 970,070 + Psest_0893 0.88 -0.3 970,070 + Psest_0893 0.88 -0.8 970,070 + Psest_0893 0.88 -1.0 970,070 + Psest_0893 0.88 -0.3 970,070 + Psest_0893 0.88 +0.0 970,070 + Psest_0893 0.88 -1.0 970,071 - Psest_0893 0.88 -0.5 970,071 - Psest_0893 0.88 +0.0 970,071 - Psest_0893 0.88 +0.1 970,116 - -0.6 970,116 - -0.5 970,126 + +0.3 970,127 - -0.6 970,127 - +0.7 970,127 - -0.2 970,152 + -1.7 970,152 + -0.8 970,152 + -1.4 970,153 - -1.3 970,153 - -1.2 970,175 + -2.0 970,175 + -3.3 970,175 + -0.3 970,175 + -1.7 970,175 + -2.1 970,176 - +0.0 970,176 - +0.5 970,176 - -0.7 970,176 - +0.4 970,176 - -0.6 970,176 - -1.0 970,176 - -0.1 970,176 - -0.7 970,176 - +0.0 970,176 - +0.0 970,176 - -0.3 970,176 - -0.7 970,185 - +0.2 970,222 + -0.7 970,222 + +0.9 970,222 + -0.1 970,222 + -1.2 970,223 - +0.2 970,223 - -0.1 970,243 + -0.9 970,243 + +0.9 970,243 + -1.7 970,244 - -0.2 970,244 - -0.9 970,244 - -0.6 970,244 - -2.3 970,244 - -0.7 970,244 - +1.1 970,244 - +0.6 970,244 - +0.3 970,270 + -0.0 970,324 + -1.5 970,324 + -0.8 970,325 - -1.4 970,325 - -0.6 970,325 - -0.3 970,326 + -0.6 970,327 - +0.6 970,327 - -1.1 970,327 - -0.6 970,327 - -0.2 970,339 + -0.8 970,354 + -1.6 970,355 - -1.3 970,391 - -0.2 970,391 - -0.5 970,391 - +0.2 970,391 - -1.6 970,431 + -1.0 970,432 - -0.5 970,434 - -1.0 970,499 - -3.3 970,546 + -0.1 970,546 + -1.4 970,601 + -0.6 970,676 + -0.7 970,676 + +0.1 970,676 + -1.9 970,676 + -1.0 970,676 + -0.3 970,676 + +0.3 970,677 - -0.5 970,677 - -1.4 970,677 - -1.4 970,677 - +0.6 970,695 + -0.1 970,695 + +1.1 970,696 - +0.1 970,696 - -0.0 970,854 - -0.9 970,854 - -1.3 970,878 + Psest_0895 0.12 +0.2 970,878 + Psest_0895 0.12 +0.3 970,878 + Psest_0895 0.12 -0.1 970,878 + Psest_0895 0.12 -0.6 970,878 + Psest_0895 0.12 -0.0 970,878 + Psest_0895 0.12 +0.7 970,878 + Psest_0895 0.12 +0.2 970,878 + Psest_0895 0.12 +0.4 970,878 + Psest_0895 0.12 +0.4 970,878 + Psest_0895 0.12 +0.4 970,878 + Psest_0895 0.12 +0.5 970,878 + Psest_0895 0.12 -0.3 970,878 + Psest_0895 0.12 +0.6 970,878 + Psest_0895 0.12 -1.0 970,878 + Psest_0895 0.12 -0.0 970,878 + Psest_0895 0.12 +0.0 970,878 + Psest_0895 0.12 -0.3 970,879 - Psest_0895 0.12 -2.0 970,879 - Psest_0895 0.12 -1.5 970,879 - Psest_0895 0.12 -2.0 970,879 - Psest_0895 0.12 -0.5 970,879 - Psest_0895 0.12 -2.2 970,879 - Psest_0895 0.12 -2.0 970,879 - Psest_0895 0.12 +0.1 970,879 - Psest_0895 0.12 -2.3 970,879 - Psest_0895 0.12 +1.0 970,879 - Psest_0895 0.12 -1.0 970,879 - Psest_0895 0.12 -0.1 970,948 + Psest_0895 0.18 -0.4 970,948 + Psest_0895 0.18 -0.5 970,949 - Psest_0895 0.18 -1.8 970,949 - Psest_0895 0.18 -0.7 971,112 + Psest_0895 0.32 -0.2 971,112 + Psest_0895 0.32 -0.2 971,112 + Psest_0895 0.32 -0.7 971,112 + Psest_0895 0.32 -2.7 971,112 + Psest_0895 0.32 -0.2 971,113 - Psest_0895 0.32 -0.9
Or see this region's nucleotide sequence